We narrowed to 6,510 results for: itch

1. pBad-PhoCl2f

   Plasmid

   #164036

   Purpose

   Improved PhoCl variant with faster dissociation rate

   Depositor

   Robert Campbell

   Article

   Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

   Insert

   PhoCl2f
   

   Use

   Tags

   His tag, T7 tag, Xpress Tag

   Expression

   Bacterial

   Mutation

   Promoter

   araBad promotor

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbS5c-mCherry-AsLOV2*(543)

   Plasmid

   #202712

   Purpose

   Expresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)

   Depositor

   Mary Dunlop

   Article

   Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

   Insert

   mCherry-AsLOV2*(543)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eMagAF-EGFP-Mito

   Plasmid

   #162243

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagAF-EGFP-OMP25
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP64-membrane-mCherry-LOV

   Plasmid

   #135599

   Purpose

   Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   membrane-mCherry-LOV
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   LOV and mCherry

   Expression

   Mutation

   Promoter

   Unknown

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. RGG-BcLOV-mCh

   Plasmid

   #208276

   Purpose

   Expresses RGG-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   RGG-BcLOV-mCh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Fus-BcLOV-mCh

   Plasmid

   #208277

   Purpose

   Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

   Depositor

   Lukasz Bugaj

   Article

   Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

   Insert

   Fus-BcLOV-mCh
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Jan. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pXF276

   Plasmid

   #139048

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   lov domain (includes STOP codon)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBLind

   Plasmid

   #113109

   Purpose

   Vector encoding the RFP gene under blue light-inducible promoter BindF1.

   Depositor

   Chueh Loo Poh

   Article

   Jayaraman et al ACS Synth Biol. 2018 Apr 20;7(4):986-994

   Insert

   RFP
   

   Use

   Cell-free

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BindF1

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSDH52

   Plasmid

   #139049

   Purpose

   MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   epdz (includes STOP codon)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Endoplasmic reticulum-targeted optoPLD

    Plasmid

    #140060

    Purpose

    Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CIBN, CRY2, P2A, Sac1(TM), and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-K-CAAX

    Plasmid

    #41644

    Purpose

    Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)

    Depositor

    Michael Lin

    Article

    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

    Insert

    Dronpa145K-CAAX
    

    Use

    Tags

    CAAX box

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNO183

    Plasmid

    #101068

    Purpose

    ppsR2-bphP1 bicistronic operon plasmid

    Depositor

    Jeffrey Tabor

    Article

    Ong et al ACS Synth Biol. 2017 Nov 1. doi: 10.1021

    Inserts

    ppsR2 (ppsR2 Rhodopseudomonas palustris CGA009)
    bphP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    insert guanine at position 430 to fix frameshift …

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Endosome-targeted optoPLD

    Plasmid

    #140056

    Purpose

    Encodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    2xFYVE, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pADH110

    Plasmid

    #90982

    Purpose

    NAT-marked pSNR52 promoter fragment for use in gRNA expression cassette stitching PCR; for use with pADH119, 139, or 147 to generate target-specific gRNA expression cassette.

    Depositor

    Aaron Hernday

    Article

    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

    Inserts

    NAT 2 of 2
    pSNR52
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCBI-CRY2PHR-VPR

    Plasmid

    #192606

    Purpose

    Plasmid for constitutive expression of CRY2PHR-VPR.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    CRY2PHR-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. B2395_CIBN-BTB-IDR-FRB

    Plasmid

    #215107

    Purpose

    Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

    Depositor

    Chandra Tucker

    Article

    Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

    Insert

    CIBN-BTB-FUS(IDR)-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Golgi-targeted optoPLD

    Plasmid

    #140058

    Purpose

    Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NLS-GFP-iLID-Ferritin

    Plasmid

    #227376

    Purpose

    Condensate module, light-inducible oligomerization 'core'

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    GFP 'core'
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS_2paCRY2

