We narrowed to 9,586 results for: Coli
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Plasmid#193862PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged proteins. Empty backbone for LIC cloning.DepositorTypeEmpty backboneTagsGFP11, Hisx6, and R9ExpressionBacterialAvailable SinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only
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His SUMO TEV W131G SPOP 1-374
Plasmid#197019PurposeE. coli expression vectorDepositorAvailable SinceApril 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV E78K SPOP 1-374
Plasmid#197018PurposeE. coli expression vectorDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV E47K SPOP 1-374
Plasmid#197017PurposeE. coli expression vectorDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
His SUMO TEV W22R SPOP 1-374
Plasmid#197016PurposeE. coli expression vectorDepositorAvailable SinceApril 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-Bio2_MVPI_SMAC(delta(55))_9His
Plasmid#197978PurposeE. coli expression of MVPI-SMAC(delta(55))_9HisDepositorAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNIC-Bio2_SMAC(delta(55))_Cys_9His
Plasmid#197977PurposeE. coli expression of SMAC(delta(55))_Cys-9HisDepositorAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt1)-DadR(ΔDBD)
Plasmid#191637PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔDBD) complementationDepositorInsertDadR
ExpressionBacterialMutationΔDBDAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt2)-DadR(ΔTM)
Plasmid#191638PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(ΔTM) complementationDepositorInsertDadR
ExpressionBacterialMutationΔTMAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt4)-DadR(linker)
Plasmid#191639PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), dadR(linker region shuffled) complementationDepositorInsertDadR
ExpressionBacterialMutationLinker region sequence shuffledAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadR-NFLAG)-N-3xFLAG-DadR
Plasmid#191640PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR complementationDepositorInsertDadR
Tags3xFLAGExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
mRuby3 only VLP
Plasmid#182429PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-mRuby3
deltaVP1
UseSynthetic BiologyExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
QUAS-0-T7-TetO-GFP_ColE1
Plasmid#171662PurposeQUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.DepositorInsertQUAS-T7-TetO-GFP ssrA-T7 Stop
TagsssrA degradation tag (DAS+4)ExpressionBacterialPromoterT7Available SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET_His6-MBP-TEV-Cas6-RT-Cas1_WT
Plasmid#186274PurposeExpresses Thiomicrospira Cas6-RT-Cas1 in E. coliDepositorInsertThiomicropira Cas6-RT-Cas1
TagsHis6-MBPExpressionBacterialAvailable SinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaA
Plasmid#167810PurposeExpresses His6-tagged BhaA in E. coliDepositorInsertBhaA
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB1
Plasmid#167811PurposeExpresses His6-tagged BhaB1 in E. coliDepositorInsertBhaB1
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB7
Plasmid#167813PurposeExpresses His6-tagged BhaB7 in E. coliDepositorInsertBhaB7
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-bhaC1
Plasmid#167814PurposeExpresses BhaC1 in E. coliDepositorInsertBhaC1
ExpressionBacterialPromoterT7Available SinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
ExpressionBacterialMutationS83C, Δ157-178PromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
TagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialPromoterT7Available SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaK-IR2-IR2Available SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR3 GFP pZa
Plasmid#170084PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaK-IR3Available SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2 GFP pZa
Plasmid#170081PurposeGFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyExpressionBacterialPromoterPdnaK-IR2Available SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
TagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
ExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Plasmid#162965PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Halotag fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-tetpA(R1R4)-iRPT
Plasmid#154882PurposeUbiquitously expresses iRFP713, Puromycin resistance gene, and rtTAM2.DepositorInsertsiRFP713
Puromycin resistance gene
rtTAM2
ExpressionMammalianAvailable SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Avi-Coh (E154C)-HIS
Plasmid#153447PurposeE. coli expression of Rc. CohE construct with E154C mutation.DepositorInsertRc.CohE
Tags6xHis and Avi-tagExpressionBacterialMutationE154C mutation on CohEPromoterT7 promoterAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-tglA(C51)HIB
