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We narrowed to 19,107 results for: Tec

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Showing: 7251 - 7300 of 19107 results

  4. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

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  12. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid
    #112031
    Purpose
    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)
    Depositor
    Jesse Rinehart
    Article
    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.
    Insert
    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    Tags
    Split mCherry
    Expression
    Bacterial
    Available Since
    July 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112031-plasmid-map-sequence-id-216275

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  14. pEDF-PhdRS

    Plasmid
    #127445
    Purpose
    Double mutant of wild type mmPylRS designed for incorporation of non-cannonical amino acids in to M13 bacteriophage
    Depositor
    Wenshe Liu
    Article
    Tharp et al Nat Commun. 2020 Mar 13;11(1):1392. doi:
    Insert
    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    Expression
    Bacterial
    Mutation
    N346A-C348A mutations on PylRS
    Promoter
    araBAD
    Available Since
    April 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    127445-plasmid-map-sequence-id-250213

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  17. pOCC119

    Plasmid
    #118884
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease and mGFP, cl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118884-plasmid-map-sequence-id-232361

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  18. pYPQ266

    Plasmid
    #164713
    Purpose
    Gateway entry clone (attL1 & attR5) PmCDA1-CBE_V01 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    zCas9(D10A)-PmCDA1-UGI
    Use
    CRISPR; Gateway compatible pmcda1-cbe_v01 entry c…
    Expression
    Plant
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    164713-plasmid-map-sequence-id-325387

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  19. MSP2253

    Plasmid
    #70704
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70704-plasmid-map-sequence-id-127764

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  24. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid
    #128402
    Purpose
    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Tetanus toxin light chain domain
    Tags
    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…
    Expression
    Bacterial
    Mutation
    Inactivating E234A mutation
    Promoter
    trc
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128402-plasmid-map-sequence-id-263357

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  33. pREMI-ZA

    Plasmid
    #183736
    Purpose
    Plasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffii
    Depositor
    Jun Ishii
    Article
    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1
    Insert
    Sh ble
    Use
    Random insertional gene deletion in komagataella …
    Promoter
    TEF1 promoter
    Available Since
    March 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    183736-plasmid-map-sequence-id-372719

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  34. pCosSAD1AprR

    Plasmid
    #190786
    Purpose
    An E. coli cloning vector for the integration of heterologous genes into a linear plasmid pSA3239 in Streptomyces lavendulae subsp. lavendulae CCM 3239 through homologous recombination
    Depositor
    Jan Kormanec
    Article
    Novakova et al Appl Microbiol Biotechnol. 2022 Nov;106(
    Inserts
    upstream region of the aur1 cluster for auricin
    ampramycin resistance cassette
    downstream region of the aur1 cluster for auricin
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    190786-plasmid-map-sequence-id-375582

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  35. pIMVAd39T

    Plasmid
    #98303
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or GAL2 promoter; integrated into PDC5 locus; disrupting PDC5.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    pdc5(-277,-32)-PGAL2>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1
    Expression
    Yeast
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98303-plasmid-map-sequence-id-182779

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  47. evoCas9

    Plasmid
    #138558
    Purpose
    Expresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    evoCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    M495V, Y515N, K526E, R661Q
    Promoter
    EFS
    Available Since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    138558-plasmid-map-sequence-id-274774

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  50. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

    Plasmid
    #181787
    Purpose
    Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181787-plasmid-map-sequence-id-354706

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Showing: 7251 - 7300 of 19107 results