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TOMM20-CoilE-mCherry

Plasmid

#181995

Purpose

CoilE genetically encoded tag on TOMM2--mCherry, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

TOMM20-CoilE-mCherry

Tags

mCherry

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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AraC-pHG165c WT

Plasmid

#170114

Purpose

Constitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.

Depositor

Liskin Swint-Kruse

Article

Picard et al Proteins. 2021 Aug 8. doi: 10.1002/prot.

Insert

AraC

Expression

Bacterial

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pRS405 VAC8-G2A-GFP

Plasmid

#166837

Purpose

Expresses a myristoylation mutant Vac8-GFP in yeasts, mutation is Gly2Ala

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554

Insert

VAC8 (VAC8 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

G2A

Promoter

VAC8 promoter

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-1

Plasmid

#136385

Purpose

Expresses DANCER-1 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-1

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L5A-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-11a (GB1 WT - A34F/V39L)

Plasmid

#136610

Purpose

Expresses GB1 WT double mutant (A34F/V39L)

Depositor

Roberto Chica

Article

Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

Insert

GB1 WT double mutant (A34F/V39L)

Tags

6x-Histag

Expression

Bacterial

Mutation

Mutation from GB1 WT: A34F - V39L

Promoter

T7 promoter

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3b_007

Plasmid

#123788

Purpose

Encodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

bPAC

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0747

Plasmid

#133481

Purpose

Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0748

Plasmid

#133482

Purpose

Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0605

Plasmid

#133503

Purpose

Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0746

Plasmid

#133480

Purpose

Expression in fission yeast from promoter prga3 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB6637

Plasmid

#106164

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6681 into Yarrowia lipolytica chromosomal location IntE_3, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC104650.1-1/1

Plasmid

#91541

Purpose

Protein expression and purification of human SH3 domain construct AC104650.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC104650.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AC093799.1-1/1

Plasmid

#91495

Purpose

Protein expression and purification of human SH3 domain construct AC093799.1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AC093799.1-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-2/2

Plasmid

#91382

Purpose

Protein expression and purification of human SH3 domain construct OTOR-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-2/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_OTOR-1/2

Plasmid

#91328

Purpose

Protein expression and purification of human SH3 domain construct OTOR-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

OTOR-1/2

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_RIMBP3B-1/1

Plasmid

#91299

Purpose

Protein expression and purification of human SH3 domain construct RIMBP3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

RIMBP3B-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_LOC440461-1/1

Plasmid

#91309

Purpose

Protein expression and purification of human SH3 domain construct LOC440461-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

LOC440461-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_C1orf168-1/1

Plasmid

#91295

Purpose

Protein expression and purification of human SH3 domain construct C1orf168-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

C1orf168-1/1

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTd-4-15-13-1

Plasmid

#63826

Purpose

This plasmid is designed to validate the biophysical model of translational coupling

Depositor

Howard Salis

Article

Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

Insert

CFP, mRFP1 and GFPmut3b

Expression

Bacterial

Mutation

The intergenic distance between each gene is -4 n…

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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