159,284 results

1. Lenti-Cre

   Plasmid

   #198712

   Purpose

   For lentiviral expression of Cre recombinase in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Insert

   Pgk-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. HaloTag-sec61b

   Plasmid

   #186959

   Purpose

   Express sec61b connected with N-terminus HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   Sec61 (SEC61B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1a-mCherry-EGFP-LC3B

   Plasmid

   #170446

   Purpose

   Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

   Depositor

   Sang-Hun Lee

   Article

   Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

   Insert

   mCherry-GFP-LC3 (MAP1LC3B Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAP05

   Plasmid

   #186261

   Purpose

   sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter

   Depositor

   Yongku Cho

   Article

   Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

   Inserts

   sfGFP
   EL222 (ELI_04755 Erythrobacter litoralis)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-IRES-GFP

   Plasmid

   #20672

   Purpose

   Depositor

   Tannishtha Reya

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1_blasti

   Plasmid

   #194067

   Purpose

   3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blasti

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCL20C-ddIPpoI

   Plasmid

   #49053

   Purpose

   Expresses the restriction enzyme IPpoI under the control of Shield1

   Depositor

   Michael Kastan

   Article

   Goldstein et al Proc Natl Acad Sci U S A. 2013 Sep 30.

   Insert

   DD-HA-ER-IPpoI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-TEV protease

   Plasmid

   #165509

   Purpose

   Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   TEV protease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. EGFP-Tubulin-6

   Plasmid

   #56450

   Purpose

   Localization: Microtubules, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Cry2olig

    Plasmid

    #208780

    Purpose

    Inducibly expresses light activatable Cry2 domain

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Cry2 mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-mCherry

    Plasmid

    #41583

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. TagBFP2-CAAX

    Plasmid

    #116856

    Purpose

    plasma membrane marker

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    HRAS
    

    Use

    Tags

    TagBFP2

    Expression

    Mammalian

    Mutation

    Amino acids 172-189

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX-Tet3G blasticidin

    Plasmid

    #128061

    Purpose

    Lentiviral vector encoding the Tet-On 3G transactivator protein, which allows for inducible gene expression. With Blasticidin resistance.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS2191_Nme2-evoFERNY-nt

    Plasmid

    #201527

    Purpose

    Mammalian expression of N-terminally fused Nme2-evoFERNY-nt

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-evoFERNY-nt
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. tetO-SMAD6

    Plasmid

    #170700

    Purpose

    doxycycline-inducible overexpression of SMAD6

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SMAD6 (SMAD6 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS578_DD-dSpyCas9-mCherry-APEX2

    Plasmid

    #108570

    Purpose

    Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Insert

    dSpyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    APEX2, Destabilization domains, Flag, and mCherry…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-Kif5c

    Plasmid

    #71853

    Purpose

    mammalian expression of GFP-Kif5c

    Depositor

    Michelle Peckham

    Article

    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

    Insert

    Kif5c (Kif5c Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kif5c starts at AA3

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pHN781

    Plasmid

    #178828

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Use

    Tags

    His8, TEV

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pSEVA351-Cpf1

    Plasmid

    #192361

    Purpose

    CRISPR-based vector for genome editing

    Depositor

    Juana María Navarro Llorens

    Article

    Baldanta et al Microb Cell Fact. 2022 May 28;21(1):103.

    Insert

    Cas12a, gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pC018 - LshCas13a from Leptotrichia shahii

    Plasmid

    #91908

    Purpose

    Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LshCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TFORF2538

    Plasmid

    #144036

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX4 (SOX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. STIM1-EGFP

    Plasmid

    #210370

    Purpose

    Expresses STIM1 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tetO-MYOCD

    Plasmid

    #170696

    Purpose

    doxycycline-inducible overexpression of MYOCD

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    MYOCD (MYOCD Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET51b-His-TEV-HaloTag7

    Plasmid

    #167266

    Purpose

    Overexpression of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mouse b3 full length

    Plasmid

    #217814

    Purpose

    Full length mouse integrin b3 expression

    Depositor

    Timothy Springer

    Article

    Yuxin et al bioRxiv 2024.01.26.577394

    Insert

    ITGB3 (Itgb3 Mouse)
    

    Use

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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