We narrowed to 2,735 results for: SAB

1. pLJC5-LAMP1-RFP-3xHA

   Plasmid

   #102932

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-HA)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   RFP-HA

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLJC5-LAMP1-RFP-2xFlag

   Plasmid

   #102931

   Purpose

   Lentiviral expression of a lysosomal tag (LAMP1-mRFP1-Flag)

   Depositor

   David Sabatini

   Article

   Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

   Insert

   LAMP1
   

   Use

   Lentiviral

   Tags

   mRFP-2xFlag

   Promoter

   ubc

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-Cas9-GFP

   Plasmid

   #86145

   Purpose

   Lentiviral vector expressing Cas9-GFP

   Depositor

   David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Insert

   Cas9-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-TRE-DIO-ChR2-TdTomato

   Plasmid

   #166600

   Purpose

   Encodes Cre-dependent ChR2-TdTomato under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   ChR2-TdTomato
   

   Use

   AAV

   Mutation

   H134R

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcw107

   Plasmid

   #62511

   Purpose

   Gateway Expression Vector

   Depositor

   David Sabatini, John Doench

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. myc-Rictor corrected

   Plasmid

   #11367

   Purpose

   Mammalian expression of myc tagged human RICTOR

   Depositor

   David Sabatini

   Article

   Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

   Insert

   Rictor (RICTOR Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-TRE-DIO-eGFP-2A-TetanusToxin

   Plasmid

   #166603

   Purpose

   Encodes Cre-dependent eGFP-2A tetanus toxin light chain fragment under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIS1-Eef25UTR-renilla

   Plasmid

   #38235

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2 5'UTR / Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Eef2 5' UTR fused to renilla ORF

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pIS1-Eef25UTR-TOPmut-renilla

   Plasmid

   #38236

   Depositor

   David Sabatini

   Article

   Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

   Insert

   Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   The first nucleotides of the 5' UTR were cha…

   Promoter

   Endogenous Eef2

   Available Since

   Nov. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pCW57.1-4EBP1_4xAla

    Plasmid

    #38240

    Depositor

    David Sabatini

    Article

    Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

    Insert

    4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    T37A, T46A, S65A, T70A (dominant negative)

    Promoter

    Tet ON

    Available Since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Snap-Omp25

    Plasmid

    #69599

    Purpose

    Snap-tag substrates with two different fluorophores (red and green) sequentially to separately label young and old mitochondria

    Depositor

    David Sabatini

    Article

    Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

    Insert

    Snap-Omp25
    

    Expression

    Mammalian

    Available Since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs-DDX3X

    Plasmid

    #70648

    Purpose

    Expresses WT DDX3X in mammalian cells under blasticidin selection.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Retroviral

    Tags

    3x FLAG

    Expression

    Mammalian

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

    Plasmid

    #37082

    Purpose

    Cre-dependent expression of hChR2(H134R) for optogenetic excitation

    Depositor

    Bernardo Sabatini

    Article

    Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

    Insert

    hChr2(H134R)-mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    mCherry

    Promoter

    EF1a

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs_FLAG-NMNAT1

    Plasmid

    #133259

    Purpose

    Express FLAG-tagged NMNAT1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NMNAT1 (NMNAT1 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRK5-HA-metap2

    Plasmid

    #100512

    Purpose

    Expresses HA tagged metap2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    Metap2 (METAP2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRK5 FLAG metap2

    Plasmid

    #32004

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    metap2 (Metap2 )
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO mouse shRNA 1 rictor

    Plasmid

    #21341

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    rictor shRNA 1 (Rictor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag Nprl2 pRK5

    Plasmid

    #46333

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRK5-FLAG-SAMTOR

    Plasmid

    #100508

    Purpose

    Expresses FLAG tagged SAMTOR in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

    Plasmid

    #166610

    Purpose

    Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    ChR2-TdTomato
    

    Use

    AAV

    Mutation

    H134R

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pETDuet RagA-RagC

    Plasmid

    #99641

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AICSDP-63: ACTN2-mEGFP

    Plasmid

    #124607

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Insert

    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. myc-Rictor

    Plasmid

    #1860

    Purpose

    Please see Addgene item #11367 for an updated version of this plasmid.

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    June 20, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

    Plasmid

    #166611

    Purpose

    Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    EGFP-2A-Tetanus Toxin
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFloat.cspA_v1

    Plasmid

    #133071

    Purpose

    Entry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    cspA

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. myc-mTOR (1750-2549 aa)

    Plasmid

    #21747

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1750-2549) (Mtor Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1750-2549

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pMXs-RAP2A-GFP

    Plasmid

    #86124

    Purpose

    Retroviral vector expressing RAP2A-GFP-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Retroviral

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. myc-mTOR (1271-2008 aa)

    Plasmid

    #21746

    Depositor

    David Sabatini

    Article

    Kim et al Mol Cell 2003 Apr;11(4):895-904.

    Insert

    mTOR (1271-2008) (Mtor Rat)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    amino acids 1271-2008

    Available Since

    Aug. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

31. FLAG-GK in PLJM60

    Plasmid

    #154207

    Purpose

    Lentiviral vector for stable expression of GK

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    GK (GK Human)
    

    Use

    Lentiviral

    Tags

    N terminal FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

    Plasmid

    #166609

    Purpose

    Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAAV-Ef1a-DO-EGFP-WPRE-pA

    Plasmid

    #37085

    Purpose

    Expresses EGFP in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox; Cre-off

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLKO mouse shRNA 1 raptor

    Plasmid

    #21339

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    raptor shRNA 1 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

35. TPI in pCW57_tTA_Blast

    Plasmid

    #154209

    Purpose

    Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    TPI1 (TPI1 Human)
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE repeats (dox off)

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV hSyn grpLight1.2

    Plasmid

    #175174

    Purpose

    genetically encoded GRP sensor grpLight1.2

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    hSyn-driven grpLight1.2
    

    Use

    AAV

    Available Since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAAV-Ef1a-DO-mCherry-WPRE-pA

    Plasmid

    #37119

    Purpose

    Expresses mCherry in cells lacking Cre (Cre-Off)

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox; Cre-off

    Promoter

    EF1a

    Available Since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5-HA-SAMTOR (D190A)

    Plasmid

    #100515

    Purpose

    Expresses HA tagged SAMTOR D190A mutant in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    SAMTOR (BMT2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D190A

    Available Since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXs-NDUFAF5-HA

    Plasmid

    #86125

    Purpose

    Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    NDUFAF5 (NDUFAF5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMSCV mLST8 myc (aka: GbL-myc)

    Plasmid

    #12580

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mLST8 (MLST8 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only