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Plasmid#236581PurposeISYmu1 CHLl1 gRNA7 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW2156
Plasmid#236580PurposeISYmu1 CHLl1 gRNA6 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW2066
Plasmid#236575PurposeISYmu1 PDS3 gRNA2 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW2153
Plasmid#236577PurposeISYmu1 CHLl1 gRNA3 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW2159
Plasmid#236582PurposeISYmu1 CHLl1 gRNA9 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTW2155
Plasmid#236579PurposeISYmu1 CHLl1 gRNA5 TRV2 Architecture_BDepositorInsertISYmu1 TnpB
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHOSPHO2-TEV-Twin-Strep
Plasmid#222016PurposeExpress human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorInsertPHOSPHO2 (PHOSPHO2 Human)
UseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationdeleted stop codon (TAA)Promoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKanB
Plasmid#223523PurposeUsed for expression of intracellular recombinant protein in Pichia pastoris; with G418 resistance gene as selectable markerDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAOX1Available sinceSept. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Tol2-U6.3-sgRNA-non-targeting-control -GFP
Plasmid#221844PurposeTol2 transposon expresses control non-targeting sgRNA from chick U6.3 promoter expresses GFP reporter from GAGC promoterDepositorInsertsEGFP
non-targeting control sgRNA- GCACTGCTACGATCTACACC
UseCRISPR; Tol2 transposon optimised for chick expre…TagsExpressionMammalianMutationPromoterGACG and U6.3 chickAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSK519-cspB
Plasmid#207162PurposeExpresses codon-optimized cspB from Clavibacter michiganensis in a broad host range vectorDepositorInsertcspB
UseTagsHAExpressionBacterialMutationPromoternptIIAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB602-RBCS-tagRFP
Plasmid#213868PurposeBinary vector for the expression of RBCS fused to the tagRFPn plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertRIBULOSE BISPHOSPHATE CARBOXYLASE SMALL CHAIN 1A (RBCS) (RBCS1A Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY380
Plasmid#182962PurposeAmp-resistant, low copy (p15A ori), E. coli 5'ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDY390
Plasmid#182963PurposeAmp-resistant, low copy (p15A ori), E. coli ptsI transcriptionally fused with GFP.DepositorInsertptsI
UseSynthetic BiologyTagsGFPExpressionMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-VC155
Plasmid#194051PurposeTransient expression of VN155 (mVenus β-strands 8 to 11, amino acids 155−239) in plant cell (Cytosol)DepositorInsertVC155 (mVenus β-strands 8 to 11, amino acids 155−239)
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-AtOEP7(1-50aa)-mVenus
Plasmid#194000PurposeTransient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)DepositorInsertmVenus
UseTagsAtOEP7 1–50aaExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWT35S-VN154
Plasmid#194050PurposeTransient expression of VN154 (mVenus β-strands 1 to 7, amino acids 1−154) in plant cell (Cytosol)DepositorInsertVN154 (mVenus β-strands 1 to 7, amino acids 1−154)
UseTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRnc-gGFP129
Plasmid#189537PurposeExpresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-129-GFPDepositorInsertsRNase III
gUTR-129-GFP
UseTagsExpressionBacterialMutationN/APromoterJ23119 and pBADAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BLZinCh-P40
Plasmid#188057PurposeExpression of BLZinCh-P40, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cellsDepositorInsertBLZinCh-P40
UseTagsExpressionMammalianMutationPromoterCMV promotorAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BLZinCh-P50
Plasmid#188058PurposeExpression of BLZinCh-P50, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cellsDepositorInsertBLZinCh-P50
UseTagsExpressionMammalianMutationPromoterCMV promotorAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pW299-lenti-spsgRNA-Esp3I-2kb-filler-pEF1s-NLS-tagBFP-P2A-BlastR
Plasmid#189943PurposeLentiviral vector to co-express an spsgRNA with NLS-tagBFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP7_rpoA-U
Plasmid#190974PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP7
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-E67A_rpoA-U
Plasmid#190959PurposeTest the U-to-C editing activity of KP2:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP6-A66S_rpoA-C
Plasmid#190968PurposeTest the C-to-U editing activity of KP6:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP6
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP5_rpoA-U
Plasmid#190985PurposeTest the U-to-C editing activity of KP5 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP5
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP1_rpoA-U
Plasmid#190955PurposeTest the U-to-C editing activity of KP1 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP1
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP2-A66S_rpoA-U
Plasmid#190957PurposeTest the U-to-C editing activity of KP2:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP2
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-A66S_rpoA-U
Plasmid#190961PurposeTest the U-to-C editing activity of KP3:A66S on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
UseTagsHisExpressionBacterialMutationDYW-KP:A66SPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PM18033-PLS-KP7_rpoA-U
Plasmid#190994PurposeTest the U-to-C editing activity of KP7 on AtrpoA editing site, in human cellsDepositorInsertPLS-KP7
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP3-E67A_rpoA-U
Plasmid#190963PurposeTest the U-to-C editing activity of KP3:E67A on AtrpoA editing site, in E. coliDepositorInsertPLS-KP3
UseTagsHisExpressionBacterialMutationDYW-KP:E67APromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PLS-KP4_rpoA-U
Plasmid#190964PurposeTest the U-to-C editing activity of KP4 on AtrpoA editing site, in E. coliDepositorInsertPLS-KP4
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only