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19,206 results

Showing: 4411 - 4440 of 19206 results

  1. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid
    #105556
    Purpose
    AAV expression of EGFP from tMCK promoter
    Depositor
    James M. Wilson
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tMCK
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105556-plasmid-map-sequence-id-202405

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  3. pTP955

    Plasmid
    #104164
    Purpose
    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)
    Depositor
    Dirk Görlich
    Article
    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154
    Insert
    Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
    Use
    Tags
    14xHistidine tag and NEDD8 from Brachypodium dist…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    104164-plasmid-map-sequence-id-322877

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  4. AICSDP-36: AAVS1-mEGFP (PGK)

    Plasmid
    #114404
    Purpose
    Homology arms and linker-mEGFP sequence for internal insertion of PGK-mEGFP at the AAVS1 safe harbor location in human cells as a reporter for cytoplasmic mEGFP
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with PGK driven mEGFP (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114404-plasmid-map-sequence-id-220815

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  6. pCMV-15F11-HA-mCh

    Plasmid
    #129591
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mCherry (15F11-HA scFv-mCh)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    129591-plasmid-map-sequence-id-254269

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  8. pOpen_taq

    Plasmid
    #153315
    Purpose
    Bacterial expression of Taq polymerase
    Depositor
    Drew Endy
    Insert
    Taq polymerase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tac
    Available since
    June 2, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153315-plasmid-map-sequence-id-298774

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  14. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  15. pKAM-BFP-Puro

    Plasmid
    #101864
    Purpose
    Lentiviral empty backbone, dual BFP and puro markers
    Depositor
    Archibald Perkins
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    May 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    101864-plasmid-map-sequence-id-211112

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  21. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)
    Use
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70112-plasmid-map-sequence-id-418643

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  22. pCDH-EF1-Nluc-P2A-copGFP-T2A-Puro

    Plasmid
    #73022
    Purpose
    Luminescent and fluorescent dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nluc-P2A-copGFP-T2A-Puro
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1
    Available since
    Jan. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    73022-plasmid-map-sequence-id-136024

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  25. pHH21-BNA-Luc

    Plasmid
    #187924
    Purpose
    Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells
    Depositor
    Arindam Mondal
    Article
    Kedia et al Front Microbiol. 2022 May 25;13:868367.
    Insert
    Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymease I Promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    187924-plasmid-map-sequence-id-373471

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  27. pDestTol2CG2-U6:gRNA

    Plasmid
    #63156
    Purpose
    Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.
    Depositor
    Leonard Zon
    Article
    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15
    Type
    Empty backbone
    Use
    CRISPR; Tol2 destination vector for gateway
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    63156-plasmid-map-sequence-id-177022

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  30. pP{ELAV-GeneSwitch}

    Plasmid
    #83957
    Purpose
    Expresses conditional RU486-dependent GAL4 protein under control of the elav promotor
    Depositor
    Haig Keshishian
    Article
    Osterwalder et al Proc Natl Acad Sci U S A. 2001 Oct 23;98
    Insert
    fusion protein consisting of the GAL4-DBD, human progesterone receptor-ligand-binding domain, and P65-AD (GAL4, RELA Budding Yeast, Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    elav
    Available since
    Dec. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    83957-plasmid-map-sequence-id-160516

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Showing: 4411 - 4440 of 19206 results