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We narrowed to 14,145 results for: TIM

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Showing: 651 - 700 of 14145 results

  14. pH2B-SCFP1-2A-mTq2-NES

    Plasmid
    #60491
    Purpose
    Co-expresses two cyan fluorescent protein variants, a short fluorescence lifetime CFP variant in the nucleus and a CFP variant with a long lifetime (mTurquoise2) in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    H2B-SCFP1
    mTurquoise2-NES
    Tags
    SCFP1 and mTurquoise2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60491-plasmid-map-sequence-id-96973

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  18. TRE-pfv-Sapphire

    Plasmid
    #231957
    Purpose
    The plasmid containing TRE-pfv-Sapphire for the doxycycline-inducible expression of genetically encoded multimeric nanoparticles (GEM)
    Depositor
    Michael Shtutman
    Article
    Korunova et al bioRxiv [Preprint]. 2024 Nov 18:2024.11.
    Insert
    genetically encoded multimeric nanoparticles
    Use
    Lentiviral; Doxocycline inducible
    Expression
    Mammalian
    Promoter
    tight TRE promoter
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    231957-plasmid-map-sequence-id-462153

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  26. 3xNLS-SGFP2(T65G)-T2A-SGFP2-3xNES

    Plasmid
    #60492
    Purpose
    Co-expresses two green fluorescent protein variants, a short fluorescence lifetime GFP variant in the nucleus and a GFP variant with a long lifetime in the cytoplasm
    Depositor
    Dorus Gadella
    Article
    Joosen et al J Microsc. 2014 Sep 1. doi: 10.1111/jmi.
    Inserts
    3xnls-SGFP2(T65G)
    SGFP2-3xNES
    Tags
    SGFP2 and SGFP2(T65G)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    60492-plasmid-map-sequence-id-96974

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  30. pNB784

    Plasmid
    #60163
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60163-plasmid-map-sequence-id-96372

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  31. pNB788

    Plasmid
    #60165
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60165-plasmid-map-sequence-id-96374

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  33. pNB786

    Plasmid
    #60151
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60151-plasmid-map-sequence-id-96314

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  35. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Promoter
    Trc (lactose/IPTG inducible)
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45940-plasmid-map-sequence-id-64653

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  37. pBHM2403

    Plasmid
    #171687
    Purpose
    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.
    Depositor
    Hiten Madhani
    Article
    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi
    Insert
    CnoCAS9
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Promoter
    TEF1
    Available Since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    171687-plasmid-map-sequence-id-340174

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  39. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Joachim Wittbrodt
    Article
    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Promoter
    sp6
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    186929-plasmid-map-sequence-id-390733

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  42. JBEI-3716

    Plasmid
    #100169
    Purpose
    Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth
    Depositor
    Jay Keasling
    Article
    Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.
    Insert
    codon-optimized MK
    Expression
    Bacterial
    Available Since
    Sept. 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    100169-plasmid-map-sequence-id-193493

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  43. pNB785

    Plasmid
    #60164
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    SIC1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60164-plasmid-map-sequence-id-96373

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  44. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  45. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  46. pNB682

    Plasmid
    #60143
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    A4V
    Promoter
    MET17pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60143-plasmid-map-sequence-id-96214

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  49. pLT 651

    Plasmid
    #59998
    Purpose
    Expresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progeny
    Depositor
    Lisa Timmons
    Article
    Timmons et al FEBS Lett. 2014 Jul 24. pii: S0014-5793(
    Inserts
    klp-16 (klp-16 Nematode)
    him-8 (him-8 Nematode)
    Expression
    Bacterial
    Mutation
    partial genomic
    Available Since
    April 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    59998-plasmid-map-sequence-id-110771

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Showing: 651 - 700 of 14145 results