We narrowed to 7,519 results for: Lif

1. L4440_BioBrick-SCR-sgRNA-A

   Plasmid

   #177811

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(+)/Luc2=tdT

   Plasmid

   #32904

   Purpose

   Expresses a fusion of firefly luciferase and tandem Tomato red fluorescent protein in mammalian cells.

   Depositor

   Christopher Contag

   Article

   Patel et al Cancer Res. 2010 Dec 15;70(24):10141-9.

   Inserts

   Firefly Luciferase
   tandem Tomato Red Fluorescent Protein
   

   Use

   Tags

   tdTomato red fluorescent protein

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-IE and n/a

   Available since

   Feb. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKD237-3a-2

   Plasmid

   #90957

   Purpose

   Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

   Depositor

   Jeffrey Tabor

   Article

   Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

   Inserts

   Thiosulfate response regulator
   Superfolder GFP
   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Bba_J23100, Bba_J23105, and PphsA

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ERroGFP_iE_C204Q_pCDNA3

   Plasmid

   #48629

   Purpose

   Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Glutamine mutation (roGFP C147, E147b, C204Q)

   Depositor

   David Ron

   Article

   Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

   Insert

   ERroGFP
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   insertion E147B, C204Q

   Promoter

   Available since

   Oct. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L4440_BioBrick-SCR-sgRNA-B

   Plasmid

   #177812

   Purpose

   Overexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)

   Depositor

   Michael Ristow

   Article

   Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

   Insert

   Scrambled sgRNA targeting sequences
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA5/FRT/TO_STf_LamB1

   Plasmid

   #181999

   Purpose

   CMV overexpression of SNAP-tag fast fusion to Lamin B1 for nuclear envelope localization in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   STf-LamB1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBSE901

   Plasmid

   #91709

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Basta resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSSA-RPG

   Plasmid

   #85932

   Purpose

   Constitutively express DsRed and conditionally express Puro and EGFP. Used to test transfection efficiency and enrich genetically modified cells

   Depositor

   Zhiying Zhang

   Article

   Ren et al Cell Mol Life Sci. 2015 Jul;72(14):2763-

   Insert

   pPB-CMV-DsRed-polyA-CAG-SSA. Puro-T2A-EGFP
   

   Use

   Dual-reporter surrogate

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBITE-EGFRvIII-F263-OKT3

   Plasmid

   #190680

   Purpose

   Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

   Depositor

   Scott McComb

   Article

   Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

   Insert

   Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET24a-LaM4-EGFP

    Plasmid

    #182641

    Purpose

    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to EGFP. LaM4-EGFP also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    anti-mCherry nanobody fused to a T7, HA, BAP and His6 epitope
    

    Use

    Tags

    BAP, EGFP, HA, His6, and T7

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C413-E45: attB4-hr5-ie1p>-attB1r

    Plasmid

    #162930

    Purpose

    Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV ie1 promoter with hr5 enhancer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBMN mCherry-OPTN(D474N)

    Plasmid

    #119683

    Purpose

    For mammalian expression of UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-3XFLAG-renilla-luciferase-beta-globin-control

    Plasmid

    #184393

    Purpose

    Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    Renilla luciferase
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Plagl2

    Plasmid

    #228061

    Purpose

    transiently express mouse Plagl2

    Depositor

    Kenneth Chen

    Article

    Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

    Insert

    Plagl2 (Plagl2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBITE-EGFRvIII-F265-OKT3

    Plasmid

    #190679

    Purpose

    Encodes an EGFRvIII-CD3 BITE protein (F265 clone)

    Depositor

    Scott McComb

    Article

    Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

    Insert

    Anti-EGFRvIII-scFv-F265-modularized-OKT3-HisTag
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICH47822_HA-Sr62TK

    Plasmid

    #233535

    Purpose

    Expresses HA-Sr62TK in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK
    

    Use

    Tags

    HA tag

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICH47822_Sr62NLRa

    Plasmid

    #233537

    Purpose

    Expresses Sr62NLRa in planta

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62NLRa
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBMN iRFP670-OPTN(D474N)

    Plasmid

    #119693

    Purpose

    For mammalian expression of iRFP-tagged UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    D474N

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT/TO_TOMM20_HaloTag7

    Plasmid

    #169330

    Purpose

    Expression of TOMM20-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag7 (TOMM20 Human)
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pQCXIP-cathepsinD-YMH

    Plasmid

    #182644

    Purpose

    Mammalian expression vector encoding a protein fusion composed of human cathepsin D fused to to one tyrosine sulfation (TS) motif (Y), myc (M) and His6 (H) epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

    Insert

    cathepsinD
    

    Use

    Retroviral

    Tags

    His6-tag, TS site from proCCK, and myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMSCV-puro-NKX2-8

    Plasmid

    #75080

    Purpose

    retroviral vector holding human NKX2-8 cDNA

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Insert

    NKX2-8 (NKX2-8 Human)
    

    Use

    Retroviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    wild-type, full-length, without native 3' UTR

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p306-BrdU-Inc

    Plasmid

    #71792

    Purpose

    BrdU-incorporating plasmid with selectable marker URA3

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-TCF4

    Plasmid

    #109144

    Purpose

    For the expression of GFP-TCF in mammalian cells

    Depositor

    Mariann Bienz

    Article

    Townsley et al Nat Cell Biol. 2004 Jul;6(7):626-33. doi

    Insert

    TCF7L2 (TCF7L2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCW-CCNE1-HA-puro

    Plasmid

    #212669

    Purpose

    Express tagged cyclin E1

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody

    Plasmid

    #138129

    Purpose

    Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-rabbit-IgG(Fc)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5/FRT/TO-EGFP-RIP2

    Plasmid

    #131201

    Purpose

    mammalian expression, tet R

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al Life Sci Alliance. 2019 Jul 26;2(4). pii

    Insert

    RIPK2 (RIPK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pWzl_CRKL-Y207F+W275L

    Plasmid

    #196022

    Purpose

    Expresses CRKL Y207F + W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pWzl_CRKL-W275L

    Plasmid

    #196019

    Purpose

    Expresses CRKL W275L

    Depositor

    William Hahn

    Article

    Cheung et al Cancer Discov. 2011 Dec;1(7):608-25. doi

    Insert

    CRKL
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p405-BrdU-Inc

    Plasmid

    #71791

    Purpose

    BrdU-incorporating plasmid with selectable marker LEU2

    Depositor

    Oscar Aparicio

    Article

    Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

    Inserts

    Thymidine Kinase
    ENT1 (SLC29A1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and GPD

    Available since

    Feb. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge