We narrowed to 5,960 results for: actin

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Actin binding domain of Ezrin

Plasmid

#62381

Purpose

Expresses the actin binding domain of ezrin. Amino acids 552-586.

Depositor

Guillaume Charras

Article

Fritzsche et al Biophys J. 2014 Jan 21;106(2):343-53. do

Insert

Actin binding domain of ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

contains aa's 552-586

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-GS1

Plasmid

#89445

Purpose

Expresses EGFP-GS1 (Human Gelsolin 53-176) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-GS1 (GSN Human)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning cytoplasmic Gsn amino acids 5…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCXWB-EBNA1

Plasmid

#37624

Purpose

Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

Depositor

Shinya Yamanaka

Article

Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

Insert

EBNA-1 (EBNA-1 human herpesvirus 4)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pGTag-gal4-VP16-B-actin

Plasmid

#117817

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

gal4/VP16

Use

Synthetic Biology

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Lifeact-7

Plasmid

#54586

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

Lifeact-mTagRFP-T

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-loxPSTOPloxP-ZsGreen

Plasmid

#51269

Purpose

Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

loxP-STOP-loxP-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_AlphaSMA_ALFA_231232

Plasmid

#250441

Purpose

Expresses tagged alpha smooth muscle actin in mammalian cells. Protein is tagged with an internal ALFA tag at position T231/A232.

Depositor

Koen van den Dries

Article

van Zwam et al PLoS Biol. 2024 Mar 11;22(3):e3002551. d

Insert

Alpha Smooth Muscle Actin (ACTA2 Human)

Tags

ALFA Tag (Internal)

Expression

Mammalian

Promoter

CMV Promotor

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-R62D

Plasmid

#118379

Purpose

Gateway compatible donor vector with actin-R62D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

R62D

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-DeAct-SpvB

Plasmid

#89446

Purpose

Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

Depositor

Brad Zuchero

Article

Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

Insert

DeAct-SpvB (spvB Salmonella enterica)

Tags

EGFP

Expression

Mammalian

Mutation

Truncation spanning SpvB amino acids 375-591 (mon…

Promoter

CMV

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Flpe

Plasmid

#13787

Purpose

Mammalian expression of enhanced form of Flp recombinase with C-terminal Myc tag

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Insert

Flpe

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

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pB-CAG-FLEX-F14F15-BFP-(mCherry)'

Plasmid

#201831

Purpose

piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

Depositor

Ian Wickersham

Article

Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Inserts

CAG
BFP

Expression

Mammalian

Promoter

CAG

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pFX-Lifeact-EGFP

Plasmid

#174477

Purpose

Express EGFP with actin-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with actin-targeting sequence

Tags

Lifeact (N-terminal 17 amino acids from S. Cerevi…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-T7pol

Plasmid

#59926

Purpose

Expression vector for T7 RNA polymerase gene

Depositor

Ian Wickersham

Insert

T7 RNA polymerase

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Tuba

Plasmid

#22214

Depositor

Pietro De Camilli

Article

Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

Insert

Tuba (DNMBP Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 MCS-BirA(R118G)-HA

Plasmid

#36047

Purpose

To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); HA tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA(R118G)-HA

Tags

HA

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Available Since

April 13, 2012

Availability

Academic Institutions and Nonprofits only

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EYFP-NLS-actin-K68/284R

Plasmid

#60616

Purpose

Encodes YFP and NLS tagged beta-actin with sumoylation resistant mutations

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Mutation

K68R, 284R

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pC015 - dLwCas13a-NF

Plasmid

#91905

Purpose

Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

dLwCas13a

Use

CRISPR

Tags

NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

Expression

Mammalian

Mutation

R474A and R1046A

Promoter

CAG

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 mycBioID

Plasmid

#35700

Purpose

To fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tag

Depositor

Kyle Roux

Article

Roux et al J Cell Biol. 2012 Mar 12.

Has Service

Cloning Grade DNA

Insert

BirA

Tags

Myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

cmv

Available Since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab11a-7

Plasmid

#56444

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab11a (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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