We narrowed to 9,363 results for: bli

1. Hs.NRAS G12D

   Plasmid

   #83176

   Purpose

   Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12D mutation

   Depositor

   Dominic Esposito

   Insert

   NRAS (NRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12D

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. XX01CAPN1A-c001

   Plasmid

   #175491

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-A714 (CAPN1); EF hand 1-5, H86-S268 (CAPNS1). CAPN1 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN1 / CAPNS1 (CAPN1 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. XX01CAPN2A-c001

   Plasmid

   #175492

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-L700 (CAPN2); EF hand 1-5, H86-S268 (CAPNS1). CAPN2 can be biotinylated. Codon optimised.

   Depositor

   Opher Gileadi

   Insert

   CAPN2 / CAPNS1 (CAPN2 Human)
   

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-ApaLI*-HA

   Plasmid

   #211461

   Purpose

   Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI mutant
   

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   CMV

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. C14orf169

   Plasmid

   #39114

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   C14orf169 (C14orf169 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. NanoLuc-CA2 Fusion Vector

   Plasmid

   #237187

   Purpose

   Express NanoLuc(R)-CA2 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   CA2 (CA2 Human)
   

   Tags

   NanoLuc (R)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pHN781

   Plasmid

   #178828

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A (AR Human)
   

   Tags

   His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBACgus_Sumostar/LRRK2 1867-2186

   Plasmid

   #40386

   Depositor

   Michael J Fox Foundation MJFF

   Inserts

   SUMO
   LRRK2 (LRRK2 Human)
   

   Use

   Baculovirus

   Tags

   6xHis and HisTEV

   Mutation

   WT aa1867-2186

   Promoter

   polH

   Available Since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pEC2483 (pSpy1C)

    Plasmid

    #220248

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for heterologous gene expression

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-DPP4-G418

    Plasmid

    #158453

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPROEX-TwStrp-FTO

    Plasmid

    #190265

    Purpose

    Expresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.

    Depositor

    Samie Jaffrey

    Insert

    FTO (FTO Human)
    

    Tags

    6xHis and Twin-Streptag

    Expression

    Bacterial

    Promoter

    Trc

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ADARB1-HiBiT Fusion Vector

    Plasmid

    #236892

    Purpose

    Express ADARB1-HiBiT in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    ADARB1 (ADARB1 Human)
    

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1-eIF4E

    Plasmid

    #232336

    Purpose

    Expresses human eIF4E (ENST00000450253.7, NM_001968.5) in mammalian cells

    Depositor

    Quanfu Ma

    Insert

    eIF4E (EIF4E Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-hLIF

    Plasmid

    #179711

    Purpose

    Expresses H6-MBP-LIF

    Depositor

    Mark Arbing

    Insert

    Leukemia inhibitory factor (LIF Human)
    

    Tags

    His6-MBP-TEV protease site

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX307-CTSB-puro

    Plasmid

    #158463

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-CTSB-blast

    Plasmid

    #158464

    Purpose

    Constitutive lentiviral expression of human CTSB.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    CTSB (CTSB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-FURIN-puro

    Plasmid

    #158460

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid

    #159916

    Purpose

    Mutagenesis of Penk

    Depositor

    Larry Zweifel

    Insert

    Penk (Penk Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNL [NlucP/MycMax-RE/Hygro] Vector

    Plasmid

    #236805

    Purpose

    Express MycMax response element in luciferase reporter assay

    Depositor

    Promega Corporation

    Has Service

    DNA

    Insert

    MycMax (MYC Human)
    

    Use

    Luciferase

    Tags

    NanoLuc (R)

    Mutation

    None

    Promoter

    minP

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAVLINK-EF1alpha-5' IQGAP3

    Plasmid

    #236704

    Purpose

    5' AAVLINK plasmid for IQGAP3 expression

    Depositor

    Zhonghua Lu

    Insert

    IQGAP3 (IQGAP3 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    EF1alpha

    Available Since

    Aug. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCMV-Spike

    Plasmid

    #173921

    Purpose

    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter

    Depositor

    Andreas Stuermer

    Insert

    SARS-2 Spike Protein (S Human, Synthetic, SARS-COV-2)
    

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Promoter

    standard CMV

    Available Since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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24. pcDNA_mIGg2a_Anti-HA [12CA5] v2

    Plasmid

    #236295

    Purpose

    Mammalian vector expressing Anti-HA [12CA5] variable region fused to mouse IgG2a heavy chain; to be used with pcDNA_mK_Anti-HA [12CA5] v2 light chain (Plasmid 236296) to make the antibody.

