We narrowed to 30,447 results for: Ide

1. pU6-Decoy

   Plasmid

   #80324

   Purpose

   Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

   Insert

   Decoy sgRNA
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPO717

   Plasmid

   #195302

   Purpose

   pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

   Depositor

   Joseph Peters

   Article

   Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

   Insert

   McCAST Cascade and lacZ spacer 5 in native array
   

   Expression

   Bacterial

   Promoter

   Arabinose inducible arapBAD

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

   Plasmid

   #139649

   Purpose

   Reporter assay

   Depositor

   Yutaka Hata

   Article

   Kawano et al J Biochem. 2015 May 14. pii: mvv051.

   Insert

   H2B-mCherry
   

   Use

   Lentiviral

   Tags

   FLAG, His6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-InPAkt

   Plasmid

   #181914

   Purpose

   Indicator of Phosphoinositides using Akt; genetically encoded FRET-based sensor for monitoring 3-phosphoinositide dynamics in living cells.

   Depositor

   Jin Zhang

   Article

   Ananthanarayanan et al Proc Natl Acad Sci U S A. 2005 Oct 18;10

   Insert

   InPAkt
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSSX-STC

   Plasmid

   #115668

   Purpose

   E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

   Depositor

   David Kramer

   Article

   Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

   Insert

   small tetraheme cytochrome c
   

   Tags

   Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH-nuc-roGFP2-GPXC71S

   Plasmid

   #191760

   Purpose

   Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   nuc-roGFP2-GPXC71S
   

   Use

   Binary agrobacterium ti plasmid

   Expression

   Plant

   Mutation

   GPX has no peroxidatic Cysteine 71, which is repl…

   Promoter

   CaMV 35S

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGS_N_3xFLAG

   Plasmid

   #101654

   Purpose

   Expression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells

   Depositor

   Bjoern Sander, Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   LRP6 Signal peptide, KpnI linker, 3xFLAG tag
   

   Tags

   3xFLAG tag

   Expression

   Insect

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-X1-Neo-NDUFB2

   Plasmid

   #139841

   Purpose

   Lentiviral expression of human NDUFB2

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   NDUFB2 (NDUFB2 Human)
   

   Use

   Lentiviral

   Available Since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PKC-delta-IRES-hrGFP

   Plasmid

   #73414

   Purpose

   Mammalian expression of mouse PKC-delta and hrGFP

   Depositor

   Raymond Swanson

   Article

   Brennan-Minnella et al Cell Death Dis. 2013 Apr 4;4:e580. doi:

   Insert

   PKC-delta (Prkcd Mouse)
   

   Tags

   3xFLAG and IRES-hrGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

10. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

    Plasmid

    #129704

    Purpose

    Express HA-KASH for nuclei labeling and purification

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    HA-VHH-KASH
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. CLM RP HTR

    Plasmid

    #24430

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Available Since

    June 30, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pOPO635

    Plasmid

    #195297

    Purpose

    pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABC operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRV DYRK2

    Plasmid

    #12568

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pICH-chl-roGFP2-GPXC71S

    Plasmid

    #191703

    Purpose

    Ti plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-redox(ro)GFP2-GPXC71S
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Mutation

    Peroxidatic Cysteine 71 replaced with Serine

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. (K) tNOS (S)_T3 (SBE120)

    Plasmid

    #195010

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T3 position (K/S)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (K) tNOS (S)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. (H) tNOS (I) _T2 (SBE119)

    Plasmid

    #195006

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T2 position (H/I)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (H) tNOS (I)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. (E) tNOS (F) _T1 (SBE118)

    Plasmid

    #194997

    Purpose

    terminator from Agrobacterium tumefaciens nopaline synthase (tNOS, MF116010) for T1 position (E/F)

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    (E) tNOS (F)
    

    Expression

    Bacterial

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-flex-rev-g2-2A-Venus

    Plasmid

    #71410

    Purpose

    This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. Confers zolpidem sensitivity on zolpidem-insensitive neurons.

    Depositor

    William Wisden

    Article

    Uygun et al J Neurosci. 2016 Nov 2;36(44):11171-1118

    Inserts

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    mouse hdc gene promoter, works as a pan neuronal …

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPO636

    Plasmid

    #195299

    Purpose

    pACYC-plac-MycaTnsABCQ, lac promoter expressing transposase genes TnsABCQ of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABCQ operon of McCAST (Tn7575)
    

    Expression

    Bacterial

    Promoter

    IPTG inducible lac promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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