We narrowed to 5,180 results for: pcDNA3.1+

1. pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

   Plasmid

   #166805

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   LL193EE

   Promoter

   CMV

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

   Plasmid

   #166808

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   K1277E

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

   Plasmid

   #166807

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   H1276G

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R

   Plasmid

   #166806

   Purpose

   Mammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   NLRP1 (NLRP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   P1214R

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 AURKA deltaNter

   Plasmid

   #157749

   Purpose

   Expression of AURKA without Nter in mammalian cells

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   the first 30AA of Aurora A are lacking

   Promoter

   CMV

   Available since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGAL4-DBD-OLIG2-Adel-IDR

   Plasmid

   #145249

   Purpose

   Expresses fusion of GAL4 DNA-binding domain and OLIG2-Adel IDR

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   OLIG2-Adel-IDR (OLIG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A-repeats deleted

   Promoter

   Available since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. S2 1058 AIDx no UGI

   Plasmid

   #135349

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-1058
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. S2 1058 APOBEC3 no UGI

   Plasmid

   #135350

   Purpose

   Expression of intradomain insertion of rat cytosine deaminase, rAPOBEC3, at position 1058 of nSpyCas9 lacking uracil DNA glycosylase inhibitor.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-rAPOBEC3-ID-1058
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. S1 1029 AIDx with UGI

    Plasmid

    #135354

    Purpose

    Expression of nSpyCas9 encoding truncated human cytosine deaminase hAIDx at amino acid 1029 and uracil DNA glycosylase inhibitor (UGI) at the C terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    hAIDx-nSpyCas9 aa-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. S1 1029 CP ABEMax strategy 2

    Plasmid

    #135358

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding de-coupled ABEMax (wildtype Tada monomer at N terminus, evolved Tada monomer at C terminus)

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    WT Tada-nSpyCas9 aa 1029-evolved Tada
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. S1 1029 CP ABEMax strategy 3

    Plasmid

    #135359

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the C-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    1029 CP nSpyCas9 ABEMax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. S1 1029 miniABEMax V82G strategy1

    Plasmid

    #135360

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    mini ABEMax 1029 nSpyCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. S1 1029 V82G ABEMax Strategy 3

    Plasmid

    #135363

    Purpose

    Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding miniABEMax (evolved Tada monomer with V82G) at the N-terminus

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    1029 V82G ABEMax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGAL4-DBD-HOXD11wt-IDR

    Plasmid

    #145242

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD11wt-IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD11wt-IDR (HOXD11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGAL4-DBD-HOXD11-Adel-IDR

    Plasmid

    #145241

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD11-Adel-IDR (HOXD11 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGAL4-DBD-HOXA13+7A-IDR

    Plasmid

    #145239

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13+7A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGAL4-DBD-HOXA13-44A-IDR

    Plasmid

    #145237

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-44A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-44A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGAL4-DBD-HOXA13-15A-IDR

    Plasmid

    #145236

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXA13-15A IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13-15A-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGAL4-DBD-HEY2wt-IDR

    Plasmid

    #145235

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HEY2wt-IDR (HEY2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGAL4-DBD-GSX2wt-IDR

    Plasmid

    #145233

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2wt-IDR (GSX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGAL4-DBD-EOMES-Adel-IDR

    Plasmid

    #145230

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    EOMES-Adel-IDR (EOMES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGAL4-DBD-HOXD13wt-IDR

    Plasmid

    #145244

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXD13wt-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGAL4-DBD-TBX1-Adel-IDR

    Plasmid

    #145253

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and TBX1-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    TBX1-Adel-IDR (TBX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGAL4-DBD-GSX2-Adel-IDR

    Plasmid

    #145232

    Purpose

    Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    GSX2-Adel-IDR (GSX2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A-repeats deleted

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPK-352

    Plasmid

    #157922

    Purpose

    pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    tPcyA
    IRES
    HO1
    FD
    FNR
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    Mitochondrial Targeting Sequence, Mitochondrial T…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-Mm_Plk5(300-595) PBD domain

    Plasmid

    #136316

    Purpose

    Mammalian expression of mouse Plk5-D300 (PBD domain 300-595) with N-terminal EGFP

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    fragment 300-595

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-Mm_Plk5(D168A)

    Plasmid

    #136318

    Purpose

    Mammalian expression of mouse Plk5-KD (D168A) with N-terminal EGFP

    Depositor

    Marcos Malumbres, Guillermo de Cárcer

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (Plk5 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D168A

    Promoter

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-sPDGFRalpha-Fc (Legacy)

    Plasmid

    #136437

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Use

    Tags

    Fc region of IgG1 (a.a. Pro-217 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524)

    Promoter

    CMV

    Available since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-sACE2v5

    Plasmid

    #145152

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge