4,177 results

1. pcDNA3 GFP LIC cloning vector (6D)

   Plasmid

   #30127

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV4-p52

   Plasmid

   #23289

   Depositor

   Warner Greene

   Article

   Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

   Insert

   p52 (NFKB2 Human)
   

   Expression

   Mammalian

   Available Since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. EMTB-mCherry

   Plasmid

   #26742

   Depositor

   William Bement

   Article

   Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

   Insert

   ensconsin (MAP7 Human)
   

   Use

   Probe for microtubules

   Tags

   2x mCherry and Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Available Since

   Feb. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV6M-Pak1 K299R

   Plasmid

   #12210

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Catalytically inactive Pak1: K299R

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV6M-PAK1 T423E

   Plasmid

   #12208

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Activated Pak1: T423E

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-Puro (PX462)

   Plasmid

   #48141

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mEmerald-N1

   Plasmid

   #53976

   Purpose

   Localization: N1 Cloning Vector, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   mEmerald

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Cdc2-HA

   Plasmid

   #1888

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdc2 (CDK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-p150Glued

   Plasmid

   #36154

   Depositor

   David Stephens

   Article

   Watson et al J Cell Sci. 2006 Jul 1;119(Pt 13):2758-6

   Insert

   p150Glued (DCTN1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   see documentation

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSXneo 270(T2AG3)

    Plasmid

    #12403

    Depositor

    Titia de Lange

    Article

    Hanish et al Proc Natl Acad Sci U S A. 1994 Sep 13. 9

    Insert

    270(T2AG3)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX601-GFP

    Plasmid

    #84040

    Purpose

    Staphylococcus aureus (SaCas9) conjugated with GFP

    Depositor

    Yuet Wai Kan

    Article

    Ye et al Proc Natl Acad Sci U S A. 2016 Sep 20;11

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    NLS and T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-OptoSTIM1

    Plasmid

    #70159

    Purpose

    Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

    Depositor

    Won Do Heo

    Article

    Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

    Insert

    OptoSTIM1 (STIM1 Mustard Weed, Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    T3

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

    Plasmid

    #37120

    Purpose

    DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    TdTomato-EGFP
    

    Use

    AAV and Cre/Lox; Cre-switch

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. NL4-3 mCherry Luciferase

    Plasmid

    #44965

    Purpose

    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

    Depositor

    Warner Greene

    Article

    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

    Insert

    mCherry, Luciferase, IRES
    

    Use

    Lentiviral

    Tags

    Luciferase, IRES and mCherry with D212G

    Mutation

    S34C mutation in Nef

    Available Since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Beta-arrestin2 GFP WT

    Plasmid

    #35411

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

    Insert

    βarrestin2 (Arrb2 Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMYC-GFP

    Plasmid

    #42142

    Depositor

    Olufunmilayo Olopade

    Article

    Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

    Insert

    c-Myc (MYC Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEBG

    Plasmid

    #22227

    Purpose

    GST fusion vector

    Depositor

    David Baltimore

    Article

    Tanaka et al Mol Cell Biol. 1995 Dec . 15(12):6829-37

    Type

    Empty backbone

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP-Rac1-T17N

    Plasmid

    #12982

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Rac1 DN (RAC1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T17N Dominant negative

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. p2CT-His-MBP-Lbu_C2c2_WT

    Plasmid

    #83482

    Purpose

    Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_WT
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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