2,226 results

1. AAV-SYP1-miniSOG-citrine

   Plasmid

   #50971

   Purpose

   AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   Synaptophysin-miniSOG-citrine (Syp Rat)
   

   Use

   AAV

   Tags

   citrine and miniSOG

   Expression

   Mutation

   Promoter

   human synapsin promoter

   Available since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-mEGFP(A206K)-paCaMKII-ShadowG

   Plasmid

   #167475

   Purpose

   Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

   Depositor

   Hideji Murakoshi

   Article

   Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

   Insert

   mEGFP(A206K)-paCaMKII-ShadowG
   

   Use

   Tags

   ShadowG and mEGFP(A206K)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Syn-ATP

   Plasmid

   #51819

   Purpose

   Optical reporter of presynaptic ATP

   Depositor

   Timothy Ryan

   Article

   Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

   Insert

   Chimeric Synaptophysin-mCherry-Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Within WT luciferase gene, changed Threonine 214 …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEos2-PSD95-N-14

   Plasmid

   #57411

   Purpose

   Localization: Membrane Scaffold, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Michael Davidson

   Insert

   PSD95
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 5, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pMXs-puro GFP-b5 ER

   Plasmid

   #38274

   Depositor

   Noboru Mizushima

   Article

   Itakura et al Autophagy. 2010 Aug;6(6):764-76.

   Insert

   Cytochrome b5 (Cyb5a Rat)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Cyb5a Tranmembrane region from 95 to 134 amino ac…

   Promoter

   Available since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. GFP-ERK2

   Plasmid

   #37145

   Depositor

   Rony Seger

   Article

   Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

   Insert

   ERK2 (MAPK1 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa1-6 deleted as a result of cloning strategy

   Promoter

   CMV

   Available since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

7. mCardinal-Lysosomes-20

   Plasmid

   #56166

   Purpose

   Localization: Lysosome Membrane, Excitation: 604, Emission: 659

   Depositor

   Michael Davidson

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   LAMP1
   

   Use

   Tags

   mCardinal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

8. FUGW-VGAT-pHluorin

   Plasmid

   #207467

   Purpose

   Lentiviral expression of VGAT-pHluorin

   Depositor

   Robert Edwards

   Article

   Jain et al bioRxiv 2023

   Insert

   VGAT (Slc6a12 Rat)
   

   Use

   Lentiviral

   Tags

   pHluorin

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-hSyn-Axon-jGCaMP8m

   Plasmid

   #172719

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE unc13a GFP KI

    Plasmid

    #131498

    Purpose

    Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Unc13a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Barr2 flag

    Plasmid

    #14685

    Depositor

    Robert Lefkowitz

    Article

    Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

    Insert

    beta-arrestin 2 (Arrb2 Rat)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

12. C1A-C1A-EYFP

    Plasmid

    #61155

    Purpose

    Diacylglycerol (DAG) reporter

    Depositor

    Tobias Meyer

    Article

    Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

    Insert

    C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C1A from PKCG amino acids 26-89

    Promoter

    CMV

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-RatLC3B-C-7

    Plasmid

    #55128

    Purpose

    Localization: Microtubule-Binding Protein, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    LC3B
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRK7-HA-S6K1-E389-deltaCT

    Plasmid

    #8993

    Depositor

    John Blenis

    Article

    Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T389E, stop codon at amino acid 402

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. tdTomato-KIF1A

    Plasmid

    #102850

    Purpose

    Overexpresses KIF1A and cell fill TdTomato

    Depositor

    Erik Dent

    Article

    McVicker et al Nat Commun. 2016 Sep 23;7:12741. doi: 10

    Insert

    KIF1A (Kif1a Rat)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET42b-HF-BE3

    Plasmid

    #87438

    Purpose

    Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

    Insert

    HF-BE3 (Apobec1 Bacillus phage PBS2, Human, Rat)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. VAChT-pHuji

    Plasmid

    #105319

    Purpose

    VAChT tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAChT (Slc18a3 Rat)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Camui-CR

    Plasmid

    #40256

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Camuiα-CR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-28a-His6-SUMO-Munc18-1wt

    Plasmid

    #135550

    Purpose

    Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18-1wt
    

    Use

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Mutation

    No ATG start codon

    Promoter

    T7 promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

    Plasmid

    #38262

    Depositor

    Robert Lefkowitz

    Article

    Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

    Insert

    β-arrestin2 (Arrb2 Rat)
    

    Use

    Tags

    Flag and PIP5K Iα core kinase domain (residues 18…

    Expression

    Mammalian

    Mutation

    Arg 233, Arg237, and Lys251 converted to Glutamin…

    Promoter

    CMV

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCR3-FLAG-Gephyrin P1

    Plasmid

    #68816

    Purpose

    Flag tagged Geph mammalian expression

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    silent mutations on internal EcoRI site. Gephyrin…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

23. vGAT-pH pcaggs SE

    Plasmid

    #78578

    Purpose

    image recycling of VGAT in live neurons

    Depositor

    Susan Voglmaier

    Article

    Santos et al J Neurosci. 2013 Jun 26;33(26):10634-46.

    Insert

    vGAT-pHluorin C-term (Slc32a1 Rat)
    

    Use

    Tags

    superecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    chicken actin

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

24. pMK294 (mAID-mCherry2-Bsr)

    Plasmid

    #72832

    Purpose

    C-terminal tagging with mAID-mCherry2 using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mCherry2-Bsr
    

    Use

    CRISPR

    Tags

    mAID-mCherry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. MUNC18a

    Plasmid

    #218888

    Purpose

    Expresses rat MUNC18a in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    Munc18a (Stxbp1 Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. G protein alpha-s(alpha3beta5/G226A/A366S)

    Plasmid

    #55797

    Purpose

    This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

    Depositor

    Catherine Berlot

    Article

    Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

    Insert

    alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
    

    Use

    Tags

    internal EE epitope (residues 189-194 in alpha-s …

    Expression

    Mammalian

    Mutation

    N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

    Promoter

    CMV

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pZac2.1 GfaABC1D rM3D mCherry SV40

    Plasmid

    #92285

    Purpose

    AAV DREADD expression

    Depositor

    Baljit Khakh

    Article

    Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

    Insert

    rM3D
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    GfaABC1D

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFudio-Cpg15-IRES2-TdTomatoW

    Plasmid

    #134309

    Purpose

    Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

    Depositor

    Elly Nedivi

    Article

    Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

    Inserts

    cpg15 (Nrn1 Mouse, Rat)
    TdTomato
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. His-GFP-Rim2

    Plasmid

    #170869

    Purpose

    Mammalian expression of His-GFP-Rim2.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. mCit-4aa-SNAP25B

    Plasmid

    #53235

    Purpose

    Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    SNAP-25B (Snap25 Rat)
    

    Use

    Tags

    4aa-linker and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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