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pHR-mCherry-p2A-LIC1 G domain

Plasmid

#74602

Purpose

expresses LIC1 in mammalian cells; aa 1-389, untagged but cells indicate expression with diffuse mCherry

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Promoter

SFFV

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2 RK

Plasmid

#46455

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist RK (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR-actin-G168D,Y169D

Plasmid

#118384

Purpose

Gateway compatible donor vector with actin-G168D,Y169D.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

G168D,Y169D

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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NMRK2 gRNA (BRDN0001145387)

Plasmid

#77130

Purpose

3rd generation lentiviral gRNA plasmid targeting human NMRK2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NMRK2 (NMRK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Mist-SH2

Plasmid

#46454

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Mist (Clnk Mouse)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 300-435

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Hook3(1-522)-GFP-Psc-StrepII

Plasmid

#111861

Purpose

C-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

Hook3(1-552)-GFP (HOOK3 Human)

Tags

GFP, Psc tag, and Strep tag

Expression

Insect

Mutation

Optimised for expression in SF9 cells

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human LIC1 G domain

Plasmid

#74598

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 1-389

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

human dynein light intermediate chain 1 amino acids 1-389 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-IB-H2B-PA-mCherry

Plasmid

#247339

Purpose

Expresses photoactivatable H2B-Cherry under CAG promoter and can be randomly integrated by PiggyBac transposase

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform-2)

Plasmid

#245901

Purpose

Mammalian expression of CDKL5 isoform2 (1-1030) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-1 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-CDKL5(kinase)

Plasmid

#245902

Purpose

Mammalian expression of CDKL5 kinase domain (1-311) with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D

Promoter

CMV

Available Since

Nov. 7, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1α-H2B-PAmCherry-FRT

Plasmid

#247337

Purpose

Expresses photoactivatable H2B-Cherry under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

PAmCherry

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-CDKL5(isoform1)_L960D

Plasmid

#245900

Purpose

Mammalian expression of CDKL5 isoform1 (1-960) L960D with N-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP-Avitag

Expression

Mammalian

Mutation

N8D, E569G, L960D

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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CDKL5(isoform1)-GFP

Plasmid

#245903

Purpose

Mammalian expression of CDKL5 isoform1 (1-960) with C-terminal GFP

Depositor

Ben Goult

Article

Otani et al bioRxiv 2025.08.27.672337; doi: https://

Insert

CDKL5-2 (CDKL5 Human)

Tags

GFP

Expression

Mammalian

Mutation

N8D, E569G

Promoter

CMV

Available Since

Oct. 23, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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M79

Plasmid

#242631

Purpose

MYC knockdown

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYCdeltaCTD (MYC Human)

Use

RNAi

Tags

V5

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M91

Plasmid

#242635

Purpose

lower MYCdeltaCTD expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYCdeltaCTD (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M88

Plasmid

#242634

Purpose

MYCdeltaCTD expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

EGFP-MYCdeltaCTD (MYC Human)

Expression

Mammalian

Mutation

delta354-439

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M93

Plasmid

#242637

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYC (MYC Human)

Use

RNAi

Tags

V5

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M99

Plasmid

#242638

Purpose

MYC knockdown + rescue

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIIIa (MYC Human)

Use

RNAi

Tags

V5

Mutation

I189A,V193A,V194A,F195S,L199A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M92

Plasmid

#242636

Purpose

lower MYC expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

KKKK-V5-MYC (MYC Human)

Tags

V5

Expression

Mammalian

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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M76B

Plasmid

#242628

Purpose

MYCMutMBIV expression

Depositor

Alain Nepveu

Article

Faraco et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

V5-MYCMutMBIV (MYC Human)

Tags

V5

Expression

Mammalian

Mutation

A310E,A311D,P312G,P313G,R315A,K316A,D317S

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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