We narrowed to 23,602 results for: CRISPR

Showing: 4441 - 4470 of 23602 results

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pCas9-polyA

Plasmid

#72602

Purpose

express hCas9 byT7 promoter with polyadenine tails

Depositor

Tomoji Mashimo

Article

Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

Insert

Cas9-polyadenine

Tags

81-bp polyadenylation

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA2 TKIT

Plasmid

#169442

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-RSV-SpCas9

Plasmid

#85450

Purpose

Express SpCas9 in mammalian cells

Depositor

Hetian Lei

Article

Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

Insert

pRSV

Use

AAV

Promoter

pRSV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA2 TKIT donor

Plasmid

#169440

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pJYScr

Plasmid

#132719

Purpose

Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

Depositor

Jan Marienhagen

Article

Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

Plasmid

#196828

Purpose

Express dpdpCas13b with AAAA mutation

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

dpspCas13b(AAAA)

Tags

3XFLAG and NES

Expression

Mammalian

Mutation

K367A, D369A and K370A

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA2 TKIT donor

Plasmid

#169421

Purpose

Donor DNA for TKIT knockin of SEP to GluA2 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XMS2

Plasmid

#75389

Purpose

sgRNA1-2XMS2

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pem7 and Pm

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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Anep8_Cas9

Plasmid

#117169

Purpose

Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

Depositor

Adrian Tsang

Article

Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Filamentous fungi e…

Promoter

Pki

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluN1 TKIT donor

Plasmid

#169427

Purpose

Donor DNA for TKIT knockin of SEP to NR1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pHFS:SEP-rGluA1 TKIT donor

Plasmid

#169438

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in rat

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-Cas9

Plasmid

#62209

Purpose

Expresses Cas9 under control of act5C promoter and SV40 3'UTR. For ubiquitous genome engineering in Drosophila melanogaster.

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

cas9

Expression

Insect

Promoter

act5C

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-SEP-GluA1 TKIT

Plasmid

#169441

Purpose

Expression of 2 guides + donor DNA

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

AAV and CRISPR

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141B

Plasmid

#69291

Purpose

Gateway entry vector; single gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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NM1-5S-tRNA-SgH

Plasmid

#128178

Purpose

Expresses gRNA using fusion of R. toruloides 5S rRNA and tRNA-Arg as promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

gRNA cloning cassette

Use

CRISPR

Expression

Yeast

Promoter

5S rRNA-tRNA(Arg)

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141C

Plasmid

#69292

Purpose

Gateway entry vector; single gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pJJB308

Plasmid

#107699

Purpose

AtU6 Promoter-LwaCas13a-gRNA Scaffold

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Type

Empty backbone

Expression

Plant

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Article

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available Since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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pX330A-1x6

Plasmid

#58770

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pXT128_TwinStrep-SUMO-ShCas12k

Plasmid

#135524

Purpose

Protein expression construct for ShCast12k

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShCas12k

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ141A

Plasmid

#69290

Purpose

Gateway entry vector; single gRNA under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMLS485

Plasmid

#188890

Purpose

Worm Cas9 expression vector, Peft-3 promoter

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Peft-3::Cas9

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PP7 guide

Plasmid

#86168

Purpose

Expresses S. Pyogenes guide with PP7 aptamer in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

PP7 guide

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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gRNA Cloning Vector Bbs I ver. 2

Plasmid

#85586

Purpose

An empty sgRNA expression vector. sgRNA sequence can be cloned into its Bbs I site. Its gRNA scaffold has efficient ver. 2 structure.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2016 Jul 28;6:30485. doi: 10.10

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pBx-Spas-sgRNA-Gm

Plasmid

#105232

Purpose

Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Gentamycin marker for P. aeruginosa

Depositor

Kristala Prather

Article

Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

Insert

S. pasteurianus sgRNA

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-hph-dCas9

Plasmid

#218371

Purpose

Expression of dCas9 for CRISPR-interference

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Promoter

PtetA

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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