We narrowed to 66,145 results for: %s
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Plasmid#199390PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag, biotin acceptor peptide (Avi), and SUMOEu tagDepositorInsertanti-ALFA nanobody
Tags14xHis-Avi-SUMOEuExpressionBacterialPromoterT5-LacOAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide Blast
Plasmid#199622PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and blasticidin resistance from EF-1a promoter.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-SauriABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189925PurposeAAV genome encoding SauriABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSauriABE8e
UseAAVMutationSauriCas9 D15APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-F-RAM-d2tTA-TRE-mKate2
Plasmid#140274PurposeF-RAM, the Fos-dependent reporterDepositorInsertmKate2
UseAAVPromoterTREAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEA1-Puro
Plasmid#171991PurposeDelivers all prime editing nickase components in a single, puromycin selectable plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
ExpressionMammalianPromoterCMV for Cas9, U6 for gRNAsAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-PalmGRET
Plasmid#158221PurposeBRET-based reporter to enable pan-extracellular particle labelling ranging from exomeres (< 50 nm) to small (< 200 nm; e.g. exosomes) and medium and large (> 200 nm; e.g. microvesicles) EVs.DepositorInsertPalmGFP-Nluc
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorMutationT2318C (DNA) in PEBV coat protein sequenceAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
RRL.sin.cPPT.SFFV/TMPRSS2 (variant 1).IRES-neo.WPRE (MT130)
Plasmid#145843PurposeLentiviral vector genome to ectopically express human TMPRSS2 variant 1DepositorInserthuman TMPRSS2 variant 1 (TMPRSS2 Human)
UseLentiviralExpressionMammalianMutationG8VPromoterSFFVAvailable sinceMay 14, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDONR223 SARS-CoV-2 NSP15
Plasmid#141268PurposeGateway-compatible Entry vector, with insert of NSP15 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP15 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR223 SARS-CoV-2 NSP8
Plasmid#141262PurposeGateway-compatible Entry vector, with insert of NSP8 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP8 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDONR223 SARS-CoV-2 NSP7
Plasmid#141261PurposeGateway-compatible Entry vector, with insert of NSP7 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorInsertNSP7 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
UseGateway CloningMutationMany synonymous changes due to codon optimizationAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsExpressionMammalianPromoterPcmvAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP
Plasmid#118156PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-EGFP
UseCRISPR and LentiviralTagsHAExpressionMammalianPromoterEF1a core and U6Available sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKM468-EGFP
Plasmid#108434PurposeORBIT integrating plasmid for C-terminal tagging with EGFP.DepositorInsertattB-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMetYC-Dest
Plasmid#105081PurposeYeast mating-based Split Ubiquitin Assay, bait vector for Gateway cloning. Cub-PLV does not contain a start codon.DepositorTypeEmpty backboneTagsCub-PLVExpressionYeastAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpCas9(BB)-2A-miRFP670
Plasmid#91854PurposeCas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988DepositorInsertshSpCas9-2A-miRFP670
BB-guide RNA
UseCRISPRTags2A-miRFP670 and 3XFLAGExpressionMammalianPromoterCbh and U6Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-hPR-A
Plasmid#89119Purposemammalian expression of full-length human progesterone receptor-A, equivalent to human PR-B amino acids 165-933DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman progesterone receptor-A (PGR Human)
ExpressionMammalianMutationhuman progesterone receptor-A, equivalent to huma…PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBK-YEE-BE3
Plasmid#85177PurposeExpresses YEE-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYEE-BE3
ExpressionMammalianPromoterCMVAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p5E-elavl3
Plasmid#75025PurposeCan be used to drive pan neuronal expression in zebrafish.DepositorPublicationInsertELAV like neuron-specific RNA binding protein 3 (elavl3 Zebrafish)
Use5' entry vector for multisite gateway three …Promoterelavl3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by