4,178 results

1. pBabe-c-myc-zeo

   Plasmid

   #17758

   Depositor

   Susan Lindquist

   Article

   Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

   Insert

   c-myc (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

   Plasmid

   #73037

   Purpose

   Express Nluc and copGFP dual reporter

   Depositor

   Kazuhiro Oka

   Insert

   Nluc-P2A-copGFP-T2A-Puro
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. VCP(R155H)-EGFP

   Plasmid

   #23972

   Depositor

   Nico Dantuma

   Article

   Tresse et al Autophagy. 2010 Feb 22. 6(2):.

   Insert

   Valosin containing protein (VCP Human)
   

   Tags

   6xHis, EGFP, and HA

   Expression

   Mammalian

   Mutation

   R155H

   Available Since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-G-GECO1.2

   Plasmid

   #32446

   Purpose

   Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Expression

   Mammalian

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Promoter

   CMV

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EBFP2-nuc

   Plasmid

   #36085

   Depositor

   Pantelis Tsoulfas

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-neo-cOVA

   Plasmid

   #25097

   Depositor

   Maria Castro

   Article

   Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

   Insert

   cytoplasmic ovalbumin
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-HRas

   Plasmid

   #18662

   Depositor

   Karel Svoboda

   Article

   Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

   Insert

   H-Ras (HRAS Human)
   

   Tags

   monomeric EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFUGW-H1 empty vector

   Plasmid

   #25870

   Purpose

   3rd generation lentiviral vector

   Depositor

   Sally Temple

   Article

   Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Aug. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3258 pCMV hUBC9 wt HA

   Plasmid

   #14438

   Depositor

   Peter Howley

   Article

   Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

   Insert

   UBC9 (UBE2I Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMVHA SUZ12

    Plasmid

    #24232

    Purpose

    Mammalian expression of human SUZ12 with HA tag

    Depositor

    Kristian Helin

    Article

    Pasini et al EMBO J. 2004 Oct 13. 23(20):4061-71.

    Insert

    SUZ12 (SUZ12 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-SARS-Spike

    Plasmid

    #145031

    Purpose

    Express SARS-CoV spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV Spike
    

    Tags

    C9 and CD5 signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 16, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDuet TDP43 WT

    Plasmid

    #27462

    Depositor

    Aaron Gitler

    Article

    Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Coexpression

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmScarlet_alphaTubulin_C1

    Plasmid

    #85045

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Alpha-Tubulin (TUBA1B Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-(BbsI)_CBh-Cas9-T2A-BFP

    Plasmid

    #64323

    Purpose

    Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-EBFP2

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C1-A20

    Plasmid

    #22141

    Depositor

    Yihong Ye

    Article

    Li et al Biochim Biophys Acta. 2008 Jun . 1783(6)

    Insert

    A20 (TNFAIP3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

    Plasmid

    #74009

    Purpose

    Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

    Depositor

    Filippo Del Bene

    Article

    Di Donato et al Genome Res. 2016 Mar 8.

    Inserts

    5x UAS
    Cas9
    T2A
    GFP
    U6 promoter
    

    Use

    CRISPR

    Available Since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-p53

    Plasmid

    #22725

    Depositor

    Shinya Yamanaka

    Article

    Hong et al Nature. 2009 Aug 27. 460(7259):1132-5.

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pINDUCER11 (miR-RUG)

    Plasmid

    #44363

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Available Since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. APEX2-GBP

    Plasmid

    #67651

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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