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pBKWH-p53

Plasmid

#133900

Purpose

Positive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

p53 (Trp53 Mouse)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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piggybac-EF1-H2AZ-halo

Plasmid

#197907

Purpose

single-particle tracking for H2AZ

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

H2az2 (H2az2 Mouse)

Tags

Halotag

Expression

Mammalian

Promoter

EF1

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pGEM mouse islet1 [TJ#28]

Plasmid

#16270

Depositor

Thomas Jessell

Insert

islet1 (Isl1 Mouse)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
attB-GFP-Dnmt3b1-Poly(A)

Plasmid

#65531

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 1

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe RFP1-Smad2 neo

Plasmid

#58491

Purpose

mammalian expression of mRFP1-tagged mouse Smad2

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

Smad2 (Smad2 Mouse)

Tags

mRFP1

Expression

Mammalian

Available Since

Aug. 19, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-miRFP670-2

Plasmid

#197201

Purpose

Expresses the protein of miRFP670-2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP670-2

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E IRES GFP

Plasmid

#18761

Depositor

Scott Lowe

Article

Wendel et al Nature. 2004 Mar 18. 428(6980):332-7.

Insert

eIF4E (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
NPY/Neuropeptide Y scFv [L115/13]

Plasmid

#190505

Purpose

Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Enh eGFP Reporter-muKC2-X11

Plasmid

#59235

Purpose

Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.

Depositor

Benjamin Yu

Insert

Krt7-ex2

Use

Lentiviral

Promoter

Mu Hsp68 minimal promoter

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
mPlum-MyosinIIA-C-18

Plasmid

#55989

Purpose

Localization: Cytoskeleton, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

MyosinIIA (Myh9 Mouse)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pmSfrp2

Plasmid

#31249

Depositor

Jeremy Nathans

Article

Rattner et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

Insert

Sfrp2 (Sfrp2 Mouse)

Use

Cdna for in situ hybridization

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
Anti-IRSp53/BAIAP2 [L117/1R]

Plasmid

#177462

Purpose

Mammalian Expression Plasmid of anti-IRSp53/BAIAP2 (Human). Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-IRSp53/BAIAP2 (Homo sapiens) recombinant mouse monoclonal antibody (BAIAP2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHLuc Control

Plasmid

#141091

Purpose

pH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEP

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-eGFP-Nanoluc

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Alpha4 [N398A/34R]

Plasmid

#177543

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Alpha4 (Rat). Derived from hybridoma N398A/34.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Alpha4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Gabra4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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MIG(F)-CRel

Plasmid

#26984

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

CRel (Rel Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only
pL452-Sf3b1-K700E

Plasmid

#90425

Purpose

selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)

Depositor

Alex Minella

Article

Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519

Insert

Sf3b1 - left and right homology arms (Sf3b1 Mouse)

Use

Homology-directed repair vector

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Brn2

Plasmid

#32925

Purpose

Retroviral expression of mouse Brn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Brn2 (Pou3f2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

Plasmid

#189792

Purpose

Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Mouse, Human)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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