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We narrowed to 9,678 results for: Gnas

Showing: 6701 - 6750 of 9678 results

  1. pcDNA3.1(+)-DnaJB1-PKAcat(K72H)-mCherry

    Plasmid
    #181851
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; contains catalytically dead PKA; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat(K72H)-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Lysine 128 in the DnaJB1-PKAcat fusion changed to…
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181851-plasmid-map-sequence-id-364311

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  4. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid
    #214278
    Purpose
    Express split-Cdc42 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Cdc42-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214278-plasmid-map-sequence-id-422249

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  5. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid
    #156415
    Purpose
    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12D (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mTagBFP2
    Expression
    Mutation
    mutant KRAS4A-G12D
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156415-plasmid-map-sequence-id-308711

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  7. pCEP4-myc-PDGFRA-R914W_EGFP-PEST_reporter

    Plasmid
    #172596
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-R914W
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172596-plasmid-map-sequence-id-338758

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  9. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid
    #156416
    Purpose
    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4A-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mScarlet-I
    Expression
    Mutation
    mutant KRAS4A-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156416-plasmid-map-sequence-id-308708

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  15. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid
    #156414
    Purpose
    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Joshua Wythe
    Article
    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC
    Insert
    human mutant KRAS4B-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    mutant KRAS4B-G12V
    Promoter
    kdrl
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    156414-plasmid-map-sequence-id-308707

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  19. pCDH-3_Flag-YTHDF2-RNA-MUT (nonsense mutation of sgYTHDF2-1)

    Plasmid
    #232948
    Purpose
    Expresses the RNA-binding-deficient mutant of YTHDF2 with synonymous mutations for resistance against sgYTHDF2-1-mediated knockdown
    Depositor
    Jianjun Chen
    Article
    Chen et al Cell. 2024 Dec 12:S0092-8674(24)01324-2.
    Insert
    YTHDF2-RNA-MUT (YTHDF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    RNA binding mutation (K416A+R527A), synonymous mu…
    Promoter
    Available since
    May 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232948-plasmid-map-sequence-id-473044

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  20. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid
    #214279
    Purpose
    Express split-Cdc42 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Cdc42-T17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214279-plasmid-map-sequence-id-422248

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  21. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid
    #214280
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (on insert 1) and mVenus (on insert 2)
    Expression
    Mammalian
    Mutation
    Rac1-Q61L
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214280-plasmid-map-sequence-id-422247

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  22. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid
    #214281
    Purpose
    Express split-Rac1 fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    Rac1-T17N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214281-plasmid-map-sequence-id-422246

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  23. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(T19N)-FKBP-mVenus-CAAX

    Plasmid
    #214283
    Purpose
    Express split-RhoA fragments that are fused with CIDs and FPs
    Depositor
    Cliff Stains
    Article
    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202
    Inserts
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    Use
    Tags
    mCerulean (N terminal on insert 1) and mVenus (on…
    Expression
    Mammalian
    Mutation
    RhoA-T19N
    Promoter
    CMV
    Available since
    July 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214283-plasmid-map-sequence-id-422243

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  25. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  26. pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)

    Plasmid
    #192704
    Purpose
    Expression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Use
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPm…
    Expression
    Mammalian
    Mutation
    meGFPm: gluatmic acid 142 to asparagine and histi…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192704-plasmid-map-sequence-id-379659

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  27. pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)

    Plasmid
    #192784
    Purpose
    Expression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Use
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and mXFPm
    Expression
    Mammalian
    Mutation
    mXFPm: tryptophan 66 to phenylalanine, gluatmic a…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    192784-plasmid-map-sequence-id-379372

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  28. pCEP4-myc-PDGFRA-D842V_EGFP-PEST_reporter

    Plasmid
    #172598
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-D842V (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172598-plasmid-map-sequence-id-338911

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  29. pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter

    Plasmid
    #172599
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-D842V+R914W
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172599-plasmid-map-sequence-id-338914

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  30. pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter

    Plasmid
    #172600
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R+R914W (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R+R914W
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172600-plasmid-map-sequence-id-338836

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  31. pCEP4-myc-PDGFRA-K627R_EGFP-PEST_reporter

    Plasmid
    #172597
    Purpose
    Mammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R mutant signaling.
    Depositor
    Daniel Kiss
    Article
    Yu et al Hum Mol Genet. 2022 Jul 21;31(14):2348-2
    Insert
    myc-PDGFRA-K627R (PDGFRA Human, Synthetic)
    Use
    Egfp-pest_reporter
    Tags
    Extracellular c-myc epitope tag and Signal peptid…
    Expression
    Mammalian
    Mutation
    PDGFRA-K627R
    Promoter
    CMV
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172597-plasmid-map-sequence-id-338908

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  32. pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181855
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181855-plasmid-map-sequence-id-362798

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  33. pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry

    Plasmid
    #181854
    Purpose
    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.
    Depositor
    Jin Zhang
    Article
    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.
    Insert
    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
    Promoter
    CMV
    Available since
    July 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181854-plasmid-map-sequence-id-362794

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  34. GFP-Cstem(7A)-Tac(5A)

    Plasmid
    #162499
    Purpose
    Expresses GFP tagged Tac mutant in mammalian cells. The five O-glycosylation sites in the stem region are mutated, and the stem region of CD8a with mutations is inserted between GFP and Tac(5A).
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    CD8a stem region with mutations and Tac full length with mutated stem region (IL2RA, CD8A Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    The five theronine and two serine residues are mu…
    Promoter
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    162499-plasmid-map-sequence-id-323491

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  48. TRUPATH Triple GaoA

    Plasmid
    #196051
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoA-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196051-plasmid-map-sequence-id-385865

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  49. TRUPATH Triple GaoB

    Plasmid
    #196052
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196052-plasmid-map-sequence-id-387470

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Showing: 6701 - 6750 of 9678 results