We narrowed to 17,638 results for: URE

Showing: 7551 - 7600 of 17638 results

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actc1b-mKate2-GPI-pA

Plasmid

#109490

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-GPI-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFOX13-Tier1-PhCMV-tGFP

Plasmid

#169510

Purpose

Tier-1 vector encoding PhCMV-driven green fluorescent protein turbo GFP (PhCMV-tGFP-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven turbo green fluorescent protein

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCellFree_G06_ZIKV NS2A

Plasmid

#145353

Purpose

Cell-free expression vector for Zika virus NS2A protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

mCherry and His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Tags

His and mCherry

Expression

Bacterial

Promoter

T7- CMV/CAG

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5b

Plasmid

#109650

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11bb

Plasmid

#109597

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11bb (rab11bb Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11a

Plasmid

#109594

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11a (rab11a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11al

Plasmid

#109595

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

E275G, I287V and R294G

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKV PrM

Plasmid

#145350

Purpose

Cell-free expression vector for Zika virus prM protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV PrM

Tags

mCherry and His

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-MsCHI

Plasmid

#73402

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Tags

NLS-GFP

Expression

Yeast

Promoter

Pnmt1

Available Since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-MsCHI

Plasmid

#73392

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
MsCHI

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d_GgPCFT

Plasmid

#165414

Purpose

expression GgPCFT protein with C terminally tagged GFP-His tag cleavable with TEV protease.

Depositor

Simon Newstead

Article

Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

Insert

GgPCFT (SLC46A1 Chicken, Synthetic)

Tags

GFP-His8

Expression

Yeast

Promoter

GAL1

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAa

Plasmid

#109577

Purpose

Multisite gateway vector for 3' tagging with RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin F463A

Plasmid

#45975

Depositor

Kalle Gehring

Article

Trempe et al Science. 2013 May 9.

Insert

Parkin F463A (Prkn Rat)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

tac

Available Since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBB-80

Plasmid

#8814

Depositor

Cynthia Wolberger

Article

Batchelor et al Science 1998 Feb 13;279(5353):1037-41.

Insert

GABPa1 (318-430) (Gabpa Mouse)

Expression

Bacterial

Available Since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z18

Plasmid

#120061

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mKeima8.5

Use

Pcr template

Tags

mKeima8.5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET21-MRPS2

Plasmid

#31342

Depositor

Linda Spremulli

Article

Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

Insert

mitochondrial ribosomal protein S2 mature form (MRPS2 Human)

Tags

His

Expression

Bacterial

Mutation

Encodes the predicted mature form for incorporati…

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
TLS 3: TLS.pRSET.C

Plasmid

#21829

Depositor

David Ron

Article

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pIS47 C20orf139 3'UTR

Plasmid

#14489

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

C20orf139 3'UTR (SRXN1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pattB-3xFlag-Loqs-PD

Plasmid

#41110

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Loquacious-PD (loqs Fly)

Tags

3xFlag

Expression

Insect

Available Since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pRU205

Plasmid

#167685

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCpf1

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU321

Plasmid

#167681

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU320

Plasmid

#167682

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::asCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES2-eGFP-Ofd1-Myc-S75F

Plasmid

#24580

Depositor

Jeremy Reiter

Article

Singla et al Dev Cell. 2010 Mar 16. 18(3):410-24.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Mutation

serine 75 changed to phenylalanine (codon TCT cha…

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS314-MDM2

Plasmid

#18654

Depositor

Bert Vogelstein

Article

Oliner et al Nature. 1993 Apr 29. 362(6423):857-60.

Insert

MDM2 (MDM2 Human)

Tags

Gal1 promoter

Expression

Yeast

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pJOG331

Plasmid

#105408

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

6xHA tag fused to Twin-Strep tag

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
4E2H.210_LUMABS

Plasmid

#155209

Purpose

Expresses 4E2H.210_LUMABS sensor in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210_LUMABS

Tags

6x His Tag

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab3aa

Plasmid

#109703

Purpose

Multisite gateway vector for 3' tagging with rab3aa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab3aa (rab3aa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1935

Plasmid

#127203

Purpose

Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1

Use

Synthetic Biology

Available Since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFS217-USP44_MSCV-TAP

Plasmid

#43896

Purpose

Retroviral expression of USP44 in mammalian cells

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Retroviral

Tags

FLAG, HA, and HIS

Expression

Mammalian

Available Since

May 6, 2013

Availability

Academic Institutions and Nonprofits only
Tip alpha (21-192)

Plasmid

#23157

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

deleted amino acids 1-20

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pAN1232

Plasmid

#127164

Purpose

Vector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)

Depositor

Hana El-Samad

Article

Ng et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1

Use

Synthetic Biology

Available Since

Feb. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry

Plasmid

#68279

Purpose

SKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Far1DS NLS mCherry

Tags

mCherry

Expression

Yeast

Promoter

RPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab5b

Plasmid

#109718

Purpose

Multisite gateway vector for 3' tagging with rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab7b

Plasmid

#109724

Purpose

Multisite gateway vector for 3' tagging with rab7b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab7b (rab7b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab11al

Plasmid

#109663

Purpose

Multisite gateway vector for 3' tagging with rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

Truncated isoform

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine

Plasmid

#68278

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrine

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCitrine

Tags

mCitrine

Expression

Yeast

Promoter

pRPS2

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab6a

Plasmid

#109720

Purpose

Multisite gateway vector for 3' tagging with rab6a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6a (rab6a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK032

Plasmid

#105412

Purpose

Synthetic biology

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

Hormone-binding domain (glucocorticoid receptor)

Use

Synthetic Biology

Mutation

BsaI/ BpiI restriction sites removed

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
psecBFP2

Plasmid

#62230

Purpose

encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

Depositor

Erik Snapp

Article

Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

Insert

secBFP2

Tags

secbfp2

Expression

Mammalian

Promoter

cmv

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
p3E-rab6ba

Plasmid

#109721

Purpose

Multisite gateway vector for 3' tagging with rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab6bb

Plasmid

#109722

Purpose

Multisite gateway vector for 3' tagging with rab6bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6bb (rab6bb Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-actn2

Plasmid

#109550

Purpose

Multisite gateway vector for 5' tagging with alpha actinin 2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

alpha actinin 2

Use

Zebrafish plasmids

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN1572

Plasmid

#127202

Purpose

Dual inducible expression of Venus-degronSwitchA and KeyA-BFP

Depositor

Hana El-Samad

Article

Langan et al Nature. 2019 Jul 24. pii: 10.1038/s41586

Insert

pGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1

Use

Synthetic Biology

Available Since

Aug. 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Pacsin2

Plasmid

#109587

Purpose

Multisite gateway vector for 3' tagging with Pacsin2

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Pacsin2 (pacsin2 Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-Dnm2b

Plasmid

#109573

Purpose

Multisite gateway vector for 3' tagging with Dynamin2b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dynamin2b (dnm2b Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7551 - 7600 of 17638 results

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We narrowed to 17,638 results for: URE