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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pNCST-AausGFP

Plasmid

#129503

Purpose

Expresses AausGFP constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

AausGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMito-miRFP703

Plasmid

#80000

Purpose

Mitochondria labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

cytochrome C oxidase subunit VIII (COX8A Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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ppk301-T2A-QF2 HDR plasmid

Plasmid

#130667

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti ppk301 gene

Depositor

Leslie Vosshall

Article

Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

Insert

ppk301-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; ppk301-right-HDR-arm

Use

Donor template

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-FLEX-tTA

Plasmid

#178516

Purpose

AAV vector for Cre recombinase dependent tetracycline-controlled transactivator (tTA) expression

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

tTA

Use

AAV

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A24

Plasmid

#135507

Purpose

Mammalian expression of myc-tagged HLA-A*024:02

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*24:02 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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araBAD-BCD20-ClpP-BCD17-ClpX

Plasmid

#153301

Purpose

Expresses clpX and clpP in E.Coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Inserts

clpX (clpX Escherichia coli)
clpP (clpP Escherichia coli)

Expression

Bacterial

Promoter

araBAD

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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IMPT-10314:GABBR2

Plasmid

#157851

Purpose

HA-3xFLAG-VFT-TMD-CC in pFastBac1 for Sf9 expression

Depositor

Vadim Cherezov, Raymond Stevens

Article

Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

Insert

GABBR2 (GABBR2 Human)

Expression

Insect

Promoter

Polyhedrin

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pUAST attB Orco (Y478A)

Plasmid

#73928

Purpose

UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

Depositor

Leslie Vosshall

Article

Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

Insert

Orco (Y478A) (Orco Fly)

Use

Unspecified

Mutation

changed 478 Tyrosine for Alanine

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight2-VP16-T2A-mTagBFP2

Plasmid

#219548

Purpose

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

NLS-Gal4(1-65)-iLight2-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

The (SAGG)x4 linker between GAL4(1-65) and the PC…

Promoter

CMVd1

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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EXT1-pLJC2-3XFLAG

Plasmid

#215818

Purpose

Ectopic expression of EXT-1 in mammalian cells

Depositor

Yoav Shaul

Article

Solaimuthu et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

Exostosin glycosyltransferase 1 (EXT1 Human)

Use

Lentiviral

Tags

3X Flag

Expression

Mammalian

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pNCST-avGFP

Plasmid

#129508

Purpose

Expresses avGFP (Lambert 2019 clone) constitutively in E. coli (most strains)

Depositor

Nathan Shaner

Article

Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

Insert

avGFP

Expression

Bacterial

Promoter

synthetic constitutive (stationary phase) promote…

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_NRL_P2A_Hygro_Barcode

Plasmid

#120469

Purpose

Barcoded lentiviral vector to express NRL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NRL (NRL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLVH-EF1a-GFP-P2A-MYC

Plasmid

#130695

Purpose

Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

Depositor

Kejin Hu

Article

Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

Insert

human MYC (MYC Human)

Use

Lentiviral

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

Plasmid

#218862

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8m

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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mOrange2-Fibrillarin-7

Plasmid

#57957

Purpose

Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Fibrillarin (FBL Human)

Tags

mOrange2

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
DPYSL2-pLJC2

Plasmid

#189866

Purpose

Expresses human DPYSL2 (CRMP2) in mammalian cells

Depositor

Yoav Shaul

Article

Abu Rmaileh et al J Cell Biol. 2022 Jul 4;221(7). pii: 213

Insert

DPYSL2 (DPYSL2 Human)

Use

Lentiviral

Tags

3X FLAG

Expression

Mammalian

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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