163,606 results

1. pAAV-hSyn-GRAB-rDA3h

   Plasmid

   #208703

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h in neurons

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA3h
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. p23-puro-EGFP-RPA194

   Plasmid

   #179237

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   RPA194 (POLR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1alpha

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lamp1-RFP

   Plasmid

   #1817

   Purpose

   Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Use

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Promoter

   Available Since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

   Plasmid

   #197687

   Purpose

   plasmid for expressing the DNMT3bCD

   Depositor

   Michael Elowitz

   Article

   Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

   Insert

   H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Synthetic, Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFlp-Km

   Plasmid

   #222334

   Purpose

   Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cl, flp, and sacB

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1 Lifeact-EGFP

   Plasmid

   #58470

   Purpose

   Expresses a cytoplasmic actin filament reporter, the peptide Lifeact, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Bioarchitecture. 2015 Aug 28:1-14.

   Insert

   Lifeact
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tet-O-FUW-ZEB2-P2A-mCherry

   Plasmid

   #203857

   Purpose

   Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   ZEB2 (ZEB2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLSB-KAN

   Plasmid

   #166700

   Purpose

   Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.

   Depositor

   Robin Allshire

   Article

   Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do

   Inserts

   tRNA promoter : sgRNA cassette
   adh15 promoter : Cas9 codon-optimised for S. pombe
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   adh15 promoter and tRNA Ser promoter

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Ef1a-CreN-InteinN

   Plasmid

   #187614

   Purpose

   AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

   Depositor

   Il Hwan Kim

   Article

   Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

   Insert

   CreN-InteinN
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSIQ

    Plasmid

    #216238

    Purpose

    All-in One IQ transgene switch, empty vector

    Depositor

    Hyunju Ro

    Article

    Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

    Plasmid

    #148828

    Purpose

    Mammalian Expression of HsNot9-shRNA2res

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9-shRNA2res (CNOT9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB-GST-3C-CDK1

    Plasmid

    #177011

    Purpose

    Generate baculovirus for insect cell expression of CDK1 with N-terminal GST-3C (PreScission)

    Depositor

    Andrea Musacchio

    Article

    Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

    Insert

    CDK1 (CDK1 Human)
    

    Use

    Tags

    GST-3C

    Expression

    Insect

    Mutation

    codon-optimised for insect cell expression

    Promoter

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE7.10-F148A

    Plasmid

    #132946

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    ABE7.10(F148A)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    ABE7.10(F148A)

    Promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK003

    Plasmid

    #236570

    Purpose

    Cloning vector for PaqCI Golden Gate assembly of ISDra2 guides into ISDra2 plant expression vector

    Depositor

    Steven Jacobsen

    Article

    Weiss et al Nat Plants. 2025 Apr 22. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas-CmR(+)

    Plasmid

    #113633

    Purpose

    Chloramphenicol resistant derivative of pCas plasmid

    Depositor

    Olivier Lichtarge

    Article

    Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

    Insert

    Chloramphenicol resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Pcat

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-ROCK1

    Plasmid

    #187296

    Purpose

    Express GFP ROCK1

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock1 (a.k.a. 1110055K06Rik, Rock-I) (Rock1 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)Flag-His-ATM wt

    Plasmid

    #31985

    Purpose

    Mammalian expression of human ATM with Flag and His tags

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM wild-type (ATM Human)
    

    Use

    Tags

    Flag and HIS

    Expression

    Mammalian

    Mutation

    3' UTR of 400 nucl.

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF1329

    Plasmid

    #143789

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2A (TFAP2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pPB-cT3G-cERP2-FOXL2

    Plasmid

    #192914

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 (FOXL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. TFORF0144

    Plasmid

    #141536

    Purpose

    Lentiviral vector for overexpressing the PHOX2A transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PHOX2A (PHOX2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTUM4

    Plasmid

    #230905

    Purpose

    Plasmid encoding four periplasmic folding helper proteins to boost the secretion of functional heterologous proteins in E. coli

    Depositor

    Arne Skerra

    Article

    Schlapschy et al Protein Eng Des Sel. 2006 Aug;19(8):385-

    Inserts

    FkpA peptidyl-prolyl cis-trans isomerase FkpA (fkpA E. coli)
    SurA (surA E. coli)
    DsbA (dsbA E. coli)
    DsbC (dsbC E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pExp-sfGFP

    Plasmid

    #112573

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis, Strep II, and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-TAC-ERP2-(N-HA)KRAS_G12D

    Plasmid

    #201254

    Purpose

    Dox-inducible overexpression of HA-tagged KRASG12D and mCherry in human cells

    Depositor

    Alexander Kleger

    Article

    Breunig et al Cell Stem Cell. 2021 Jun 3;28(6):1105-11

    Insert

    KRAS (KRAS Human)
    

    Use

    Piggybac transposon

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    changed Gly (G) 12 to Asp (D)

    Promoter

    TRE

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mPapaya-pBAD

    Plasmid

    #54838

    Purpose

    Localization: Protein Expression Vector , Excitation: 530, Emission: 541

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mPapaya

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

    Plasmid

    #232879

    Purpose

    Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    5-Hydroxytryptamine Receptor 6 (HTR6 Human)
    

    Use

    Lentiviral

    Tags

    RGECO1.1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mc2 puro

    Plasmid

    #206215

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac-His-MBP-SCov2-nsp12

    Plasmid

    #154759

    Purpose

    Expression of SARS-CoV-2 nsp12

    Depositor

    Patrick Cramer

    Article

    Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge