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159,258 results

Showing: 2761 - 2780 of 159258 results

  3. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Article
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Use
    Tags
    Expression
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

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  4. pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025)

    Plasmid
    #140003
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY with P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140003-plasmid-map-sequence-id-337354

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  7. AICSDP-8:TOMM20-mEGFP

    Plasmid
    #87423
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TOMM20
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Insert
    TOMM20 Homology Arms with linker-mEGFP (TOMM20 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    87423-plasmid-map-sequence-id-173982

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  9. AAV-sCAG-mBaoJin-oPRE

    Plasmid
    #218684
    Purpose
    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.
    Depositor
    Bart Nieuwenhuis
    Insert
    mBaoJin
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Short variant of the CAG promoter
    Available since
    July 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    218684-plasmid-map-sequence-id-438234

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  11. pBiFC-VC155

    Plasmid
    #22011
    Purpose
    Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays
    Depositor
    Chang-Deng Hu
    Article
    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.
    Insert
    VC155
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Alanine 206 to Lysine
    Promoter
    Available since
    Sept. 28, 2009
    Availability
    Academic Institutions and Nonprofits only
    22011-plasmid-map-sequence-id-354492

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  12. TBG-Vector

    Plasmid
    #109396
    Purpose
    AAV-CRISPR knockout plasmid with Cre recombinase driven by TBG promoter for direct in vivo genome editing in mouse liver
    Depositor
    Sidi Chen
    Article
    Wang et al Sci Adv. 2018 Feb 28;4(2):eaao5508. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    109396-plasmid-map-sequence-id-222776

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  16. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    160729-plasmid-map-sequence-id-322926

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  20. EGFP-pBAD

    Plasmid
    #54762
    Purpose
    Localization: Protein Expression Vector , Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Tags
    6xHis and EGFP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54762-plasmid-map-sequence-id-212808

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Showing: 2761 - 2780 of 159258 results