We narrowed to 42,599 results for: cha;

1. pCAG-cofilin-S3D

   Plasmid

   #78297

   Purpose

   Mammalian expression of mouse cofilin S3D fused to EGFP

   Depositor

   Michael Frotscher

   Article

   Chai et al Development. 2016 Mar 15;143(6):1029-40.

   Insert

   cofilin 1 (Cfl1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S3D

   Promoter

   CAG

   Available since

   May 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA-Sumo1 Q94P

   Plasmid

   #48965

   Purpose

   Expresses HA SUMO1 Q94P mutant in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutated Q94P

   Promoter

   T7

   Available since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pC-HYG-YR

   Plasmid

   #61765

   Purpose

   Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

   Depositor

   Ken Haynes

   Article

   Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

   Inserts

   Hygromycin
   2 micron origin or replication and URA3 gene for S. cerevisiae
   

   Use

   Ternary vector for agrobacterium mediated transfo…

   Tags

   Expression

   Mutation

   Promoter

   trpC promoter

   Available since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-Lyl1

   Plasmid

   #131612

   Purpose

   expression of mouse LyI1 in mammalian cells

   Depositor

   Filipe Pereira, Kateri Moore

   Article

   Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

   Insert

   Lyl1 (Lyl1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJS288

   Plasmid

   #200714

   Purpose

   To express yeast Hxt1 hexose transporter in yeast

   Depositor

   Jens Boch

   Article

   Fuhrmeister et al Bio Protoc. 2023 Aug 5;13(15):e4733. doi

   Inserts

   PMA1 promoter (PMA1 Budding Yeast)
   Hxt1 (HXT1 Budding Yeast)
   ADH2 Ter (ADH2 Budding Yeast)
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   domesticated for MoClo cloning (removal of intern…

   Promoter

   Available since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOPINK-OTUD1 (OTU+UIM, aa 287-481)

   Plasmid

   #61406

   Purpose

   Expresses human OTUD1 (OTU+UIM domains) in E. coli.

   Depositor

   David Komander

   Article

   Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

   Insert

   OTUD1 (OTUD1 Human)
   

   Use

   Tags

   His6-GST-3C

   Expression

   Bacterial

   Mutation

   Deleted aa 1-286.

   Promoter

   T7

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

   Plasmid

   #24549

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

   Plasmid

   #176745

   Purpose

   Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

   Depositor

   Baljit Khakh

   Article

   Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

   Insert

   Glt1-BioID2-BioID2-HA
   

   Use

   AAV

   Tags

   HA

   Expression

   Bacterial

   Mutation

   Promoter

   gfaABC1D

   Available since

   April 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM113

   Plasmid

   #133666

   Purpose

   Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

    Plasmid

    #24584

    Depositor

    Thomas Wandless

    Article

    Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

    Insert

    pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    1. Truncated version of ADH700 promoter (SbfI/Spe…

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCRISPRv1_UQCRC2

    Plasmid

    #177982

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting UQCRC2

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting UQCRC2 (UQCRC2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs_FLAG-GGC1

    Plasmid

    #133258

    Purpose

    Express FLAG-tagged GGC1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    GGC1 (GGC1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

    Plasmid

    #186835

    Purpose

    Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513/515/516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mEos2-Actin-7

    Plasmid

    #57339

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. p2xFlag CMV2-YAP2-1st&2nd WW mutant

    Plasmid

    #19048

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine,and Proline 202…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV CaMKIIa iChloC 2A tDimer

    Plasmid

    #66709

    Purpose

    Chloride-conducting Channelrhodopsin with neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Wietek et al Sci Rep. 2015 Oct 7;5:14807. doi: 10.103

    Inserts

    Channelrhodopsin-2
    red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E83Q,E90R,E101S,D156N,T159C

    Promoter

    CaMKIIa

    Available since

    Oct. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGS1021

    Plasmid

    #59575

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a TRP1 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    Twin-Strep tag optimized for PCR amplification followed by TRP1 selection marker
    

    Use

    E. coli cloning vector

    Tags

    Twin-Strep tag

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pZac2.1 gfaABC1D-Cx43-BioID2-BioID2-HA

    Plasmid

    #176743

    Purpose

    Expresses Connexin-43 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Cx43-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYZ145

    Plasmid

    #98405

    Purpose

    Donor DNA plasmid to introduce leu1 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete leu1 in genome by recombination (apc2 Fission Yeast)
    downstream homologous region to delete leu1 in genome by recombination (top2 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYZ149

    Plasmid

    #98407

    Purpose

    Donor DNA plasmid to introduce his3 deletion in S. pombe. Linearization with Not1 digestion.

