We narrowed to 9,441 results for: pho

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

minimal CMV promoter with tetracycline operator

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCV-Flt3-Y591F/Y919F

Plasmid

#74498

Purpose

Retroviral expression of FLT3 Y591F/Y919F

Depositor

Lars Rönnstrand

Article

Kazi et al J Biol Chem. 2012 Oct 19;287(43):36509-1

Insert

FLT3 (FLT3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Y591F/Y919F, T227M

Promoter

unknown

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag Grb10 9A long

Plasmid

#37485

Depositor

David Sabatini

Article

Hsu et al Science. 2011 Jun 10;332(6035):1317-22.

Insert

Grb10 9A (GRB10 Human)

Tags

Flag

Expression

Mammalian

Mutation

isoform A, 594aa, S104A S150A T155A S158A S426A S…

Available Since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Drosha1-390

Plasmid

#62521

Purpose

C-terminal aa391-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 391-1374 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 391-1374

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-Cx43-YA/VD-GFP

Plasmid

#60703

Purpose

mammalian expression of rat Cx43 YA/VD mutant fused to GFP

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Tags

EGFP

Expression

Mammalian

Mutation

YA/VD (Y286A/V289D)

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pRevTRE MKL1-N100 S449A/T450A/S454A

Plasmid

#19849

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1-N100 S449A/T450A/S454A (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Mutation

deleted amino acids 1-100 and changed Serine 449,…

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-hALDOA D34S

Plasmid

#210810

Purpose

To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 34 of ALDOA mutated to serine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX 4t1 RARalpha (1-187)

Plasmid

#35558

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Mutation

aa 1-187

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pGEX Tyk2-SH2

Plasmid

#46527

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tyk2 (TYK2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 452-567

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TDP-43_NTD1-80_Y4R

Plasmid

#104478

Purpose

bacterial expression of TDP-43 NTD Y4R

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

His

Expression

Bacterial

Mutation

Y4R

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pX131

Plasmid

#167149

Purpose

MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

Depositor

Karen Sarkisyan

Article

Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

Insert

4'-phosphopantetheinyl transferase, npgA

Use

Synthetic Biology

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX304-ARF5-mNeonGreen

Plasmid

#162025

Purpose

Expression of tagged ARF WT

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Insert

ARF5 (ARF5 Human)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-WT

Plasmid

#91986

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX 4T1 RARalpha (186-462)

Plasmid

#35559

Depositor

Jonathan Kurie, Harish Srinivas

Article

Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

Insert

RARalpha (RARA Human)

Tags

GST

Expression

Bacterial

Mutation

aa 186-462

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only
pT7-7 asyn S87E

Plasmid

#36059

Depositor

Hilal Lashuel

Article

Paleologou et al J Neurosci. 2010 Mar 3;30(9):3184-98.

Insert

alpha-synuclein S87E (SNCA Human)

Expression

Bacterial

Mutation

S87E

Promoter

T7

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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hHSF1 S303A

Plasmid

#118363

Purpose

This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

Depositor

Lea Sistonen

Article

Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

Insert

hHSF1 (HSF1 Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

S303A

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha1-850

Plasmid

#62522

Purpose

C-terminal aa851-1374 of Drosha is deleted

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha with aa 851-1374 deleted (DROSHA Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 851-1374

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pTER GAMMA-TUBULIN shRNA Human

Plasmid

#87955

Purpose

reduced the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

sh gamma-tubulin (TUBG1 Human)

Tags

No-tag

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene.…

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 S449A/T450A/S454A

Plasmid

#19845

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A/T450A/S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449, Threonine 450 and Serine 454 …

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only