We narrowed to 13,038 results for: Mpl

1. HOXD4 (murine) HIS-tag pET

   Plasmid

   #8548

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by in vitro transcri…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pCDNA3-HA/Bmal L115E

   Plasmid

   #47345

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L115E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pEBTetD-SNAP-CP110

   Plasmid

   #136803

   Purpose

   Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

   Depositor

   Kai Johnsson

   Article

   Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

   Insert

   SNAP-CP110 (CCP110 Synthetic, Human)
   

   Use

   Tags

   FLAG-tag / His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO2

   Available since

   April 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. P63-sp64 Poly A

   Plasmid

   #59435

   Purpose

   synthetic poly A transcripts of VZV

   Depositor

   Randall Cohrs

   Article

   Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

   Insert

   VZV ORF 63
   

   Use

   Makes synthetic transcripts that contains poly a

   Tags

   none

   Expression

   Mutation

   none

   Promoter

   SP6

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA3-HA/Bmal L95E/L150E/W427A

   Plasmid

   #47352

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E/L150E/W427A

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pGEX-4T3-ZO-2-1st-PDZ-delta-NLS

   Plasmid

   #27425

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of NLS-Nuclear Localization Signal: Nucl…

   Promoter

   Available since

   May 18, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pBABEhygro-Flag-ZO-2-delta-N

   Plasmid

   #27476

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Deletion of coding nucleotides from 1-351. This r…

   Promoter

   Available since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pmiRGLO-TMEM63C-201

   Plasmid

   #117372

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   TMEM63C-2013' UTR
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmiRGLO-TNFRSF10B

   Plasmid

   #117373

   Purpose

   Dual luciferase assay for miRNA-targeted UTR

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   3' UTR TNFRSF10B
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available since

   Dec. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmiRGLO-KIAA2013-201

    Plasmid

    #117348

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR KIAA2013-201
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmiRGLO-FAM102A-203

    Plasmid

    #117344

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR FAM102A-203
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmiRGLO-GRM4-202

    Plasmid

    #117347

    Purpose

    Dual luciferase assay for miRNA-targeted UTR

    Depositor

    Volker Busskamp

    Article

    Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

    Insert

    3' UTR GRM4-202
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Murine PGK-1

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-chCARex

    Plasmid

    #87233

    Purpose

    bacterial expression of GST-CAR extracellular domain

    Depositor

    Fritz Rathjen

    Article

    Patzke et al J Neurosci. 2010 Feb 24;30(8):2897-910.

    Insert

    CXADR (CXADR Chicken)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-HA/Bmal W427A

    Plasmid

    #47349

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    W427A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. P29-sp64 Poly A

    Plasmid

    #59434

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 29
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. HLH Clock Pas 1-401 VenN

    Plasmid

    #47353

    Purpose

    Clock fragment cloned and tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pIDS-RESTmut full-length

    Plasmid

    #109162

    Purpose

    Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length REST/NRSF (REST Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Cys402Ser

    Promoter

    p10

    Available since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmMextli

    Plasmid

    #79262

    Purpose

    Expresses EGFP-tagged DmMextli in S2 cells

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

    Insert

    DmMextli (mxt Fly, NM_164598)
    

    Use

    Schneider 2 cell expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Ac5 promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5 FRT TO myc Separase delta 55/S1126A

    Plasmid

    #59825

    Purpose

    Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

    Insert

    Separase (ESPL1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    delta 55/S1126A

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-HA/Bmal V435R

    Plasmid

    #47350

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    V435R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pBABE-EGFP-THAP11-rescue-3xFLAG

    Plasmid

    #37000

    Depositor

    Debu Chakravarti

    Article

    Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

    Insert

    THAP11 (THAP11 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Mutations were generated at codons 273-275 conver…

    Promoter

    Available since

    May 21, 2012

    Availability

    Academic Institutions and Nonprofits only

22. HLH Clock Pas 1-401 VenN L74E

    Plasmid

    #47355

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L74E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    changed 478 Tyrosine for Alanine

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRN3P-HA-Suv4-20h2mut

    Plasmid

    #86692

    Purpose

    For transcription of of Suv4-20h2mut mRNA preceded by an HA-tag in 5'

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

    Insert

    histone-lysine N-methyltransferase KMT5C mutant (Kmt5c Mouse)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Mutated asparagine 273 to cysteine 276 (NHDC) int…

    Promoter

    T3

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCDNA3-HA/Bmal L150E

    Plasmid

    #47346

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L150E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pFastBac-hDcr-K70A.

    Plasmid

    #89145

    Purpose

    Expression of helicase mutant of human Dicer in Sf9 cells

    Depositor

    Jennifer Doudna

    Article

    MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

    Insert

    hDicer (DICER1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Insect

    Mutation

    K70A

    Promoter

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. P21-sp64 Poly A

    Plasmid

    #59433

    Purpose

    synthetic poly A transcripts of VZV

    Depositor

    Randall Cohrs

    Article

    Cohrs et al J Virol. 2000 Dec;74(24):11464-71.