    Depositor

    Melina Fan, Addgene Research Program

    Insert

    Anti-HA [12CA5] heavy chain variable region fused to mouse IgG2a heavy chain
    

    Tags

    Mouse IgG2a Fc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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25. AASDHPPT

    Plasmid

    #39192

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AASDHPPT (AASDHPPT Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pBabe-Kras S17N

    Plasmid

    #58899

    Purpose

    Expresses human Kras with a S17N mutation

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S17N mutation

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. GST-thr-RAF1-RBD

    Plasmid

    #86033

    Purpose

    Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

    Depositor

    Dominic Esposito

    Insert

    RAF1 RBD (Ras-binding domain) (RAF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains residues 1-149

    Promoter

    T7

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLEX307-FURIN-G418

    Plasmid

    #158462

    Purpose

    Constitutive lentiviral expression of human FURIN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    FURIN (FURIN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHN1002

    Plasmid

    #178831

    Purpose

    Bacterial expression for human Saposin A

    Depositor

    Kazuhiro Abe

    Insert

    artificial gene encoding human Saposin A (AR Human)
    

    Tags

    His8, 3C

    Expression

    Bacterial

    Mutation

    Codon-usage was optimized for bacterial expressio…

    Promoter

    T7

    Available Since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCMV-SPORT6-hHMGCR1

    Plasmid

    #86085

    Purpose

    expresses the human HMG-CoA reductase isoform 1

    Depositor

    Anne Galy

    Insert

    human HMG-CoA Reductase isoform 1 (HMGCR Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

31. BASIC_SEVA_5a7_pKD46.10

    Plasmid

    #178937

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Available Since

    Feb. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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32. pHR-TRE-MyoD-P2A-miRFP703_PGK-Puro

    Plasmid

    #216666

    Purpose

    Expresses tTa-inducible MyoD and miRFP703, with puromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    MyoD (Myod1 Mouse)
    miRFP703
    PuroR
    

    Use

    Lentiviral

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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33. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

    Plasmid

    #159917

    Purpose

    Mutagenesis of Drd1

    Depositor

    Larry Zweifel

    Insert

    Drd1 (Drd1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. psd44-Lactadherin46

    Plasmid

    #46830

    Purpose

    expression of human lactadherin in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    MFGE8 (MFGE8 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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35. pT7-pelB

    Plasmid

    #157740

    Purpose

    empty vector; N-terminal PelB signal sequence; potential C-terminal His6; T7 promoter; like pET-20b, but additionally avoids added residues in final protein since cloning uses BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Tags

    PelB signal sequence and possible His6-tag (adds …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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36. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid

    #82327

    Purpose

    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)

    Depositor

    Elizabeth Stoll

    Insert

    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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37. MDH2

    Plasmid

    #38792

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MDH2 (MDH2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

38. AiP14533-pAAV-DLX2.0-minBG-DIO-SYFP2-WPRE-HGHpA (Alias: CN4533)

    Plasmid

    #222938

    Purpose

    Cre-dependent expression of SYFP2 under the DLX2.0 enhancer

    Depositor

    Jonathan Ting

    Insert

    SYFP2
    

    Use

    AAV

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAX-SCLM

    Plasmid

    #216757

    Purpose

    For strong and long-lasting expression of SuperClomeleon in mammalian cells from the hybrid CAG promoter

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    CAG (cytomegalovirus-chicken beta-actin-rabbit be…

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. K560GFP

    Plasmid

    #129761

    Purpose

    Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

    Depositor

    William Hancock

    Insert

    KHC (Khc Fly)
    

    Tags

    GFP and His6

    Expression

    Bacterial

    Mutation

    Truncation at 559

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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