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We narrowed to 16,039 results for: grna

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Showing: 281 - 300 of 16039 results

  3. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60621-plasmid-map-sequence-id-208224

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  8. pdCas9-Tet1-CD

    Plasmid
    #83340
    Purpose
    To introduce dCas9-fused Tet1-CD and sgRNA2.0 system
    Depositor
    Ronggui Hu
    Publication
    Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    U6, CBH
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83340-plasmid-map-sequence-id-334608

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  10. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Publication
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60847-plasmid-map-sequence-id-197511

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  12. pX330 p53

    Plasmid
    #59910
    Purpose
    pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter
    Depositor
    Tyler Jacks
    Publication
    Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting p53 (Trp53 Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59910-plasmid-map-sequence-id-516810

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  19. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Publication
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway Cloning
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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Showing: 281 - 300 of 16039 results