12,254 results

1. pBbA13a-RFP

   Plasmid

   #126086

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23102

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-EsaR-D91G

   Plasmid

   #47646

   Purpose

   pAC-EsaR with a a272g mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-D91G
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Changed Aspartic acid 91 to Glycine

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pYZ714

   Plasmid

   #132955

   Purpose

   crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with dFnCas12a-Clr4 in Schizosaccharomyces pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al Nucleic Acids Res. 2020 Jun 4;48(10):578

   Insert

   dFnCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   SV40 NLS and nucleoplasmin NLS

   Promoter

   adh1

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-miR-7-5p

   Plasmid

   #103723

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-7-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-7-5p target
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOLENTE

   Plasmid

   #80683

   Purpose

   DEST vector for cell-free in vitro expression

   Depositor

   Nico Dissmeyer

   Type

   Empty backbone

   Use

   Synthetic Biology; In vitro transcription and tra…

   Expression

   Mammalian

   Promoter

   T7 and SP6

   Available Since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACBB-eGFP

   Plasmid

   #32551

   Depositor

   Claudia Schmidt-dannert

   Article

   Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

   Insert

   eGFP (eGFP )
   

   Use

   Synthetic Biology

   Tags

   His

   Promoter

   Consitutive lac

   Available Since

   Nov. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTn7xTS-mPlum

   Plasmid

   #117392

   Purpose

   Tn7 tagging vector pTn7xTS with mPlum expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   mPlum
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pES134

   Plasmid

   #127551

   Purpose

   Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

   Depositor

   Gavin Thomas, Emmanuele Severi

   Article

   Wu et al Microbiology (Reading). 2024 Mar;170(3).

   Insert

   F3-flanked spectinomycin resistance cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTK615

    Plasmid

    #110609

    Purpose

    BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    sgRNA
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellulose

    Plasmid

    #36300

    Depositor

    Jay Keasling

    Article

    Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

    Insert

    pCellulose
    

    Use

    Synthetic Biology

    Promoter

    Two promoters from the E coli genome drive two di…

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP782

    Plasmid

    #98790

    Purpose

    Accessory Plasmid for PACE

    Depositor

    David Liu

    Article

    Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

    Insert

    proB-lysozyme-ggs-HPLVGHM-ggs-T7RNAP//T7pro-gIII-lux
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBeast-pAhpC-RBS- LacZ

    Plasmid

    #190060

    Purpose

    LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

    Depositor

    Jerome Bonnet

    Article

    Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

    Insert

    lacZ
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC-EsaR-EsaI

    Plasmid

    #47660

    Purpose

    esaI gene downstream of esaR in pAC-EsaR (CmR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    esaI-term
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pdCas9-M-G6

    Plasmid

    #73437

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor G6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available Since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

    Plasmid

    #178989

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK1056

    Plasmid

    #209546

    Purpose

    BTK Type 4 antibiotic resistance cassette for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Type 4 Kanamycin resistance cassette
    

    Use

    Synthetic Biology

    Available Since

    Jan. 12, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pL0_EF-PtBle

    Plasmid

    #202180

    Purpose

    Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Insert

    ShBle expression cassette
    

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pBbB7a-GFP

    Plasmid

    #35358

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH03C_32

    Plasmid

    #120897

    Purpose

    Functional test of ECF sigma factors and their cognate promoters

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

    Insert

    ECF32_1122
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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