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Showing: 651 - 700 of 17678 results

  8. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Use
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  10. SBC015910

    Plasmid
    #226287
    Purpose
    Expresses PhCFAT and PhIGS1 from trc promoter. Biosynthesis of isoeugenol from coniferyl alcohol.
    Depositor
    Rainer Breitling
    Article
    Chua et al Metab Eng Commun. 2024 May 15;18:e00237.
    Inserts
    Coniferyl alcohol acyltransferase
    Isoeugenol synthase 1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226287-plasmid-map-sequence-id-451913

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  11. pRSF-ChPylTMSK

    Plasmid
    #163915
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163915-plasmid-map-sequence-id-327916

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  14. pAS

    Plasmid
    #140008
    Purpose
    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021
    Insert
    4xPylT (Mma) cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    U25C
    Promoter
    h7SK
    Available since
    May 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    140008-plasmid-map-sequence-id-296092

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  17. MT2a_K30A

    Plasmid
    #105694
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 30 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105694-plasmid-map-sequence-id-203312

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  18. MT2a_K31A

    Plasmid
    #105695
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 31 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105695-plasmid-map-sequence-id-203313

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  20. MT2a_C21A

    Plasmid
    #105697
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Cysteine 21 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105697-plasmid-map-sequence-id-203314

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  21. MT2a_K20A

    Plasmid
    #105698
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Lysine 20 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105698-plasmid-map-sequence-id-203315

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  22. MT2a_S6A

    Plasmid
    #105699
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    Metallothionein 2a (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Serine 6 to Alanine
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105699-plasmid-map-sequence-id-203317

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  25. pAWPSG-Po-nCas9-BE

    Plasmid
    #195740
    Purpose
    Express APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coli
    Depositor
    Seung-Goo Lee
    Article
    Jeong et al Chem Eng J, 2023
    Insert
    Di-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Po (phoenol-inducible promoter)
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195740-plasmid-map-sequence-id-386428

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  27. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172316
    Purpose
    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172316-plasmid-map-sequence-id-343650

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  28. MT2A_beta-Villin

    Plasmid
    #200301
    Purpose
    Bacterial expression plasmids for production of recombinant fusion protein having beta-domain of metallothionein 2a and chicken Villin headpiece domain HP-35
    Depositor
    Artur Krężel
    Article
    Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d
    Insert
    beta-domain of metallothionein 2a in fusion with HP-35 (MT2A Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    200301-plasmid-map-sequence-id-394918

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  30. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Article
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Use
    Tags
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61628-plasmid-map-sequence-id-396550

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  33. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

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  35. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Use
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  36. pMega-MaFRSA

    Plasmid
    #200226
    Purpose
    The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..
    Depositor
    Alanna Schepartz
    Article
    Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155
    Inserts
    Pyrrolysyl-tRNA synthetase
    pyrrolysyl-tRNA
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    mutated Asparagine 166 to Alanine and Valine 168 …
    Promoter
    proK-lacO and tacI
    Available since
    June 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    200226-plasmid-map-sequence-id-393639

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  40. pCDF-5xT7-TtCsm

    Plasmid
    #128572
    Purpose
    For expression of T. thermophilus Csm in E. coli (Csm1-5)
    Depositor
    Jennifer Doudna
    Article
    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:
    Inserts
    Csm1
    Csm2, Csm3, Csm4, Csm5
    Use
    Tags
    HRV 3C protease cleavage site and 10xHis tag on C…
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli and Cod…
    Promoter
    T7 promoter, lac operator
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128572-plasmid-map-sequence-id-252577

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  42. pBa-tdTomato-flag-BicD2 594-FKBP

    Plasmid
    #64205
    Purpose
    Bicaudal D protein aa1-594 (excluding start Met) with N-terminal tandem dimer Tomato and flag tags and C-terminal FKBP
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Insert
    Bicaudal D protein (Bicd2 Mouse)
    Use
    Tags
    FKBP, flag, and tdTomato
    Expression
    Mammalian
    Mutation
    no start methionine, aa1-594
    Promoter
    chicken Beta Actin
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    64205-plasmid-map-sequence-id-107603

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  43. pBa-eGFP-flag-BicD2 594-FKBP

    Plasmid
    #63569
    Purpose
    Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP
    Depositor
    Gary Banker, Marvin Bentley
    Article
    Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.
    Insert
    Bicaudal D protein (Bicd2 Mouse)
    Use
    Tags
    FKBP, eGFP, and flag
    Expression
    Mammalian
    Mutation
    no start methionine, aa1-594
    Promoter
    chicken Beta Actin
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63569-plasmid-map-sequence-id-105705

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  46. cfmTurquoise-N

    Plasmid
    #139284
    Purpose
    Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.
    Depositor
    Ikuo Wada
    Article
    Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d
    Insert
    cfmTurquoise
    Use
    Tags
    Multi-cloning sites
    Expression
    Mammalian
    Mutation
    Cys48Ser and Cys70/Met
    Promoter
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    139284-plasmid-map-sequence-id-291944

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Showing: 651 - 700 of 17678 results