We narrowed to 5,634 results for: pcas
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Plasmid#227299PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of TJP1 (TJP1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-EA-BE4max-NG-BlastR
Plasmid#152992PurposeHigh purity C-to-T base editorDepositorInsertBE4 H47E+S48A-nSpCas9-UGI-UGI
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBECKP-km
Plasmid#117235PurposeA cytidine deaminase-mediated base-editing plasmid in Klebsiella PneumoniaeDepositorInsertncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP
Plasmid#32476PurposeExpresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP markerDepositorInsertPSAM Y115F,L141F-5HT3 HC
UseTagsIRES-GFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorInsertAMPK alpha 1 (PRKAA1 Human)
UseTagsExpressionMammalianMutationPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32474PurposeExpresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP markerDepositorInsertPSAMQ79G,Q139G-5HT3 HC
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
TrkB Acceptor
Plasmid#83953PurposeFRET acceptor for TrkB mEGFPDepositorInsertPhospholipase C gamma 1 (Plcg1 Mouse)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP115E
Plasmid#97401Purposebinary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsGolgi targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP116E
Plasmid#97402Purposebinary construct of plasma membrane-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsPlasma membrane targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lys-eMagB-iRFP
Plasmid#162249PurposeBlue-light dependent heterodimerization systemDepositorInsertp18-eMagB-iRFP
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Gg
Plasmid#61653PurposeGateway vector for generating green fluorescent protein fusions in fungiDepositorInsertenhanced green fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PE-P3-rice
Plasmid#185612PurposeFor plant prime editing in rice plantsDepositorInsert3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS-P2A-Hyg
UseCRISPRTagsExpressionMutationPromoterZmUbiAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC016-gPsp-EGFP_start_codon
Plasmid#233613PurposeExpression of guide RNA targeting the start codon of the EGFP gene for PspCas13bDepositorInsertgRNA targeting EGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
ER-EGFP-eMagA
Plasmid#162245PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-EGFP-eMagA
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagB-iRFP-Mito
Plasmid#162250PurposeBlue-light dependent heterodimerization systemDepositorInserteMagB-iRFP-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailable sinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorInsertCodon optimized Cas9
UseCRISPRTags3x FLAGExpressionMutationSilent change from C to A at bp 834 of insertPromoterAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
ER-mCherry-eMagA
Plasmid#162248PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-mCherry-eMagA
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GB4080
Plasmid#215249PurposeModule for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.DepositorInsertSF-EGFP-p19-LUZ-H3H-HISPS-CPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTalpha1-iCre
Plasmid#133924PurposeNeuron-specific expression of iCreDepositorInsertiCre
UseTagsExpressionMammalianMutationCodon optimizedPromoterneuron-specific tubulin-alpha-1 promoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
eMagA-mCherry-Mito
Plasmid#162251PurposeBlue-light dependent heterodimerization systemDepositorInserteMagA-mCherry-OMP25
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_Blasto
Plasmid#167856PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJ1021
Plasmid#228761PurposePlasmid expressing Cas9 and 2 gRNAs for mouse Ift88. Use for disruption of mouse Ift88 in cultured cells.DepositorInsertIft88 gRNAs (Ift88 Mouse)
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterhU6Available sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
XLone-Cas9-2A-GFP
Plasmid#170159PurposeExpress Cas9 and GFP upon doxycycline treatmentDepositorInsertCas9
UseSynthetic Biology; PiggybacTagsGFPExpressionMammalianMutationPromoterTRE3G promoterAvailable sinceJuly 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIP-caTfeb
Plasmid#79013PurposeFor mammalian expression of a shortened, constitutively active version of Tfeb.DepositorInsertTfeb (TFEB Human)
UseTagsExpressionMammalianMutationRemoved first ~400 bp from 5' end (N-terminu…PromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRU319
Plasmid#167686PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::spCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSQT834
Plasmid#53371PurposeCsy4 and Cas9 nuclease expression plasmidDepositorInsertCsy4-T2A-Cas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_221
Plasmid#91115PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only