    Plasmid

    #129651

    Purpose

    2P-activatable CRY2

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CLPIT Cry2PHR-mCh-LRP6c

    Plasmid

    #42961

    Depositor

    David Schaffer

    Article

    Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

    Insert

    Cry2PHR-mCherry-LRP6c
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    LPR6 cytoplasmic domain only

    Promoter

    Tet-Off promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSDH61

    Plasmid

    #139033

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    epdz (no STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL3-Basic-9xSeq2-Luc

    Plasmid

    #42214

    Purpose

    Luciferase reporter for GI-ZFP2

    Depositor

    Charles Gersbach

    Article

    Polstein et al J Am Chem Soc. 2012 Oct 10;134(40):16480

    Insert

    9xSeq2 Binding Sites
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    9 tandem copies of Seq2 + minimal human CMV promo…

    Available since

    Jan. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

    Plasmid

    #219654

    Purpose

    Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.

    Depositor

    Thomas Oertner

    Insert

    2xLyn-ERex-mKate2-bPAC(F198Y)
    

    Use

    AAV

    Tags

    ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pNP1 sfGFP trigger

    Plasmid

    #107359

    Purpose

    Trigger sequence for pNP1 sfGFP toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 7
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Golgi-targeted optodPLD

    Plasmid

    #140057

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA3-AKAP1-mCherry-LOVPLD

    Plasmid

    #219558

    Purpose

    mitochondria-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    AKAP1-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD/HisB-PhoCl-MBP

    Plasmid

    #87689

    Purpose

    Express PhoCl-MBP in E.coli

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl fused to Maltose-Binding Protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

    Plasmid

    #219555

    Purpose

    PM-targeted LOVPLD

    Depositor

    Jeremy Baskin

    Article

    Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

    Insert

    lyn10-mCherry-LOVPLD
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-PA(WT)-Vav2

    Plasmid

    #86977

    Purpose

    PI(DM): Photo-activatable/light sensitive

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTriEx-mCherry-LOV2 V416I

    Plasmid

    #81045

    Purpose

    mammalian expression of LOV V416I

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV V416I
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    V416I

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pTriEx-mCherry-LOV2 I427V

    Plasmid

    #81044

    Purpose

    mammalian expression of LOV2 I427V

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427V
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427V

    Promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. H2B-GFP-AsLOV2-NES27 (pDN158)

    Plasmid

    #72659

    Purpose

    Constitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear export

    Depositor

    Roland Eils, Barbara Di Ventura

    Article

    Niopek et al Nat Commun. 2016 Feb 8;7:10624. doi: 10.

    Insert

    H2B-GFP-AsLOV2-NES27
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Endosome-targeted optodPLD

    Plasmid

    #140055

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    2xFYVE, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQP-2473

    Plasmid

    #138976

    Purpose

    Nucleus-to-cytoplasm relocalization of genomically expressed GFP-fusion using NIR light controlled intrabody.

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-BphP1-NES-T2A- iB(GFP)-NES-mCherry-QPAS1- NLS-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT2/SVNeo-103

    Plasmid

    #79841

    Purpose

    Expresses RpBphP1-mCherry-TetR under CMVd1 promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    RpBphP1-mCherry-Intersectin1 DHPH domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVd1

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNP1 eforRed trigger

    Plasmid

    #107357

    Purpose

    Trigger sequence for pNP1 eforRed toehold switch

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold trigger 1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNUT3

    Plasmid

    #175744

    Purpose

    Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

    Depositor

    Yubin Zhou

    Article

    Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

    Insert

    mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 2xsspB-mSc-sspB-ITSN1

    Plasmid

    #114425

    Purpose

    Translocating ITSN1 to iLID and activate Cdc42

    Depositor

    Dorus Gadella

    Article

    Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

    Insert

    ITSN1
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    truncated ITSN1 (1159-1509)

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMTG07-Amp-FRT

    Plasmid

    #128276

    Purpose

    Gene circuit with the following architecture: 5xUAS-mNeonGreen--CMV-Gal4-VVD-p65

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

    Insert

    Gal4-VVD-p65
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge