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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,547 results for: CHL

Showing: 7101 - 7150 of 10547 results

  1. pGL3-LRR

    Plasmid
    #66937
    Purpose
    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Reverse orientation (attR2, attR1).
    Depositor
    Alvaro Monteiro
    Article
    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV-40
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pCVD438

    Plasmid
    #63759
    Purpose
    eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pGWB401NL3F10H

    Plasmid
    #141285
    Purpose
    No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Type
    Empty backbone
    Use
    Luciferase; Gateway
    Tags
    FLAG (x3), HIS (x10), and NLUC
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Feb. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    141285-plasmid-map-sequence-id-324626

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  26. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

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  37. cacna2d1 R241A D491A HA pcDNA3

    Plasmid
    #206109
    Purpose
    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding
    Depositor
    Annette Dolphin
    Insert
    cacna2d1 (Cacna2d1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    R241A D491A
    Promoter
    CMV
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    206109-plasmid-map-sequence-id-415382

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  46. pTU2-a-RFP

    Plasmid
    #72958
    Purpose
    Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 2 - a site
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  47. pKS011

    Plasmid
    #65464
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
    Depositor
    Keith Tyo
    Article
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    MBP-Ntag-mCherry
    Use
    Tags
    FLAG and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    65464-plasmid-map-sequence-id-116873

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Showing: 7101 - 7150 of 10547 results