163,606 results

1. pCWXPGR-pTF-betaCatenin

   Plasmid

   #114281

   Purpose

   TET-inducible expression of a constitutively active form of the BetaCatenin protein

   Depositor

   Patrick Salmon

   Article

   Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

   Insert

   Catenin beta 1 (CTNNB1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   deletion of AA 1 to 45

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT281

   Plasmid

   #122611

   Purpose

   express evoAPOBEC1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoAPOBEC1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E4K H109N H122L D124N R154H A165S P201S F205S

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet30b-7M SrtA

   Plasmid

   #51141

   Purpose

   expression plasmid for C-terminal his tagged, calcium independent S.aureus SrtA with enhanced catalytic activity

   Depositor

   Hidde Ploegh

   Insert

   S.aureus SrtA 7M
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, E105K, E108Q, D160N, D165A, K190E, K196T

   Promoter

   T7

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDestTol2pA2-9xGRE

   Plasmid

   #74736

   Purpose

   GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

   Depositor

   Luisa Dalla Valle

   Article

   Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

   Insert

   EGFP
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   nine GC-responsive elements (9xGRE) from the rat …

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-FLEX-tdTomato

   Plasmid

   #28306

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPlatTET-gRNA2

   Plasmid

   #82559

   Purpose

   All in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.

   Depositor

   Izuho Hatada

   Article

   Morita et al Nat Biotechnol. 2016 Aug 29. doi: 10.103

   Insert

   dCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRRL-mU6-mScarletGO-P2A-Blas-PGK-Neo

   Plasmid

   #136904

   Purpose

   Lentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   mScarletGo-p2a-Blas
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC18R6KT-mini-Tn7T-Km

   Plasmid

   #64969

   Purpose

   mini-Tn7 base vector with transcriptional terminator and oriT

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. ER-mNeonGreen

    Plasmid

    #137804

    Purpose

    Visualization of the Endoplasmic Reticulum

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FSW_jGCaMP8f

    Plasmid

    #197034

    Purpose

    Express GCaMP8f from hSyn1 promoter

    Depositor

    Ronald Hart

    Insert

    GCaMP8f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. FUW

    Plasmid

    #14882

    Depositor

    David Baltimore

    Article

    Lois et al Science. 2002 Feb 1. 295(5556):868-72.

    Insert

    flap-Ub promoter-WRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVM_02

    Plasmid

    #204500

    Purpose

    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)

    Depositor

    Alessio Ciulli

    Article

    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.

    Insert

    amino acids 54-213 VHL (VHL Human)
    

    Use

    Tags

    6xHis, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    0

    Promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXR001: EF1a-CasRx-2A-EGFP

    Plasmid

    #109049

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Insert

    CasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    2A-eGFP, HA, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AbVec2.0-IGHG1

    Plasmid

    #80795

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

    Depositor

    Hedda Wardemann

    Article

    Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. hCathepsin L

    Plasmid

    #11250

    Purpose

    Mammalian expression of human cathepsin L

    Depositor

    Hyeryun Choe

    Article

    Huang et al J Biol Chem. 2006 Feb 10;281(6):3198-203

    Has Service

    Cloning Grade DNA

    Insert

    cathepsin L (CTSL Human)
    

    Use

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-E-Syt2

    Plasmid

    #66831

    Purpose

    Mammalian Expression, Bacterial Expression

    Depositor

    Pietro De Camilli

    Article

    Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

    Insert

    E-Syt2 (ESYT2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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