We narrowed to 9,474 results for: control
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Plasmid#103801PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only
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pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract
Plasmid#138525PurposeExpresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.DepositorInsertControl non-PRC2 binding A-tract repeat RNA
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 HGV-mRuby3
Plasmid#179227Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
TagsmNeongreen and mRuby3ExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
B4GALT1-Dox
Plasmid#124633PurposeDoxycycline-inducible B4GALT1 expression in mammalian cellsDepositorInsertsBeta-(1,4)-Galactosyltransferase 1
Tetracycline repressor A3 mutant
TagFP635
ExpressionMammalianPromoterTRE-tight and hEF1aAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-4A6
Plasmid#73434PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 4A6.DepositorInsertRepressor 4A6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-INPP5E(D556A)
Plasmid#79563Purposeexpression of mCherry-tagged CRY2PHR fused to catalytically dead INPP5EDepositorInsertINPP5E (INPP5E Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationC-terminal region, aa 214_644, C641A mutation to …PromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-OptoFGFR1-Y766F
Plasmid#59777PurposeExpresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1DepositorAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-LacI-tagRFP-T
Plasmid#103810Purpose'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterCMVAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
RRL.sin.cPPT.SFFV/Ace2.WPRE (MT136)
Plasmid#145842PurposeLentiviral vector genome to ectopically express human Ace2DepositorAvailable SinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNL3.1-Erg
Plasmid#182706PurposeExpress Erg enhancer in mammalian cellsDepositorInsertEnhancer of Erythroblast transformation-specific-Related Gene (Erg Mouse)
UseLuciferaseAvailable SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1.1-mCherry
Plasmid#162772PurposeFluorescent reporter for CHO expression studiesDepositorInsertred fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
ExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hs-HRAS
Plasmid#52730Purposeretroviral expression of human HRASDepositorAvailable SinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTH734-2µ-RLuc/stopCFLuc
Plasmid#40609DepositorInsertsFirefly Luciferase (nonsense mutant)
Renilla luciferase
ExpressionYeastMutationAll codons of the original FLuc gene were exchang…PromoterADH1 and TDH3Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1β
Plasmid#103830PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianPromoterEF1a and hU6Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a C2AB
Plasmid#40051DepositorInserthSlp2-a C2AB (SYTL2 Human)
TagsEGFPExpressionMammalianMutationC2AB domains only (aa 597-910)PromoterCMV promoterAvailable SinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
mOrange-P2A-hKvb1
Plasmid#154084PurposeIndependent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoterDepositorInsertmOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
UseAAVExpressionMammalianPromoterhuman synapsin 1Available SinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a del.SHD
Plasmid#40050DepositorInserthSlp2-a del.SHD (SYTL2 Human)
TagsEGFPExpressionMammalianMutationdeletion of SHD domain (1-56)PromoterCMV promoterAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-C4
Plasmid#73427PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EglN2 wt-pBabe-Puro
Plasmid#22704DepositorInsertEgg laying Nine - 2 (EglN2) (EGLN2 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationwt cDNAAvailable SinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 mitoAURKA
Plasmid#157754PurposeExpression of AURKA:GFP targeted to mitochondriaDepositorInsertAURKA (AURKA Human)
TagsGFPExpressionMammalianMutationAURKA without the first 30 amino acids replaced b…PromoterCMVAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
Plasmid#51249PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 980,1006 mutated to AlaPromoterEF1aAvailable SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
gRNAs[bTub+Sxl].1046B
Plasmid#112692Purposeexpress two gRNA targeting bTub & Sxl under dU6-3 promoterDepositorInsertU6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
UseCRISPRAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a del.SHD
Plasmid#40036DepositorInserthSlp4-a del.SHD (SYTL4 Human)
TagsEGFPExpressionMammalianMutationdeleted SHD domain (1-143)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-BHLHE40(S243A/E248A)
Plasmid#110155PurposeExpression of human BHLHE40 (S243A/E248A) in mammalian cellsDepositorInsertBHLHE40 (basic helix-loop-helix family member e40) (BHLHE40 Human)
TagsHAExpressionMammalianMutationS243A/E248APromoterCMVAvailable SinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorAvailable SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-tdTomato-Raf1
Plasmid#104176PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1DepositorExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX-mPGK-CD90.2-Rluc_miR
Plasmid#163332PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the murine PGK promoter.DepositorAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3F2
Plasmid#73428PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.DepositorInsertRepressor C4 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGEX-DDX21-D2+C
Plasmid#179243Purposeprotein purification in E. coliDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
Plasmid#51247PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907 mutated to AlaPromoterEF1aAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα(D/D+linker)-EGFP
Plasmid#181842PurposeEGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.DepositorInsertRIα-(D/D+linker)EGFP (PRKAR1A Human)
TagsEGFPExpressionMammalianMutationContains only residues 12-112 of PKA RIα.PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMY-CD90.2-Rluc_miR
Plasmid#163333PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a CD90.2 surface marker under control of the LTR promoter.DepositorAvailable SinceJan. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 HGV
Plasmid#179230Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
TagsmNeongreenExpressionMammalianMutationD338H, E339GPromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
p23-mNeongreen-DDX21 DAT
Plasmid#179229Purposeoverexpression in human cellsDepositorInsertDDX21 (DDX21 Human)
TagsmNeongreenExpressionMammalianMutationG234D, K235APromoterEF-1alphaAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2AB
Plasmid#40037DepositorInserthSlp4-a C2AB (SYTL4 Human)
TagsEGFPExpressionMammalianMutationC2A and C2B domains (353-671)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a C2A
Plasmid#40038DepositorInserthSlp4-a C2A (SYTL4 Human)
TagsEGFPExpressionMammalianMutationC2A domain (353-487)PromoterCMV promoterAvailable SinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry-Raf1
Plasmid#104066PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1DepositorExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only