    Insert

    VZV ORF 21
    

    Use

    Makes synthetic transcripts that contains poly a

    Tags

    none

    Expression

    Mutation

    none

    Promoter

    SP6

    Available since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

28. HLH Bmal Pas 1-465-VenC

    Plasmid

    #47364

    Purpose

    Bmal fragment cloned with Venus tag and used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

29. FHpHG_mTent5c_LxxAA (L3m)

    Plasmid

    #221441

    Purpose

    Transfection into the cells for the reporter or qPCR assay.

    Depositor

    Marcos Morgan

    Article

    Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

    Insert

    Tent5c_LXXAA (L3m) (Fam46c Mouse)
    

    Use

    Tags

    FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

    Expression

    Mammalian

    Mutation

    L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TTR F87E

    Plasmid

    #238075

    Purpose

    Bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F87E

    Promoter

    T7

    Available since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETM11-6xHis-TEV-hORF1p

    Plasmid

    #202589

    Purpose

    Allows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

    Depositor

    Liam Holt, Kathleen Burns

    Article

    Taylor et al Cell. 2013 Nov 21;155(5):1034-48. doi: 1

    Insert

    ORF1p
    

    Use

    Tags

    6x His and TEV protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA4c/His-Xpress-HA-hBIG2(WT)

    Plasmid

    #79432

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA4c/His-Xpress-HA-hBIG1(WT)

    Plasmid

    #79431

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    C1758R

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUC18 3xFLAG COP1

    Plasmid

    #237334

    Purpose

    expression in bacterial cells for protein purification

    Depositor

    Michele Pagano

    Article

    Rizzini et al Curr Biol. 2019 Jun 17;29(12):1954-1962.

    Insert

    COP1 (COP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSMPPv2-Tau-RING (M72E/I18R)

    Plasmid

    #233690

    Purpose

    Expression of Tau-RING I18R-M72E mutant in mammalian cells

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau protein fused to TRIM21 RING domain carrying M72E/I18R mutations
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOPTG-Tau-RING (M72E/I18R)

    Plasmid

    #233692

    Purpose

    Expression of Tau-RING I18R-M72E mutant in bacterial cells

    Depositor

    Leo James

    Article

    Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

    Insert

    Tau protein fused to TRIM21 RING domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRDA355_sgCiPOLR2D

    Plasmid

    #229022

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    POLR2D gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. OPEN-HLA-A*11:01-BirA

    Plasmid

    #235135

    Purpose

    E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

    Depositor

    Nikolaos Sgourakis

    Article

    Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

    Insert

    OPEN-HLA-A*11:01 (HLA-A Human)
    

    Use

    Tags

    BirA

    Expression

    Bacterial

    Mutation

    G120C

    Promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRDA355_sgCiPELO #2

    Plasmid

    #229020

    Purpose

    Expression of a CRISPRi doxycycline inducible guide targeting PELO

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    PELO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRDA052H_sgCh2

    Plasmid

    #229023

    Purpose

    Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRDA_174_neoR

    Plasmid

    #228935

    Purpose

    Lentiviral expression of EnAsCas12a

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRDA052H_sgFOCAD #2

    Plasmid

    #229024

    Purpose

    Expression of a EnAsCas12a guide targeting FOCAD

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    FOCAD gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pXPR_051d_sgCiChr2-2

    Plasmid

    #228940

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    Francisca Vazquez

    Article

    Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

    Insert

    sgChr2-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pTMiniTrex-mCherry-Shark3

    Plasmid

    #233145

    Purpose

    Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark3
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Expression

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA3.1-myc:FRB:gs10:Cul1(dC501)

    Plasmid

    #228533

    Purpose

    A fragment of humanCul1 protein in fusion with FRB domain through a flexible glycine-serine linker.

    Depositor

    Roman Jerala

    Article

    Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

    Insert

    myc:FRB:gs10:Cul1(dC501) (CUL1 Synthetic, Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. VPS13B^Halo

    Plasmid

    #224584

    Purpose

    codon optimized Halo tagged VPS13B for expression in mammalian cells

    Depositor

    Pietro De Camilli

    Article

    Ugur et al bioRxiv [Preprint]. 2023 Dec 18:2023.12.

    Insert

    VPS13B^Halo (VPS13B Human)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCA528 IST1 (1-366)

    Plasmid

    #193039

    Purpose

    Bacterial expression plasmid for full-length IST1 (1-366).

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pCA528 CAPN7 (1-75)

    Plasmid

    #193031

    Purpose

    Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 1-75

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCA528 CAPN7 (81-165)

    Plasmid

    #193032

    Purpose

    Bacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domain

    Depositor

    Wesley Sundquist

    Article

    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

    Insert

    CAPN7 (CAPN7 Human)
    

    Use

    Tags

    6xHis-Sumo

    Expression

    Bacterial

    Mutation

    Residues 81-165

    Promoter

    T7

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid

    #227333

    Purpose

    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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