We narrowed to 31,013 results for: uros

1. pBabe puro pI3K p110 CAAX

   Plasmid

   #13339

   Depositor

   Channing Der

   Article

   McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

   Insert

   pI3K p110 CAAX (PIK3CA Bovine)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pBabe-TetCMV-puro-mVenus-LOV

   Plasmid

   #22033

   Purpose

   Mammalian expression of mVenus tagged LOV2 domain

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-LOV
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Insert length based on Venus (720bp) + LOV (450bp)

   Promoter

   Available since

   Sept. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

   Plasmid

   #115519

   Purpose

   To construct multiple sgRNA expression vector for CRISPR

   Depositor

   Xingxu Huang

   Insert

   ccdB-sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe puro-HA-PIK3CA(H1047R;I848V)

   Plasmid

   #33253

   Depositor

   Thomas Roberts

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   PIK3CA (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   changed histidine 1047 to arginine and isoleucine…

   Promoter

   SV40

   Available since

   Jan. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pRetroX-TetOne-Puro-p62 deltaUBA

   Plasmid

   #204550

   Purpose

   Expresses p62 deltaUBA in a doxycycline-dependent manner in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   p62 (SQSTM1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deltaUBA

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe puro H-Ras G12V Y40C

   Plasmid

   #12276

   Depositor

   Bob Weinberg

   Article

   Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

   Insert

   H-Ras G12V Y40C (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Contains G12V activating mutation. The Y40C mutat…

   Promoter

   Available since

   July 20, 2006

   Availability

   Academic Institutions and Nonprofits only

7. LIR_TRE_CAG_mKateII-Puro-STOP_mVenus-Cas9-PA-RIR

   Plasmid

   #68345

   Purpose

   A Sleeping beauty transposon with conditional expressed hCas9. A red flourescent gene linked to puromycin can be removed in the prescence of Flp recombinase allowing Cas9 expression

   Depositor

   Jannik Elverløv-Jakobsen

   Article

   Berthelsen et al Cancers (Basel). 2021 Jun 16;13(12). pii

   Inserts

   puromycin
   hCas9
   

   Use

   CRISPR; Flp/frt

   Tags

   linked to red flouresent protein gene mKateII and…

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLC-CUL4A K705R-P2A-Puro

   Plasmid

   #124305

   Purpose

   Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CUL4A (CUL4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Lysine 705 to Arginine

   Promoter

   CMV

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLG1-puro Non-targeting sgRNA 1

   Plasmid

   #109002

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-puro human lipin 1-1 shRNA

    Plasmid

    #32019

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-TRE3G-BE3-PGK-Puro

    Plasmid

    #110845

    Purpose

    Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    TRE3G

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe-puro/HA-FLAG-RBP2 H483A

    Plasmid

    #14803

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    changed His 483 to Ala

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. shPBRM1 #122-Tet-pLKO-puro

    Plasmid

    #107401

    Purpose

    Lentiviral expression of shRNA targeting PBRM

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    shRNA targeting PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-FLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172626

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. Direct-seq_lentiGuide-Puro-Loop2-8A8G

    Plasmid

    #157983

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Loop2-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetro-XT-PAK2-CFP(puro)-T402A

    Plasmid

    #81179

    Purpose

    Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Expression

    Mutation

    T402A

    Promoter

    P-tight TRE

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid

    #102904

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBabe-TetCMV-puro-mVenus-PA-Rac1

    Plasmid

    #22008

    Purpose

    Mammalian expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pLenti-CMV-PtenK13E/K289E-Puro

    Plasmid

    #135681

    Purpose

    Constitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection

    Depositor

    Florian Karreth

    Insert

    Pten (Pten Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K13E/K289E

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lv EF1a HP1cs-Frb2x PGK Puro

    Plasmid

    #102809

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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