We narrowed to 23,099 results for: crispr

1. p2CT-His-MBP-Lbu_C2c2_K1082A

   Plasmid

   #91863

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1082A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1082A

   Promoter

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas2D

   Plasmid

   #82375

   Purpose

   dCas9 expressed from aTc-inducible promoter in acsA, D RBS: AGGACG

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   dCas9
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   PEZ3

   Available Since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330.LoxP

   Plasmid

   #66586

   Purpose

   sgRNA for LSL

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   LoxP sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pFETCh_ZNF503

   Plasmid

   #135735

   Purpose

   Donor vector for 3' FLAG tag of human ZNF503

   Depositor

   Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   ZNF503 Homology arms (ZNF503 Human)
   

   Use

   CRISPR

   Tags

   3XFLAG-P2A-NeoR

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330.iGFP2

   Plasmid

   #66582

   Purpose

   sgRNA for iGFP

   Depositor

   Wen Xue

   Article

   Li et al Genome Biol. 2015 May 28;16(1):111.

   Insert

   iGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pKFSS1_Psyn-sgRNA500

   Plasmid

   #149557

   Purpose

   gRNA expression in B. burgdorferi, parental vector

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Psyn and pSyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-sgRNA_Drosha_4

   Plasmid

   #79863

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pMST665_BB3_L 23_gRNAempty_cas9_BbsI

   Plasmid

   #90278

   Purpose

   BB3_L 23_gRNAempty_cas9_BbsI; CRISPR plasmid with empty gRNA spacer to incorporate gRNA, Cas9

   Depositor

   Michael Sauer

   Article

   Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

   Insert

   Cas9
   

   Use

   A. niger

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330-sgRNA_Drosha_1

   Plasmid

   #79860

   Purpose

   CRISPR/Cas9 DROSHA

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   sgRNA Drosha
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

10. pX330-sgRNA_Drosha_2

    Plasmid

    #79861

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pFETCh_ZNF331

    Plasmid

    #135726

    Purpose

    Donor vector for 3' FLAG tag of human ZNF331

    Depositor

    Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF331 Homology arms (ZNF331 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330-sgRNA_Drosha_3

    Plasmid

    #79862

    Purpose

    CRISPR/Cas9 DROSHA

    Depositor

    Constance Ciaudo

    Article

    Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

    Insert

    sgRNA Drosha
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

13. pX330.iGFP5

    Plasmid

    #66584

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pX330.LoxPO

    Plasmid

    #66585

    Purpose

    sgRNA for LSL

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    LSL-Tomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pENTR-R4R3-mEGFP-N

    Plasmid

    #113746

    Purpose

    Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFETCh_ZNF580

    Plasmid

    #86278

    Purpose

    Donor vector for 3' FLAG tag of human ZNF580

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ZNF580 homology arms (ZNF580 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFETCh_SAP130

    Plasmid

    #86267

    Purpose

    Donor vector for 3' FLAG tag of human SAP130

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    SAP130 homology arms (SAP130 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR-R4R3-mEGFP-C

    Plasmid

    #113749

    Purpose

    Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mEGFP
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-R4R3-mRuby-C

    Plasmid

    #113755

    Purpose

    Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-R4R3-3xmRuby-C

    Plasmid

    #113757

    Purpose

    Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    mRuby2-mRuby2-mRuby2
    

    Use

    CRISPR; Gateway entry vector

    Tags

    3xmRuby2

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX330.iGFP3

    Plasmid

    #66583

    Purpose

    sgRNA for iGFP

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    iGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pGEL678

    Plasmid

    #207511

    Purpose

    GmUbi3-SpRY-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GmUBI3

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEL679

    Plasmid

    #207512

    Purpose

    GmM4-SpRY-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GmM4

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEL680

    Plasmid

    #207513

    Purpose

    GmM4-SpRY CBE-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GmM4

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEL681

    Plasmid

    #207514

    Purpose

    GmM4-SpRY ABE-STU

    Depositor

    Yong Zhang

    Article

    Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    GmM4

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

    Plasmid

    #99696

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

    Depositor

    George Church

    Insert

    dCas9 and gRNA targeting Afp (Afp Synthetic)
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMRS-BG35-GFP

    Plasmid

    #220201

    Purpose

    mini-Tn7 GFP expression vector - pBG35 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    monomeric superfolder green fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG35

    Available Since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSpyA-GFP

    Plasmid

    #220189

    Purpose

    sgRNA expression vector - pEM7 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pEM7 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pEM7

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSpyD-GFP

    Plasmid

    #220192

    Purpose

    sgRNA expression vector - pBG28 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG28 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG28

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dSp-Rta Full (1-190)

    Plasmid

    #99662

    Purpose

    Expresses dSp Cas9 fused to truncated RTA activation domain

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    Tags

    truncated RTA activation domain (1-190)

    Expression

    Mammalian

    Mutation

    dead Cas9

    Promoter

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

    Plasmid

    #196843

    Purpose

    Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSECRETS-B

    Plasmid

    #196987

    Purpose

    For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.

    Depositor

    Eric Josephs

    Article

    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1

    Insert

    Spy gRNA cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pltetO1

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. qTAG-C-sTagRFP-Zeo

    Plasmid

    #207734

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. qTAG-N-Zeo-sTagRFP

    Plasmid

    #207701

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. qTAG-C-sTagRFP-Blast

    Plasmid

    #207712

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. qTAG-N-Puro-sTagRFP

    Plasmid

    #207690

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. qTAG-N-Blast-sTagRFP

    Plasmid

    #207679

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pJAT58

    Plasmid

    #204304

    Purpose

    Expresses jGCaMP7s-T2A-tdTomato under UAS control. HDR event marked with removable 3XP3-DsRed cassette expressed in eyes.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    3XP3-DsRed
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    3XP3

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDGE1176

    Plasmid

    #202774

    Purpose

    CRISPR-KO in Arabidopsis thaliana

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

    Inserts

    FAST marker
    pRPS5a:zCas9_tNbEU
    ccdB cassette (BsaI-excisable)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMLS538

    Plasmid

    #188881

    Purpose

    Gateway Entry Vector [1-2]: 2xNLS::Cas9 (with 4 introns from smu-2)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

    Insert

    Cas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    C. elegans codom optimization and intron addition

    Promoter

    Available Since

    Aug. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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46. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pM159

    Plasmid

    #173215

    Purpose

    Expresses dCas9-sfGFP10

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    dCas9-sfGFP10M2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pORANGE Tubb3-2xGFP KI

    Plasmid

    #183443

    Purpose

    GFP knock-in for beta3-tubulin-GFP (amino acid position: stop codon)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and 2xGFP donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pOC4 Kcnn2-smFP-myc KI

    Plasmid

    #183438

    Purpose

    FlpON knock-in for SK2-smFP-myc (amino acid position: T571)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP myc donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge