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Showing: 6001 - 6040 of 10227 results

  5. pET28a-λN-βLac

    Plasmid
    #162039
    Purpose
    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)
    Depositor
    Karen Polizzi
    Article
    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199
    Insert
    λN domain of the λ phage and a β-lactamase fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162039-plasmid-map-sequence-id-325526

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  6. pZF-BCB-PDZ

    Plasmid
    #127478
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-PDZ
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    Use
    Cell-free transcription–translation
    Tags
    alpha 1-syntrophin PDZ domain
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    127478-plasmid-map-sequence-id-248851

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  9. LovTAP N538E

    Plasmid
    #61431
    Purpose
    Expresses calmodulin binding protein-LovTAP N538E fusion protein in E. coli cells. LovTAP N358E is a optogenetic protein that can repress the Trp promoter in the presence of blue light.
    Depositor
    Tobin Sosnick
    Article
    Strickland et al Nat Methods. 2010 Aug;7(8):623-6. doi: 1
    Insert
    LovTAP N538E
    Use
    Tags
    CBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac operator
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. pET29b-AviTag-eGFP-dspB(E184Q)-6xHis

    Plasmid
    #175784
    Purpose
    Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
    Depositor
    Mark Nitz
    Article
    Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12
    Insert
    Dispersin B
    Use
    Tags
    AviTag, Hexahistidine tag, and eGFP
    Expression
    Bacterial
    Mutation
    E184Q
    Promoter
    T7
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    175784-plasmid-map-sequence-id-345284

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  23. Fast-GCaMP-RS15

    Plasmid
    #52652
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47/H48 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  39. SMT205

    Plasmid
    #134817
    Purpose
    SMT201 with mutated RBS for DHFR, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134817-plasmid-map-sequence-id-274011

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Showing: 6001 - 6040 of 10227 results