165,971 results

1. RFP-HA-DAT

   Plasmid

   #90265

   Purpose

   Expresses RFP-HA-DAT in mammalian cells

   Depositor

   Alexander Sorkin

   Article

   Caltagarone et al Mol Cell Neurosci. 2015 Sep;68:120-30. d

   Insert

   DAT (dopamine transporter) (SLC6A3 Human)
   

   Expression

   Mammalian

   Mutation

   inserted HA tag (see YFP-HA-DAT)

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTO_CHMP4B_LAP

   Plasmid

   #101850

   Purpose

   Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

   Depositor

   Daniel Gerlich

   Article

   Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

   Insert

   Charged Multivesicular Body Protein 4B (CHMP4B Human)
   

   Use

   Gateway cloning, flp-frt recombination, tet-induc…

   Tags

   Localization and Affinity Purification (LAP) tag

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF3008

   Plasmid

   #144484

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CDX2 (CDX2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

   Plasmid

   #49771

   Purpose

   Level 1 hCas9 module

   Depositor

   Sophien Kamoun

   Article

   Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

   Insert

   35Sp::hCas9
   

   Use

   CRISPR

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBOBI-hPLD3-flag

   Plasmid

   #222640

   Purpose

   lentiviral vector for full length human PLD3 expression (with flag tag)

   Depositor

   David Nemazee

   Insert

   PLD3 (PLD3 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00025 (aka pCW-dCas9-Blast)

   Plasmid

   #195316

   Purpose

   Dox inducible lentiviral dead Cas9

   Depositor

   Raj Chari

   Insert

   dead Cas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Bacterial and Mammalian

   Mutation

   D10A; H840A

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEAK10-His-Myc-Kal9

   Plasmid

   #25441

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal9
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pEAK10-His-Myc-Kal5

   Plasmid

   #25440

   Depositor

   Betty Eipper

   Article

   Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

   Insert

   Kal5
   

   Tags

   His and Myc

   Expression

   Mammalian

   Available Since

   July 15, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pRosetta_v2

   Plasmid

   #136477

   Purpose

   Lentiviral GFP Reporter assay for determining appropriate dose of puromycin, blasticidin, and hygromycin

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

   Insert

   HygroR-T2A-BlastR-P2A-PuroR-F2A-EGFP
   

   Use

   Lentiviral

   Promoter

   PGK

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC.HA Vav2

    Plasmid

    #14554

    Depositor

    Joan Brugge

    Article

    Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. 12xMoonTag-12xSunTag-kif18b-24xPP7

    Plasmid

    #128606

    Purpose

    Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Inserts

    12xMoonTag-12xSunTag
    kinesin family member 18b (KIF18B Human)
    

    Expression

    Mammalian

    Promoter

    Pcmv

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Promoter

    EF1a

    Available Since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmNeonGreenHO-G

    Plasmid

    #127912

    Purpose

    a mammalian expression backbone vector for humanized mNeonGreen

    Depositor

    Isei Tanida

    Article

    Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

    Insert

    humanized mNeonGreen
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-NR2A

    Plasmid

    #17924

    Depositor

    Stefano Vicini

    Article

    Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

    Insert

    NMDAR2A (Grin2a Rat)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP/golgin-160

    Plasmid

    #180630

    Purpose

    Expression of WT golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160 (GOLGA3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-As-crRNA

    Plasmid

    #78956

    Purpose

    Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    June 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-EGFP-AID-BubR1

    Plasmid

    #47330

    Purpose

    for plasmid integration using the Flp-In system. AID at N-terminal

    Depositor

    Don Cleveland

    Article

    Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

    Insert

    BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B Human)
    

    Tags

    AID and GFP

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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