We narrowed to 7,692 results for: Lif;
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Plasmid#175207PurposeXenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYCR075C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Cdc42-T17N
Plasmid#214289PurposeExpress dominant negative Cdc42 fused with MBPDepositorInsertDominant negative Cdc42 (CDC42 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationCdc42-T17NPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-Q61L
Plasmid#214292PurposeExpress constitutively active Rac1 fused with MBPDepositorInsertConstitutively active Rac1 (RAC1 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-Rac1-T17N
Plasmid#214293PurposeExpress dominant negative Rac1 fused with MBPDepositorInsertDominant negative Rac1 (RAC1 Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-T17NPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-RhoA-Q63L
Plasmid#214296PurposeExpress constitutively active RhoA fused with MBPDepositorInsertConstitutively active RhoA (RHOA Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-Q63LPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-MBP-RhoA-T19N
Plasmid#214297PurposeExpress dominant negative RhoA fused with MBPDepositorInsertDominant negative RhoA (RHOA Human)
TagsMaltose-binding protein (MBP)ExpressionBacterialMutationRhoA-T19NPromoterTacAvailable SinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GTS-TurboID-GFP
Plasmid#209405PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Golgi targeting sequence (GTS) in plantaDepositorInsertGTS-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-mTP-TurboID-GFP
Plasmid#209404PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a Mitochondrial transit peptide (mTP) in plantaDepositorInsertmTP-3XHA-TurboID
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD
Plasmid#199447PurposeExpession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signalDepositorInsertdSaCas9-10xGCN4
UseLentiviralExpressionMammalianPromoterhuman ubiquitin C (UBC)Available SinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-dsRNAres_I
Plasmid#146577PurposeInsect Expression of DmeIF4GaDepositorInsertDmeIF4Ga (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENmRFPCNAL2 pc1054
Plasmid#167567PurposeThe fusion protein contains a SV40 NLS followed by mRFP, linker and human PCNA.DepositorAvailable SinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
hPCNA-S228I
Plasmid#190938Purposeuntagged human PCNA with a hypomorphic disease-associated serine to isoleucine mutation at residue 228DepositorInsertProliferating Cell Nuclear Antigen (PCNA Human)
ExpressionBacterialMutationchanged serine 228 to isoleucinePromoterT7Available SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0065
Plasmid#177019PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase
UseSynthetic BiologyMutationtransit peptide removed, amplified from cDNA, con…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-AARS-32
Plasmid#166540PurposescFv of a human scaffold targeting Alanyl-tRNA synthetase. Antigen coverage aa 1-968 of 968, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ad-MKP-3 153-381
Plasmid#131524PurposeExpresses MKP-3 153-381 in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoMutationonly reserved amino acid 153-381PromoterCMVAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-5X-1-MePCE_aa470-689
Plasmid#113544PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa432-689
Plasmid#113543PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa400-689
Plasmid#113542PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-1_MePCE_aa1-400
Plasmid#113541PurposeProduces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.DepositorAvailable SinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL5B
Plasmid#67463PurposeMammalian expression of hArl5b with stop codon (native expression)DepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARF5-V5-His6
Plasmid#67414PurposeExpression of hArf5 with C-term V5 and His6 tag in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL6
Plasmid#67380PurposeExpression of native hArl6 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL4C
Plasmid#67381PurposeExpression of native hArl4c in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL8A
Plasmid#67382PurposeExpression of native hArl8 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL14
Plasmid#67385PurposeExpression of native hArl14 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL11
Plasmid#67386PurposeExpression of native hArl11 in E. coliDepositorInsertARL11 (ARL11 Human)
ExpressionBacterialMutationbp 261 G to A; silent mutation; bp 442 T to C; aa…PromoterT7Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARFRP1
Plasmid#67387PurposeExpression of native hArfrp in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SARA
Plasmid#67389PurposeExpression of native hSara in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARF1
Plasmid#67369PurposeExpression of native hArf1 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARF3
Plasmid#67370PurposeExpression of native hArf3 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARF5
Plasmid#67372PurposeExpression of native hArf5 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARF6
Plasmid#67373PurposeExpression of native hArf6 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL1
Plasmid#67374PurposeExpression of native hArl1 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL2
Plasmid#67375PurposeExpression of native hArl2 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL4
Plasmid#67377PurposeExpression of native hArl4 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL5A
Plasmid#67378PurposeExpression of native hArl5 in E. coliDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5B
Plasmid#67326PurposehArl5b with stop codon in Gateway Entry vectorDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-R5A-K6A C11orf83-V5
Plasmid#65850PurposeMammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5A-K6ADepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-R5D-K6D C11orf83-V5
Plasmid#65851PurposeMammalian expression of C terminally V5-tagged C11orf83/UQCC3 : MUTANT R5D-K6DDepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR
Plasmid#65091PurposeDestination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) for stable cell line generationDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged sequence to render plasmid resistant to s…PromoterCMV, 2x TetAvailable SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_111del
Plasmid#65659PurposeFLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationintroduced stop codon such as to produce deletion…PromoterCMV, 2x TetAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_E97A_K100A
Plasmid#65660PurposeFLAG-HA expression vector with RBPMS carrying mutation E97A, K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged glutamic acid 97 to alanine, lysine 100 t…PromoterCMV, 2x TetAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR™221_RBPMS2
Plasmid#59389PurposepDONR vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
PromoterT7Available SinceFeb. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
DIRAS2
Plasmid#55670Purposeexpression of mouse DIRAS2DepositorAvailable SinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCru5-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49222PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterViral LTR (or CMV)Available SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49231PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-GGG/ACC-mEGFP-IRES-mCherry
Plasmid#49230PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only