We narrowed to 12,214 results for: LEA
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Plasmid#98184PurposeLow Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLDLR (LDLR Human)
TagsGFP and LDLR signal sequence, N-term of GFP so GF…ExpressionMammalianMutationnonePromoterChicken Beta ActinAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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BPK3079
Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-mCherry-IRES-Flpo
Plasmid#55634PurposeExpresses Flpo in Mammalian CellsDepositorHas serviceAAV1 and AAV RetrogradeInsertsmCherry
Flpo
UseAAVExpressionMammalianPromoterEf1a and Ef1a/IRESAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Dominant negative Hsp90 beta
Plasmid#22480DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDominant negative Hsp90 (HSP90AB1 Human)
TagsHAExpressionMammalianAvailable sinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pY002 (pFnCpf1_min)
Plasmid#69975PurposeExpresses FnCpf1 and spacers 1-4 of CRISPR array under artificial promoter. Cas genes are removed.DepositorInsertFnCpf1 locus without Cas genes nd artificial promoter
UseCRISPRExpressionBacterialAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMLM3705
Plasmid#47754PurposeExpresses mammalian cell codon-optimized dCas9-VP64DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcodon optimized dCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9 (catalytically i…PromoterCMVAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialPromoterT7-lacO (lactose/IPTG inducible)Available sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-RAM-d2TTA::TRE-NLS-mKate2-WPREpA
Plasmid#84474PurposepAAV-RAM-NLS-mKate2DepositorInsertmKate2
UseAAVTagsNuclear localization signal of SV40 large T antig…PromoterTREAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-HA2-DDB1
Plasmid#19909DepositorPublicationInsertDamage-specific DNA binding protein 1 (DDB1 Human)
TagsHAExpressionMammalianMutationplease see depositor comment belowAvailable sinceApril 1, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-T3-hCAS-pA
Plasmid#48625PurposeExpresses CAS9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCodon optimized Cas9
UseCRISPRTagsFLAG, NLS, and polyAExpressionMammalianPromoterCAG promoterAvailable sinceNov. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBiFC-CrN173
Plasmid#22016DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCrN173
TagsFLAGExpressionMammalianMutationN/AAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET22b RNase A
Plasmid#58903PurposeBacterial expression of bovine pancreatic ribonuclease ADepositorInsertRNase A (RNASE1 Bovine)
TagspelB sequenceExpressionBacterialMutationSee Depositor Comments belowPromoterT7Available sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-DsRed_IRES_EGFP
Plasmid#92194PurposeLentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screenDepositorInsertDsRed-IRES-EGFP
UseLentiviralExpressionMammalianAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus
Plasmid#61629PurposeR2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoterDepositorInsertsmDII
ntdTomato
DII
n3xVenus
ExpressionPlantPromoterRPS5AAvailable sinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBad-mKalama1
Plasmid#14892PurposeBacterial expression of mKalama1 blue fluorescent proteinDepositorInsertmKalama1
TagsHisExpressionBacterialMutationThe fluorescent protein was inserted between XhoI…Available sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by