Plasmid#134675PurposeExpresses His6-TglA(C51), TglH, TglI and TglB in E. ColiDepositorInsertsTglA(C51)
TglH
TglI
TglB
TagsHis6ExpressionBacterialMutationinsertion of cysteine 51PromoterT7Available SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-ammA(∆50-56)
Plasmid#134684PurposeExpresses His6-AmmA(∆50-56) in E. ColiDepositorInsertammA(del50-56)
TagsHis6ExpressionBacterialMutationdeletion of amino acids 50-56PromoterT7Available SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis8-4b-McDAH
Plasmid#140129PurposeExpresses McDAH with N-terminal 8xHis-tag in BL21 E. coliDepositorInsertDechloroacutumine halogenase from Menispermum canadense
Tags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialPromoterT7Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSB4C5_J23101-vdh
Plasmid#128130PurposeExpressing vdh gene (for the enzyme transforming benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)
Plasmid#132637Purposeexpresses mouse Tankyrase1 ARC2-3 (308-655) in E. coliDepositorInsertTankyrase1 (308-655)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-vdh
Plasmid#128134PurposeThe cell-free adapted backbone, pBEAST, expressing vdh (the enzyme converting benzaldehyde to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertvdh
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_SsCHAD-H29A-R32A-R36A
Plasmid#127786PurposeExpresses a SsCHAD mutant protein in E. coli cellsDepositorInsertSsCHAD (SULM_RS11045 Sulfolobus solfataricus)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationmutations in His29, Arg32 and Arg36 to AlaAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH-HT_CtCHAD-R438A-K441A
Plasmid#127791PurposeExpresses a CtCHAD mutant protein in E. coli cellsDepositorInsertCtCHAD (CT0884 Chlorobium tepidum)
Tags6xHis tag + TEV cleavage siteExpressionBacterialMutationResidues 208-522 (CHAD domain), mutations in Arg4…Available SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI177
Plasmid#126077PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP1-10 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP1-10
UseBinary, module 1Tagslinker (GTGGGGS)ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI129
Plasmid#126210PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x7 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinary, module 1ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTEI161
Plasmid#126211PurposeModule 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11 and expression in plant cellDepositorInsertsccdB Cassette (BsaI)
GFP11
UseBinary, module 1ExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK2013-BsCas12b
Plasmid#121952PurposeE. coli expression, protein purificationDepositorInsertBsCas12b
Tags10xHis tag and 3xHA tagExpressionBacterialAvailable SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b+R2(L171C)
Plasmid#108239PurposeExpresses PdR-PCNA2(L171C) fusion protein in E. coliDepositorInsertPdR-PCNA2(L171C) fusion protein
TagsHis6 tagExpressionBacterialMutationPCNA2,L171CPromoterT7 promoterAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CTL2 (M2225R)
Plasmid#96952PurposeExpresses C-Terminal segment of C. elegans Piezo1 with M2225R mutation in E. coliDepositorInsertCTL2 (M222R) of PIEZO1
Tags6x Histidine TagExpressionBacterialPromoterT7 PromoterAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSM13
Plasmid#101644PurposeEncodes the fluorescent protein meleCFP for constitutive expression in E. coli.DepositorInsertmeleCFP
Tags6XHisExpressionBacterialAvailable SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21a - TryX
Plasmid#89623Purpose[Edit] Expresses recombinant 6HIS tagged T. cruzi tryparedoxin protein in E. coli upon IPTG inductionDepositorInsertTryparedoxin
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPD3cC
Plasmid#86972PurposeExpresses PCNA3 variant (R112C/T180C) fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 variant (R112C/T180C) fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R/R112C/T180C in PCNA3, …PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1G108CP20X
Plasmid#85096PurposeExpresses PCNA1 variant (G108C) fused to putidaredoxin in E. coliDepositorInsertThe G108C variant of PCNA1 fused to putidaredoxin
TagsHis6 tagExpressionBacterialMutationG108C in PCNA1PromoterT7 promoterAvailable SinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-pcDronpa
Plasmid#78183PurposeExpresses pcDronpa in E. coli strainsDepositorInsertpcDronpa
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK278
Plasmid#71134PurposeExpression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-89 to asparagine; additional N-…PromoterT7Available SinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only