We narrowed to 29,737 results for: REP

1. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

   Plasmid

   #197885

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMy-CTCF-GFP

   Plasmid

   #50574

   Purpose

   Retroviral vector endoding for mouse CTCF fused to GFP

   Depositor

   Rafael Casellas

   Article

   Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

   Insert

   Ampicilin
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRY2clust-mCherry-RANK

   Plasmid

   #208612

   Purpose

   Mammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)

   Depositor

   Tomohiro Ishii, Takao Nakata

   Article

   Takada et al Sci Rep. 2024 Jan 19;14(1):1749. doi: 10

   Insert

   CRY2clust-mCherry-RANK (Cry2 Mustard Weed, Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KS-attB2-SA(1)-T2A-Gal4DBD-Hsp70

   Plasmid

   #62903

   Purpose

   Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4DBD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTet-O-NEUROG1-T2A-PuroR

   Plasmid

   #162339

   Purpose

   Lentiviral expression of NEUROG1 under the control of the TetON promoter

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   NEUROG1 (NEUROG1 Human)
   

   Use

   Lentiviral

   Tags

   T2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   Tet-ON

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-SaCas9-U6-sgGria2

   Plasmid

   #124854

   Purpose

   Mutagenesis of Gria2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria2 (Gria2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70

   Plasmid

   #62915

   Purpose

   Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-p65AD-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

   Plasmid

   #62898

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST40-2XFL-Src-S32-KD

    Plasmid

    #140311

    Purpose

    Expresses Flag-tagged human Src-S32-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    Contains only the SH3, SH2, and KD regions

    Promoter

    CMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST40-2XFL-Src-S4-KD

    Plasmid

    #140297

    Purpose

    Expresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studies

    Depositor

    Mark Moasser

    Article

    Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

    Insert

    Src deletion mutant (SRC Human)
    

    Use

    Tags

    2X Flag

    Expression

    Mammalian

    Mutation

    contains only the SH4 and KD regions

    Promoter

    CMV

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSFDuet-1_VNp15-StefinA

    Plasmid

    #182405

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-stefin (CSTA Human)
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-KS-attB2-SA(0)-T2A-Gal4DBD-Hsp70

    Plasmid

    #62902

    Purpose

    Creating Gal4DBD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-Gal4DBD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-STIM1-Venus-173-C

    Plasmid

    #87619

    Purpose

    Full-length STIM1 fused to C-terminal Venus (YFP) fragment; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    STIM1-Venus173-C (STIM1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

    Plasmid

    #115789

    Purpose

    Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

    Depositor

    Harald Junge

    Article

    Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

    Insert

    NDP (NDP Human)
    

    Use

    Tags

    alkaline phosphatase and flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. ACTIN-SOX2-PGK Cre

    Plasmid

    #59019

    Purpose

    Bicistronic Lentivirus expressing sox2 and Cre

    Depositor

    Trudy Oliver

    Article

    Mukhopadhyay et al Cell Rep. 2014 Jul 10;8(1):40-49. doi: 1

    Insert

    Sox2 (Sox2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    B-actin

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

    Plasmid

    #197893

    Purpose

    Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-FLAG-Sestrin3

    Plasmid

    #72592

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN3 (SESN3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

    Plasmid

    #62948

    Purpose

    Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-LexA::QFAD-Hsp70
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p.UTA.2.0 miR-27a-3p

    Plasmid

    #82438

    Purpose

    Dual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p

    Depositor

    Jens Gruber

    Article

    Lemus-Diaz et al Sci Rep. 2017 Mar 24;7:45197. doi: 10.10

    Insert

    hsa-miR-27a-3p (MIR27A Human)
    

    Use

    Tags

    Expression

    Mutation

    Three copies hsa-miR-27a-3p Complementary region …

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pNH-TrxT-G-S18

    Plasmid

    #127827

    Purpose

    Production of recombinant human ribosomal protein S18

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPS18 (RPS18 Human)
    

    Use

    Tags

    His6 - Thioredoxin - TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid

    #154775

    Purpose

    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAA in GFP reporter, UUA anticodon and G10A, …

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLenti GW V5 Eco/Dam mse EMD

    Plasmid

    #182675

    Purpose

    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

    Depositor

    Bas van Steensel

    Article

    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    E. Coli Dam and V5

    Expression

    Mammalian

    Mutation

    Promoter

    HSP

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-Lyso-AktAR2

    Plasmid

    #64933

    Purpose

    Lysosome-targeted FRET biosensor for monitoring Akt activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-AktAR2 (LAMP1 Budding Yeast, Synthetic, Human)
    

    Use

    Tags

    Cerulean3, Full-length LAMP1, and cpVenus[E172]

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

    Plasmid

    #162337

    Purpose

    Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6; mU6

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR-mCherry-attP(C31)-*BleoR

    Plasmid

    #183752

    Purpose

    phiC31 landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    FRT-mCherry-attP-BleoR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge