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We narrowed to 9,302 results for: BLI;

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Showing: 7551 - 7600 of 9302 results

  1. sgRNA(MS2)_zeo insert c-MYC

    Plasmid
    #164636
    Purpose
    sgRNA(MS2)_zeo backbone with cloned target sequence for human c-MYC. The insert sequence is: 5-CACCGTTCCCCCACGCCCTCTGCTT-3´ . This sgRNA achieves an upregulation of MYC in combination with dCAS9-VP64
    Depositor
    Günter Schneider
    Insert
    target sequence for c-Myc activation (MYC Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164636-plasmid-map-sequence-id-326094

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  2. TAF1

    Plasmid
    #39118
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. AICSDP-144:CTCF-mEGFP

    Plasmid
    #168799
    Purpose
    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human CTCF
    Depositor
    Allen Institute for Cell Science
    Insert
    CTCF (CTCF Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    June 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168799-plasmid-map-sequence-id-334694

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  4. pFP.R364

    Plasmid
    #138234
    Purpose
    Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138234-plasmid-map-sequence-id-273460

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  5. pFP.R274

    Plasmid
    #138223
    Purpose
    Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GSK).TvoVMA.(TVI)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138223-plasmid-map-sequence-id-296592

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  6. pcDNA3.1_hMed25

    Plasmid
    #206958
    Purpose
    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    206958-plasmid-map-sequence-id-422373

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  7. pALS3-Ma-SUMO-TAG35-sfGFP

    Plasmid
    #212120
    Purpose
    Fluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-TAG35-sfGFP and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-TAG35-sfGFP
    M. alvus Pyl-tRNA
    Tags
    His6 and SUMO-TAG35
    Expression
    Bacterial
    Mutation
    E35TAG (within SUMO encoding sequence)
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212120-plasmid-map-sequence-id-444184

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  10. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid
    #110376
    Purpose
    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110376-plasmid-map-sequence-id-213402

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  13. pME-hygro-SNAP-FLAG-CD59

    Plasmid
    #50379
    Purpose
    Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammalian cells
    Depositor
    Reika Watanabe
    Insert
    SNAP-FLAG-tagged CD59 (CD59 Human)
    Tags
    FLAG and SNAP
    Expression
    Mammalian
    Promoter
    SR alpha
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. AAV-SYN-TdTomato-RAB11

    Plasmid
    #203732
    Purpose
    AAV vector plasmid expressing human RAB11 fused to TdTomato under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    TdTomato
    Expression
    Mammalian
    Promoter
    Human synapsin promoter
    Available Since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203732-plasmid-map-sequence-id-402639

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  15. SMARCA4

    Plasmid
    #39116
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SMARCA4 (SMARCA4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. pFP.R275

    Plasmid
    #138224
    Purpose
    Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGK).PhoRadA.(TQL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138224-plasmid-map-sequence-id-273527

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  18. pFP.R371

    Plasmid
    #138241
    Purpose
    Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138241-plasmid-map-sequence-id-273486

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  19. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  20. pFP.R401

    Plasmid
    #138222
    Purpose
    Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IGC).TerThyX*.(SFD)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138222-plasmid-map-sequence-id-273515

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  21. pFP.R360

    Plasmid
    #138230
    Purpose
    Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HGK).Ama-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138230-plasmid-map-sequence-id-273492

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  22. pVRC8400-SARS-CoV-2-RBD-BA.4/5-AVI

    Plasmid
    #192114
    Purpose
    Expresses SARS-CoV-2 RBD protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Insert
    SARS-CoV-2-RBD-BA.4/5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G339D, S371F, S373P, S375F, T376A, D405N, R408S, …
    Available Since
    Oct. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    192114-plasmid-map-sequence-id-374985

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  24. HA-hDAGLb-V5

    Plasmid
    #87675
    Purpose
    Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal
    Depositor
    Pat Doherty
    Insert
    Human Diacylglycerol Lipase Beta (DAGLB Human)
    Tags
    HA and V5 tag
    Expression
    Mammalian
    Mutation
    Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…
    Promoter
    T7
    Available Since
    March 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    87675-plasmid-map-sequence-id-173605

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  28. 3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

    Plasmid
    #68405
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
    FlpO
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Promoter
    Synthetic Probasin ARRx2 and U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68405-plasmid-map-sequence-id-119955

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  29. pU6-SacB-Cas9-T2A-mCherry_(BbsI)

    Plasmid
    #117070
    Purpose
    Encodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selection
    Depositor
    Kim Failor
    Inserts
    Cas9
    SacB
    Use
    CRISPR
    Tags
    3x FLAG, NLS, and T2A-mCherry
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    117070-plasmid-map-sequence-id-231696

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  30. pUAS-kkv-R896K::NG

    Plasmid
    #138957
    Purpose
    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv-R896K::NG (kkv Fly)
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    R896K
    Promoter
    UAS
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138957-plasmid-map-sequence-id-274787

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  32. CXXC1

    Plasmid
    #32045
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CXXC1 (CXXC1 Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    CXXC domain (amino acids residues 161-222)
    Available Since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available Since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  34. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid
    #209837
    Purpose
    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1
    Depositor
    Tim Brown
    Inserts
    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    Tags
    x3 MycTag and x6 HisTag
    Expression
    Mammalian
    Promoter
    hEF1-HTLV promoter
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    209837-plasmid-map-sequence-id-414818

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  35. AAV-SYN-eGFP-RAB11

    Plasmid
    #203731
    Purpose
    AAV vector plasmid expressing human RAB11 fused to eGFP under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    RAB11 (RAB11A Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    Human synapsin promoter
    Available Since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203731-plasmid-map-sequence-id-402636

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  36. ATM_N_FS_v2_Donor

    Plasmid
    #214815
    Purpose
    N-terminal tagging of human ATM with 3xFLAG_Twin_Strep
    Depositor
    Yannick Doyon
    Insert
    3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG_2xStrep
    Expression
    Mammalian
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    214815-plasmid-map-sequence-id-422878

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  37. pFP.R400

    Plasmid
    #138175
    Purpose
    Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(GGK).NpuSsp*.(CWN)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138175-plasmid-map-sequence-id-296599

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  38. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3B (DNMT3B Human)
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Available Since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Available Since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  45. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

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  46. HDGF2

    Plasmid
    #32021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDGF2 (HDGFL2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acid residues 1-93)
    Promoter
    T7
    Available Since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  47. CD44I-c001

    Plasmid
    #175448
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175448-plasmid-map-sequence-id-353140

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  48. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Baisong Lu
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Expression
    Mammalian
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    132556-plasmid-map-sequence-id-261537

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Showing: 7551 - 7600 of 9302 results