We narrowed to 4,337 results for: U6 gRNA

1. Adeno EA

   Plasmid

   #64071

   Purpose

   Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

   Depositor

   Andrea Ventura

   Article

   Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

   Insert

   U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
   

   Use

   Adenoviral

   Tags

   FLAG-Cas9

   Expression

   Mutation

   Promoter

   U6 and CBh

   Available since

   July 23, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pX459-CTNNB1-ATG

   Plasmid

   #153429

   Purpose

   Expresses a gRNA that overlaps the startcodon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCAS9 wt (BB)-2A-GFP targeting human TRIM21

   Plasmid

   #138295

   Purpose

   gRNA for CRISPR/Cas9 knockout of human TRIM21

   Depositor

   Gaudenz Danuser

   Article

   Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

   Insert

   gRNA TRIM21 (TRIM21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR Human CASP9 -2

   Plasmid

   #198420

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR Human CASP9 -1

   Plasmid

   #198419

   Purpose

   Expresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter Protein

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   Human CASP9 Specific gRNA (CASP9 Human)
   

   Use

   CRISPR

   Tags

   Cas9/Orange Fluorescent Protein Reporter

   Expression

   Mammalian

   Mutation

   Promoter

   U6; CMV

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX459-CTNNB1-S45

   Plasmid

   #164587

   Purpose

   Expresses a gRNA that overlaps the S45 codon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSI-359

   Plasmid

   #131131

   Purpose

   gRNA expression vector for A-to-G base editing reporter

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   A-to-G reporter activation gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. H513 px330.sgActin.UTR.2

    Plasmid

    #138176

    Purpose

    CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

    Depositor

    Wen Xue

    Article

    Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

    Insert

    sgActin (Actb Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

    Plasmid

    #188541

    Purpose

    Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

    Depositor

    Darrell Kotton

    Article

    Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

    Insert

    gRNA
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSI-535

    Plasmid

    #131130

    Purpose

    gRNA expression vector for C-to-T base editing reporter

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    C-to-T reporter activation gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDS48

    Plasmid

    #241839

    Purpose

    Cas9-gRNA construct targeting the UBE3A locus

    Depositor

    Albert Keung

    Article

    Kelkar et al Front Cell Dev Biol. 2025; 13:1665693. h

    Insert

    Cas9-gRNA (UBE3A )
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pX458-ARHGEF7/B-Pix

    Plasmid

    #241368

    Purpose

    Mammalian expression of SpCas9 and gRNA targeting ARHGEF7 (β-Pix)

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    ARHGEF7 gRNA (ARHGEF7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid

    #182678

    Purpose

    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Rat, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken beta-actin promoter

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJW1310

    Plasmid

    #61253

    Purpose

    Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    U6 promoter
    

    Use

    CRISPR; Cloning template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV.5’eGFP/sgVIM

    Plasmid

    #170548

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of VIM
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMSCV-miR30-Vector

    Plasmid

    #171194

    Purpose

    Retroviral vector for U6 promoter driven expression empty miR30 based shRNA (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    shRNA empty vector
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCKO2

    Plasmid

    #125518

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSB700

    Plasmid

    #64046

    Purpose

    Lentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent protein

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CAGGS

    Available since

    May 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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