161,704 results

1. pCold TDP43 WT

   Plasmid

   #27451

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Factor Xa, GST, and His6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET FLIPPi-30m

   Plasmid

   #13556

   Depositor

   Wolf Frommer

   Article

   Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

   Insert

   FLIPPi T22A
   

   Use

   Tags

   ECFP and EYFP

   Expression

   Bacterial

   Mutation

   T22A in binding protein

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEM415-ldhL-mRFP1

   Plasmid

   #99842

   Purpose

   Expression of RFP in Lactobacillus

   Depositor

   Sujin Bao

   Article

   Bao et al PLoS One. 2013;8(3):e60007. doi: 10.1371

   Insert

   monomeric red fluorescent protein
   

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   ldhl

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. TCARZ-CMV-GFP-PAmin-GF3-R4A22

   Plasmid

   #203768

   Purpose

   For production of template RNA for use in PRINT, GFP under CMV promoter

   Depositor

   Kathleen Collins

   Article

   Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

   Insert

   PRINT template RNA for GFP
   

   Use

   Ivt template

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK265 (RAD21-mAC Hygro)

   Plasmid

   #140539

   Purpose

   RAD21 tagging with mAID-Clover

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   RAD21 (RAD21 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_EF1a_Flag_EGFP

   Plasmid

   #183117

   Purpose

   Expresses flag-tagged EGFP in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLKO.1 3xHA-KillerRed-OMP25

   Plasmid

   #174544

   Purpose

   localizes KillerRed to mitochondria

   Depositor

   Minna Roh-Johnson

   Article

   Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

   Insert

   3xHA-KillerRed-OMP25
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-hISG15

   Plasmid

   #12447

   Depositor

   Dong-Er Zhang

   Article

   Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

   Insert

   ISG15 fragment (ISG15 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ISG15 up to LRLRGG

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. FUGW-eGFP-ZEB1 3'UTR

   Plasmid

   #81018

   Purpose

   FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   Zeb1 3'UTR (UTS2R Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHRIG-Akt3

    Plasmid

    #53596

    Purpose

    lentiviral expression of constitutively active human Akt3 and EGFP

    Depositor

    Heng Zhao

    Article

    Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

    Insert

    cAkt3 (AKT3 Human)
    

    Use

    Lentiviral

    Tags

    HA and IRES-EGFP

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

11. GW1-PercevalHR

    Plasmid

    #49082

    Purpose

    Mammalian expression vector (CMV promoter) of PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

    Depositor

    Gary Yellen

    Article

    Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

    Insert

    PercevalHR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti sfGFP-LAMP1-mCherry (pHLARE)

    Plasmid

    #164478

    Purpose

    Lysosomal pH biosensor consisting of sfGFP-rat Lamp1-mCherry. Plasmid for lentivirus production.

    Depositor

    Diane Barber

    Article

    Webb et al Mol Biol Cell. 2020 Nov 25:mbcE20060383.

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry and superfolder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MIG-Luciferase-IRES-GFP

    Plasmid

    #75021

    Purpose

    Encodes the expression of Luciferase and GFP

    Depositor

    Saradhi Mallampati

    Insert

    Luciferase
    

    Use

    Luciferase and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Puro_hPGK1_EBFP_Donor

    Plasmid

    #178089

    Purpose

    HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EBFP (T65S/F145Y)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Use

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. eSrtA(2A-9) pET29b

    Plasmid

    #75145

    Purpose

    reprogrammed sortase A variant that recognizes alternative substrate LAXTG with high activity and specificity

    Depositor

    David Liu

    Article

    Dorr et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

    Insert

    reprogrammed sortase A variant
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    S102C A104H E105D K138P K152I N160K K162H T164N K…

    Promoter

    T7

    Available since

    July 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET FLIPPi-200u

    Plasmid

    #13555

    Depositor

    Wolf Frommer

    Article

    Gu et al FEBS Lett. 2006 Oct 30. 580(25):5885-93.

    Insert

    FLIPPi G162A
    

    Use

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    G162A in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

    Plasmid

    #225753

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid

    #217971

    Purpose

    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.

    Depositor

    Robert Piper

    Insert

    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMEH371 CD63 HA

    Plasmid

    #168220

    Purpose

    Mammalian expression of tetraspanin CD63 fused to a C-terminal HA tag

    Depositor

    Joshua Leonard

    Article

    Hung et al J Extracell Vesicles. 2016 May 13;5:3102

    Insert

    CD63 (CD63 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

    Plasmid

    #140086

    Purpose

    Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    LbCpf1 (LbCas12a) crRNA backbone
    

    Use

    Lentiviral

    Tags

    EF1a-Puro-T2A-BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSBK.079

    Plasmid

    #187218

    Purpose

    Expresses Eco1 RT and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 Reverse Transcriptase
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23115 and T7/lac

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3

    Plasmid

    #170163

    Purpose

    Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChroME2s-P2A-H2B-mRuby3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-GFP/Cre

    Plasmid

    #49056

    Purpose

    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GFP-BAZ1A

    Plasmid

    #65371

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    BAZ1A (BAZ1A Human)
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    796I compared to all reference sequences

    Promoter

    CMV

    Available since

    June 15, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3.1-HA AKT1

    Plasmid

    #78778

    Purpose

    To overexpress AKT1 in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2015 Jul 17;6:7769. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-hLC3B-pcDNA3.1

    Plasmid

    #40827

    Depositor

    David Rubinsztein

    Article

    Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

    Insert

    LC3B (MAP1LC3B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. HDAC3 Flag

    Plasmid

    #13819

    Purpose

    Mammalian expression of human histone deacetylase 3 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC3 (HDAC3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge