We narrowed to 1,562 results for: sgrna vector

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pX459-sgRNA2-RPB1-N-term

Plasmid

#195111

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA2-2-RPB1-C-term

Plasmid

#195109

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against last exon of RPB1 (C-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA4-CTCF-3p-UTR

Plasmid

#195106

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA3-CTCF-3p-UTR

Plasmid

#195105

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against CTCF 3' UTR region

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX459-sgRNA3-RPB1-N-term

Plasmid

#195112

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-sgRNA1-RPB1-N-term

Plasmid

#195110

Purpose

Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

Depositor

Job Dekker

Article

Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

Insert

sgRNA against first exon of RPB1 (N-terminal)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSK-mU6-Cj-sgRNA-SV40-puro

Plasmid

#192128

Purpose

Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

Depositor

Yongming Wang

Article

Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

Insert

Cj-SgRNA scaffold

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-dSaCas9-pU6-sgRNA

Plasmid

#159174

Purpose

Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX330.puro sgRNA KGA Stop1

Plasmid

#110405

Purpose

CRISPR/Cas9 vector with sgRNA for glutaminase KGA isoform C-Terminal Knock-in and puromycin resistance

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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mU6-sgRNA-CLTA-mCherry

Plasmid

#240637

Purpose

Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

Depositor

James Nuñez

Article

Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

Insert

puro-T2A-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-pMecp2-SaCas9-pU6-sgRNA

Plasmid

#159173

Purpose

Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

SaCas9

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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Pb459-mU6-sgRNA-EF1a-PuroR

Plasmid

#195507

Purpose

piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

Puromycin

Use

CRISPR; Piggybac

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCF821-recipient_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211651

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF820-recipient_U6-sgRNA-EF1a-mCherry2

Plasmid

#211647

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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