161,570 results

1. BRD4-N CRISPR

   Plasmid

   #140651

   Purpose

   BRD4 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   BRD4 (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CL20_mEGFP_YAP1_MAMLD1

   Plasmid

   #205955

   Purpose

   Express mEGFP-tagged fusion protein, YAP1_MAMLD1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   YAP1_MAMLD1 (YAP1, MAMLD1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKSV7

   Plasmid

   #26686

   Depositor

   Philip Youngman

   Article

   Smith et al Biochimie. . 74(7-8):705-11.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. lenti-sgRNA blast

   Plasmid

   #104993

   Purpose

   This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRF-SrpR

   Plasmid

   #49372

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   SrpR repressor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. mTLR9

   Plasmid

   #13091

   Depositor

   Ruslan Medzhitov

   Insert

   TLR9 (Tlr9 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-21a(+)/MBP-His-PINK1

   Plasmid

   #38005

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Tags

   6xHIS and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pET28a_MBP_RBFOX2

   Plasmid

   #224292

   Purpose

   MBP-RBFOX2 protein purification from bacteria

   Depositor

   Chuan He

   Article

   Dou et al Nat Cell Biol. 2023 Sep;25(9):1359-1368.

   Insert

   RNA binding fox-1 homolog 2 (RBFOX2 Human)
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUFlip-floxed2A-mRFP-; HE1A:BFP -1

   Plasmid

   #180008

   Purpose

   Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

   Depositor

   Jeffrey Essner

   Article

   Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

   Insert

   2A-mRFP; HE1A: BFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBSV2B

    Plasmid

    #118228

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector, blasticidin S resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GABA BR2-flag/pRK5

    Plasmid

    #197323

    Purpose

    Plasmid expressing an antigen targeting the GABA(B)R2 metabotropic GABA receptor

    Depositor

    Stephen Moss

    Insert

    GABA B R2 (Gabbr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GPX8 rescue PLAK1

    Plasmid

    #161517

    Purpose

    Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

    Depositor

    Yoav Shaul

    Article

    Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

    Insert

    glutathione peroxidase 8 (GPX8 Human)
    

    Use

    Lentiviral

    Tags

    3X Flag

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pmCherry-C1_SEPT9 i1

    Plasmid

    #71622

    Purpose

    mammalian expression of human SEPT9 with an mCherry fusion

    Depositor

    Elias Spiliotis

    Article

    Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1.Gαi2-LgB91

    Plasmid

    #134358

    Purpose

    Nanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi2-LgB91 (GNAI2 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MAC_C_CH60

    Plasmid

    #111662

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CH60 (HSPD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Ube1/PET21d

    Plasmid

    #34965

    Depositor

    Cynthia Wolberger

    Article

    Berndsen et al Anal Biochem. 2011 Nov 1;418(1):102-10.

    Insert

    Ube1 (UBA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUFlip-floxed2A-mRFP-; gcry1:BFP -2

    Plasmid

    #173888

    Purpose

    Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

    Insert

    2A-mRFP; gcry1: BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-U6-sgRNA-BFP

    Plasmid

    #107722

    Purpose

    For expression of sgRNA from the U6 promoter with BFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-anti-VEGF(Nb35) PAGER(TF)

    Plasmid

    #230000

    Purpose

    Expresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    IL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    V360A/R361A on KORD

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1.Gα12-LgB115

    Plasmid

    #134363

    Purpose

    Nanoluc complementation assay. Expression of Gα12 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 115 and 116 of Gα12.Addition of the HA epitope at N terminus of Gα12.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gα12-LgB115 (GNA12 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge