We narrowed to 7,778 results for: CCH
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Plasmid#17654DepositorTagsmRFPExpressionMammalianMutationC655G mutation in the glucocorticoid receptor (se…Available SinceMarch 28, 2008AvailabilityAcademic Institutions and Nonprofits only
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pCast
Plasmid#200169PurposeE. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozymeDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FLAG-APEX2-NES-NatMX6
Plasmid#229231PurposeTemplate plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeastDepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
TagsHA tag and myc tagExpressionYeastPromoterGAL1Available SinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYSD099
Plasmid#212923PurposeA MoClo Yeast Toolkit LEU2, CEN/ARS empty vector (GFP dropout). Contains ConL1, part234 GFP dropout, ConR2, LEU2, CEN6/ARS4, AmpR-ColE1.DepositorInsertGFP
ExpressionBacterialAvailable SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188-PrARG8-GFP::URA3
Plasmid#216512PurposeIntegrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrARG8-yeGFP
UseCre/Lox; IntegrativeExpressionYeastPromoterARG8Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfB2197-PrGNP1-mK2-NatMX
Plasmid#216513PurposeIntegrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeastDepositorInsertPrGnp1-mKate2
UseCre/Lox; IntegrativeExpressionYeastPromoterGNP1Available SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Plasmid EUPp_09
Plasmid#157745PurposePgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)DepositorInsertsExpressionBacterialPromoterPgyrAAvailable SinceJune 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Bs-1
Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianPromotergfaABC1DAvailable SinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEK36
Plasmid#210598PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
TagsmClover3ExpressionBacterial and YeastPromoterSpH2BproAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE-SHH
Plasmid#134350PurposeTargeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locusDepositorInsertSHH (SHH Human)
ExpressionMammalianAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLOX-TERT-iresTK
Plasmid#12245Purpose2nd generation lentiviral vector for reversible immortalization of human primary cellsDepositorInsertTERT, HSV1-TK (TERT Human)
UseCre/Lox and LentiviralExpressionMammalianMutationLoxP in 3' LTR.Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMK419 (GAL-OsTIR1(F74G))
Plasmid#140656PurposeGAL-OsTIR1(F74G)DepositorInsertGAL-OsTIR1(F74G)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
HDAC5 Flag
Plasmid#13822PurposeMammalian expression of human histone deacetylase 5 with flag tagDepositorAvailable SinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pK036.TRE-Flpe-WPRE (Supernova)
Plasmid#85007PurposeFor Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.DepositorInsertNLS-FLPe-WPRE
ExpressionMammalianAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP703
Plasmid#136567PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertH2B-emiRFP703
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH2B-emiRFP670
Plasmid#136571PurposeHistone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertH2B-emiRFP670
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-8
Plasmid#163136PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterP(rhaB)Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP703-tubulin
Plasmid#136564PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)DepositorInsertemiRFP703-tubulin
TagsemiRFP703ExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p403-BrdU-Inc
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTA-Mob 2.0
Plasmid#149662PurposeConjugation helper plasmid for use in cis (self-transfer) and trans (transfer of plasmids with RK2/RP4 origin of transfer) mobilization to prokaryotic and eukaryotic cells.DepositorTypeEmpty backboneUseSynthetic Biology; Conjugation helper plasmidExpressionBacterialAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLOX-CWBmi1
Plasmid#12240DepositorInsertBmi1 (Bmi1 Mouse)
UseCre/Lox and LentiviralExpressionMammalianMutationCMV promoter upstream of mBmi-1. LoxP in 3' …Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCIG3 (pCMV-IRES-GFP version 3)
Plasmid#78264PurposeLentiviral empty cloning vector containing IRES and GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsIRES-EGFPExpressionMammalianPromoterCMV, lentiviral LTRAvailable SinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
Plasmid#51690PurposeYeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisGDepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-emiRFP670
Plasmid#136570PurposeLAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertLAMP1-emiRFP670
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP
Plasmid#86827PurposeExpresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAsDepositorInsertFLAG-NLS-MS2-eGFP
ExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pemiRFP670-tubulin
Plasmid#136573PurposeHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)DepositorInsertemiRFP670-tubulin
TagsemiRFP670ExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLANT-2b
Plasmid#231487PurposeExpresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.DepositorInsertsTagsHis6 tag and His6-SUMO^NITExpressionBacterialMutationDeleted amino acids 1-6 (MEPEES). and M83L, E84Q,…PromoterT7 promoterAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM6-E12-vioABECD
Plasmid#66537PurposepETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)DepositorInsertsVioA
VioB
VioE
VioC
VioD
UseSynthetic BiologyExpressionBacterialPromoterMutant T7 Promoter - G6 and Mutant T7 Promoter - …Available SinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)
Plasmid#86419PurposeC-terminal PCR tagging module for yeast codon optimized split GFP1-10DepositorInsertGFP1-10
ExpressionYeastAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCfB2797
Plasmid#63639PurposepCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-FLEX-eNpHR-EYFP
Plasmid#89876PurposeTo generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoterDepositorInserteNpHR=EYFP
UseAAVTagsEYFPExpressionMammalianPromoterTetOAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only