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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Article

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-NanoLuc

Plasmid

#188116

Purpose

Template plasmid to transcribe circular RNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMCP-Luc (MCP-1 promoter in pGL3-basic)

Plasmid

#40324

Depositor

Alexander Dent

Article

Toney et al Nat Immunol. 2000 Sep;1(3):214-20.

Insert

MCP-1 promoter (Ccl2 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

contains MCP-1 promoter region through the nucleo…

Promoter

MCP-1

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pKL2299A

Plasmid

#222105

Purpose

A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

Depositor

Kan Wang

Article

Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

Insert

virA

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

virA promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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ClopHensorN

Plasmid

#50758

Purpose

Mammalian expression vector expressing the ClopHensorN chloride and proton sensor

Depositor

Colin Akerman

Article

Raimondo et al Front Cell Neurosci. 2013 Nov 13;7:202.

Insert

ClopHensorN

Use

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Promoter

CAG

Available since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

Plasmid

#124429

Purpose

Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(TEV cleavage seq) T2A mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLDLR-Luc (aka: pES7)

Plasmid

#14940

Depositor

Axel Nohturfft

Article

Castoreno et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

LDLR promoter (LDLR Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pCMT-flp

Plasmid

#67274

Purpose

Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

Depositor

Mark Liles

Article

Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

Insert

tetA, flp, repA101, oriR101

Use

Flp recombinase plasmid, temperature sensitive

Tags

His-SUMO (flp)

Expression

Mutation

Promoter

tetA promoter of plasmid pACYC184 origin

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4-VP16

Plasmid

#71728

Purpose

plasmid expressing fusion construct of Gal4 DBD and VP16 AD

Depositor

Lea Sistonen

Article

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

Gal4 DBD(1-147) (GAL4 Budding Yeast)

Use

Tags

VP16 AD (413-490)

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pET-His-Cre

Plasmid

#123128

Purpose

Bacterial expression plasmid for wild-type Cre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Frb-EGFP-HOTag6

Plasmid

#106919

Purpose

Express Frb-EGFP-HOTag6 fusion in mammalian cells.

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

Frb-EGFP-HOTag6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mito-dendra2

Plasmid

#55796

Purpose

photo-activatable mitochondrial marker for expression in mammalian cells

Depositor

David Chan

Article

Pham et al Genesis. 2012 Nov;50(11):833-43. doi: 10

Insert

mito-Dendra2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pRSETB/mStayGold(c4)

Plasmid

#212018

Purpose

For N-terminal tagging with mStayGold. c4 is the appendage that facilitates fusions to the C-terminus of mStayGold.

Depositor

Atsushi Miyawaki

Article

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

mStayGold(c4)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag Pu.1

Plasmid

#66974

Purpose

Expression plasmid for mouse Pu.1; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Article

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

Spi1 (Spi1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only
p5E-3x125bp-mnx1

Plasmid

#169043

Purpose

5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

Mnx1 enhancer (3x) (mnx1 Zebrafish)

Use

Gateway entry cloning vector

Tags

Expression

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-UbC-FKRP-EF1a-BSD

Plasmid

#205150

Purpose

Express UbC-driven FKRP and EF-1a driven BSD

Depositor

Monkol Lek

Insert

Fukutin-related protein (FKRP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS2

Plasmid

#185137

Purpose

non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS2 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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