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Inserts

    upstream homologous region to delete his3 in genome by recombination (alg2 Fission Yeast)
    downstream homologous region to delete his3 in genome by recombination (cog8 Fission Yeast)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmCherry-C1-FAK-HA-V744G

    Plasmid

    #35040

    Depositor

    Anna Huttenlocher

    Article

    Chan et al J Biol Chem. 2010 Apr 9;285(15):11418-26

    Insert

    focal adhesion kinase (Ptk2 Mouse)
    

    Use

    Tags

    2x HA and mCherry

    Expression

    Mammalian

    Mutation

    GFP-FAK-V744G was generated from GFP-FAK using th…

    Promoter

    Available since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMG-miR-125b-1

    Plasmid

    #58992

    Purpose

    MSCV based expression vector - co-expresses GFP and human miR-125b-1

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    miR-125b-1 (MIR125B1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. HC078 (3xHA-Atg13)

    Plasmid

    #59544

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

    Depositor

    Daniel Klionsky

    Article

    Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    3xHA

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pIRES_EGFP_GA_KCNE1

    Plasmid

    #173160

    Purpose

    For mammalian expression of KCNE1 and creating variants

    Depositor

    Al George

    Article

    Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

    Insert

    KCNE1 (KCNE1 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-LGC-53

    Plasmid

    #86399

    Purpose

    Mammalian expression of codon-optimized dopamine receptor LGC-53

    Depositor

    Steven Mennerick

    Article

    Kress et al J Neurosci. 2014 Aug 27;34(35):11792-802

    Insert

    LGC-53 (lgc-53 Nematode, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression; Signal …

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMXs_FLAG-NDT1

    Plasmid

    #133254

    Purpose

    Express FLAG-tagged NDT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT1 (YIA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEBG-PELP1

    Plasmid

    #65209

    Purpose

    Full length mammalian expression vector for hPELP1

    Depositor

    Ratna Vadlamudi

    Article

    Nair et al Cancer Res. 2010 Sep 15;70(18):7166-75.

    Insert

    PELP1 (PELP1 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pCfB3052(gRNA X-4, XI-3, XII-5)

    Plasmid

    #73294

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOPINK-OTUD5 (OTU, aa 171-358)

    Plasmid

    #61415

    Purpose

    Expresses human OTUD5 (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-170 and aa 359-571.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGAN201

    Plasmid

    #129204

    Purpose

    yTRAP sensor of [RNQ+], detects aggregation of Rnq1

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    RNQ1 (RNQ1 Synthetic, Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOPINK-OTUD3 (OTU+UBA, aa 52-275)

    Plasmid

    #61411

    Purpose

    Expresses human OTUD3 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD3 (OTUD3 Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-51 and aa 276-398.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F RSR_AAA (Opt β-Gluclv2.0)

    Plasmid

    #47542

    Purpose

    engineered receptor improves heteromeric receptor expression and sensitivity to IVM.

    Depositor

    Henry Lester

    Article

    Frazier et al J Biol Chem. 2013 May 29.

    Insert

    Glutamate-gated chloride channels
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    retention motif in ER (starting at R340) changed …

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-DONSON

    Plasmid

    #208797

    Purpose

    Xenopus laevis His6-Myc-DONSON wild type

    Depositor

    Aga Gambus

    Article

    Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

    Insert

    DONSON (donson.L Frog)
    

    Use

    Tags

    His and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFL-EGFP-3xFlag-Fml1

    Plasmid

    #193058

    Purpose

    Baculovirus expression of S.pombe Fml1 for recombinant protein production

    Depositor

    Andrew Deans

    Article

    Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

    Insert

    Fml1 (fml1 Fission Yeast)
    

    Use

    Tags

    3xFlag

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. yHS1-M7A

    Plasmid

    #159151

    Purpose

    Cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    yHS1-M7A
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of yHS1 to A…

    Promoter

    GPD

    Available since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

    Plasmid

    #72888

    Purpose

    fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

    Insert

    Gaussia luciferase, Channelrhodopsin-2, EYFP
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. CS2 luc Fstl1

    Plasmid

    #13497

    Depositor

    Stephen Tapscott

    Article

    Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

    Insert

    Fstl1 (Fstl1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    contains 3 tandem copies of the following sequenc…

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

39. AAT-PB-CG2APtk

    Plasmid

    #86003

    Purpose

    homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

    Insert

    eGFP_T2A_puro_dTK
    

    Use

    Piggybac exciseable

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAf-CRISPR-phoA

    Plasmid

    #207991

    Purpose

    CRISPR vector used with pAf-CRISPR-ctfR1 (#207992) to delete both aflatoxin and cyclopiazonic acid gene clusters of Aspergillus flavus

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2023 Dec 27;170:10386

    Insert

    phoA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus flavus U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge