We narrowed to 18,361 results for: met

1. pEB2-DsRed-Express2

   Plasmid

   #103999

   Purpose

   Plasmid encoding DsRed-Express2

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   DsRed-Express2
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to DsRed (R2D, S4T, K5E, E10P,…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mEmerald-MAP4-C-10

   Plasmid

   #54152

   Purpose

   Localization: Microtubules, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

   Insert

   MAP4
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   aa 640-1045 of MAP4

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pCFJ909

   Plasmid

   #44480

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, unc-119, MCS
   

   Use

   Multiple cloning site vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SMVP-Cas9N-U6-gRNA P1

   Plasmid

   #80943

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Puro-CAG-VSFP-CR

   Plasmid

   #40257

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   VSFP-CR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTE105

   Plasmid

   #59400

   Purpose

   MethyloBrick expression vector, tuf promoter; TcR

   Depositor

   Tobias Erb

   Article

   Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptuf

   Available Since

   Dec. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFlag-Dam

   Plasmid

   #121909

   Purpose

   Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Dam human codon optimized (dam E. coli)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH183_MS2-AIDΔDead-Hygro

   Plasmid

   #85414

   Purpose

   lentiviral expression vector for MS2-AIDΔDead fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AIDΔDead and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. mEos4b-Lifeact-7

   Plasmid

   #54813

   Purpose

   Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Use

   Tags

   mEos4b-Lifeact

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pDS220

    Plasmid

    #34980

    Purpose

    Expresses a low-affinity ePDZ variant fused to mCherry in yeast. Illumination with 440 nm light induces binding to LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    ePDZb–mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLI-2xFKBP-GFP(1463000)

    Plasmid

    #85789

    Purpose

    P. falciparum integration plasmid to fuse endogenous genes with tags using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
    

    Use

    P. falciparum integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. M-ST1n-VP64

    Plasmid

    #48675

    Purpose

    Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9-VP64, nuclease-null
    

    Use

    CRISPR

    Tags

    NLS and VP64

    Expression

    Mutation

    nuclease-null (D9A D598A H599A N622A)

    Promoter

    CMV

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-FLEX-CAG-CyRFP1

    Plasmid

    #84357

    Purpose

    Expresses CyRFP1 fluorescent protein in an AAV backbone, FLEX version (Cre dependent)

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    CyRFP1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Double FLEXed

    Promoter

    CAG

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CT3G-CERP2

    Plasmid

    #175499

    Purpose

    All-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct:dCas9

    Plasmid

    #78903

    Purpose

    Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSETA-qmTQ2-citrate

    Plasmid

    #225251

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-citrate
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLI-N-sandwich-loxP(K13)

    Plasmid

    #85793

    Purpose

    P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    Kelch13
    

    Use

    Cre/Lox; P. falciparum genomic integration

    Tags

    double 2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-smURFP-T2A-mCherry

    Plasmid

    #80347

    Purpose

    Lentiviral transfer vector under the CMV promoter that expresses smURFP and mCherry.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    smURFP
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEB2-TagRFP-T

    Plasmid

    #103998

    Purpose

    Plasmid encoding TagRFP-T

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP-T
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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