163,606 results

1. pTOM20-ddGFP-B

   Plasmid

   #40291

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   TOMM20 (TOMM20 Human)
   

   Use

   Tags

   ddGFP-B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-PDi-CRISPRn

   Plasmid

   #73500

   Purpose

   Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

   Depositor

   Bruce Conklin

   Article

   Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

   Inserts

   Cas9
   rtTA
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAT001

   Plasmid

   #171061

   Purpose

   Plasmid for expressing HIV-1 envelope glycoprotein

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Insert

   HIV-1 Env
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. PyronicSF-mRuby2/pBI-CMV1

   Plasmid

   #124830

   Purpose

   Highly resposive GFP-based pyruvate nanosensor.

   Depositor

   Luis Felipe Barros

   Article

   Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

   Inserts

   PyronicSF
   mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Watermelon backbone

   Plasmid

   #155258

   Purpose

   Can be used to generate a Watermelon library

   Depositor

   Joan Brugge, Aviv Regev

   Article

   Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. VSV.G

   Plasmid

   #14888

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   VSV-G Envelope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. Rich-pT7

   Plasmid

   #108445

   Purpose

   This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

   Depositor

   Richard Bélanger

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   pT7

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXL010-Wnt dual (GFP-Fire) reporter

   Plasmid

   #40588

   Depositor

   Sean Palecek

   Article

   Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

   Insert

   Wnt reporter sequences
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Wnt promoter

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSJX022

   Plasmid

   #232325

   Purpose

   Genome editing in Caulobacter crescentus

   Depositor

   Wei Zhao

   Article

   Sun et al bioRxiv 2024.12.02.626314

   Inserts

   sgRNA
   SpCas9M
   

   Use

   CRISPR

   Tags

   H-arms and cat

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23119 and Pvan

   Available Since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSJX016

    Plasmid

    #232324

    Purpose

    Genome editing in Caulobacter crescentus

    Depositor

    Wei Zhao

    Article

    Sun et al bioRxiv 2024.12.02.626314

    Inserts

    sgRNA
    SpCas9M
    

    Use

    CRISPR

    Tags

    H-arms and sfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119 and Pvan

    Available Since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE6f

    Plasmid

    #207856

    Purpose

    Mammalian expression of PE6f prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6f
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, E471K, H99R, I632V, H721Y, D645N, K91…

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-FLEX-TeLC-P2A-EYFP-WPRE

    Plasmid

    #135391

    Purpose

    Cre-dependent Tetanus toxin light chain construct for conditional silencing of neurons.

    Depositor

    Fan Wang

    Insert

    TeLC-P2A-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP-Kif2A

    Plasmid

    #52401

    Purpose

    Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

    Depositor

    Gohta Goshima, Ryota Uehara

    Article

    Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

    Insert

    Kif2A (KIF2A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSecUAG-Evol2

    Plasmid

    #163148

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons

    Depositor

    Dieter Söll

    Article

    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

    Inserts

    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG), Cys…

    Promoter

    EM7, araBAD, native As SelD promoter, and native …

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

    Plasmid

    #208377

    Purpose

    Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

    Depositor

    Daniel Hawiger

    Article

    Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human CMV

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. alpha7nACHR_Nacho_mCherry

    Plasmid

    #182939

    Purpose

    alpha7 nACHR expression in mammalian cell lines

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Synthesized

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. lentiCRISPR-v2-HygR-mCherry

    Plasmid

    #167189

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and mCherry

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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22. p2xSTAT6-Luc2P

    Plasmid

    #35486

    Depositor

    Axel Nohturfft

    Insert

    STAT6 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    2x Stat6-c/EBP

    Available Since

    March 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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23. PiggyBac-TetO-En1-t2a-Foxa2-hygro

    Plasmid

    #176483

    Purpose

    TetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    En1-t2a-FoxA2 (FOXA2, En1 Human, Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available Since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. 4XCLEAR-luciferase reporter

    Plasmid

    #66800

    Purpose

    Reporter for activity of the CLEAR network

    Depositor

    Albert La Spada

    Article

    Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

    Insert

    4XCLEAR
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Four CLEAR elements (GTCACGTGAC) in tandem derive…

    Available Since

    Nov. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCMV BAF170-Flag

    Plasmid

    #19142

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 (SMARCC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

    Plasmid

    #161574

    Purpose

    Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHREAC

    Plasmid

    #134908

    Purpose

    Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

    Depositor

    George Lomonossoff

    Article

    Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    double 35S

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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29. mScarlet-I-LaminB

    Plasmid

    #98831

    Purpose

    In vivo visualization of the nuclear envelope (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    CytERM
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCG-Hc∆18-AA

    Plasmid

    #86559

    Purpose

    Plasmid encodes blinded measles virus H glycoprotein

    Depositor

    Jakob Reiser

    Article

    Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y463A, R515A

    Promoter

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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