We narrowed to 24,591 results for: p

1. P3-dCas9-Tet1-GFP-Puro

   Plasmid

   #190728

   Purpose

   Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

   Depositor

   Maja Jagodic

   Article

   Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

   Insert

   dCas9-tet1
   

   Use

   CRISPR

   Tags

   3XFLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   Changed Pro434 codon from CCG to CCC and Gly468 f…

   Promoter

   pCAG

   Available Since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgTP53_3

   Plasmid

   #78164

   Purpose

   sgTP53

   Depositor

   William Hahn

   Article

   Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3.1 Flag-HIF1B (#930)

   Plasmid

   #99916

   Purpose

   mammalian expression

   Depositor

   James Brugarolas

   Article

   Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

   Insert

   HIF1B (ARNT Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Nov. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE

   Plasmid

   #51509

   Purpose

   Can be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoter

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Has Service

   AAV1

   Insert

   tdTomato-T2A-SypEGFP
   

   Use

   AAV

   Promoter

   phSyn1

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-N1 hMOG-beta1-EGFP

   Plasmid

   #160978

   Purpose

   Expresses a human myelin oligodendrocyte glycoprotein isoform beta1 - EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

   Insert

   Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant beta1 (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-TET-MKOS

   Plasmid

   #20959

   Purpose

   piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Available Since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_hSyn-PdCO-EGFP-WPRE

   Plasmid

   #198513

   Purpose

   Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Has Service

   AAV1 and AAV5

   Insert

   PdCO
   

   Use

   AAV

   Tags

   EGFP and Rho1D4

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-SIO-PdCO-mScarlet-WPRE

   Plasmid

   #198509

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

   Plasmid

   #198515

   Purpose

   Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

   Depositor

   Ofer Yizhar

   Article

   Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

   Insert

   PdCO
   

   Use

   AAV and Cre/Lox

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-7xTRE-3xeGFP

    Plasmid

    #191206

    Purpose

    GFP expression under the control of TRE promotor

    Depositor

    Janelia Viral Tools

    Article

    Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

    Insert

    3X GFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-mTubb3

    Plasmid

    #87116

    Purpose

    AAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    U6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
    

    Use

    AAV and CRISPR

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAi14-luc-MC

    Plasmid

    #87113

    Purpose

    minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    Luciferase-SV40pA
    

    Use

    CRISPR and Luciferase

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLED.NPL

    Plasmid

    #193014

    Purpose

    Neuron-specific reporter (CBh promoter, Pls3 exon, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-1BPNLS-Cas9-1BPNLS

    Plasmid

    #87108

    Purpose

    BPNLS-Cas9-BPNLS expression plasmid

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    1BPNLS-Cas9-1BPNLS
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE

    Plasmid

    #198508

    Purpose

    Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    PdCO
    

    Use

    AAV

    Tags

    Rho1D4 and mScarlet

    Expression

    Mammalian

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAi14-GFPNLS-MC

    Plasmid

    #87114

    Purpose

    minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

    Depositor

    Juan Belmonte

    Article

    Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

    Insert

    GFPNLSPpA
    

    Use

    CRISPR

    Promoter

    nEF

    Available Since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLXI_TRC403 LacZ

    Plasmid

    #111184

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLXI_TRC401 LacZ

    Plasmid

    #111183

    Purpose

    Control inducible vector with LacZ (V5 tagged)

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    lacZ
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    TRE

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLED.GluA2

    Plasmid

    #193017

    Purpose

    GluA2 (Gria2) flip/flop reporter (hSyn promoter, Gria2 flip/flop exons, bichromatic reporter)

    Depositor

    Jonathan Ling

    Article

    Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

    Insert

    Bichromatic reporter (EGFP and dsRed-Express2)
    

    Use

    AAV

    Available Since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1 hMOG-alpha2-EGFP

    Plasmid

    #160976

    Purpose

    Expresses a human myelin oligodendrocyte glycoprotein isoform alpha2 - EGFP fusion protein in mammalian cells

    Depositor

    Markus Reindl

    Article

    Schanda et al Neurol Neuroimmunol Neuroinflamm. 2021 J

    Insert

    Homo sapiens myelin oligodendrocyte glycoprotein (MOG), transcript variant alpha2 (MOG Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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