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EK0395 mCherry-SG(s70587)SG-EGFP (pEAQ)

Plasmid

#191170

Purpose

EK0285 in plant-expressable format: Sensor 1 (for EK0208); mCherry marker, EGFP output.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587:EGFP (plant)

Expression

Plant

Mutation

WT

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pN1-TOMM20-Halo

Plasmid

#129348

Purpose

Halo Tag, Mitochondrial outer membrane localization

Depositor

Yasushi Okada

Article

Wang et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

Halo (Promega)

Expression

Mammalian

Available Since

April 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GCaMP6f

Plasmid

#166022

Purpose

Expresses GCaMP6f in CaMKII+ cells

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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Circular guide cloning vector

Plasmid

#180184

Purpose

Cloning vector for expressing circular RNA from a U6 promoter

Depositor

Prashant Mali

Article

Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pEGFP-Merlin

Plasmid

#84293

Purpose

Expression of wt Merlin

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLV GG hUbV-EGFP

Plasmid

#216161

Purpose

Contains a Golden-Gate cloning cassette to express up to four gRNA and EGFP

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Type

Empty backbone

Use

Lentiviral

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-PSMA

Plasmid

#11524

Depositor

Pamela Bjorkman

Article

Davis et al Proc Natl Acad Sci U S A. 2005 Apr 26. 1

Insert

prostate-specific membrane antigen (FOLH1 Human)

Tags

His

Expression

Insect

Mutation

There is a short N-terminal truncation of this pr…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBT29-splitD

Plasmid

#122599

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT29-splitC

Plasmid

#122598

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Article

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-STOP-shA1

Plasmid

#14422

Depositor

Klaus Rajewsky

Article

Oberdoerffer et al Mol Cell Biol. 2005 May . 25(10):3896-90

Insert

modified U6 promoter (U6lox), U6 STOP cassette, shA1 gene

Use

Cre/Lox and RNAi

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pBT137-splitC

Plasmid

#122605

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBT137-splitD

Plasmid

#122606

Purpose

clone M13 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

M13 bacteriophage genes

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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p4054 HA-E6AP isoform II

Plasmid

#8658

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform II (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Sox2-IP

Plasmid

#13459

Depositor

Shinya Yamanaka

Article

Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

Insert

Sox2 (Sox2 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pAB1619 hU6-BpiI-Cas13bt1-DR

Plasmid

#176314

Purpose

hU6-BpiI-Cas13bt1-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt1 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pMM119

Plasmid

#127213

Purpose

BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

GFP, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-Myc-Smurf1 delHECT

Plasmid

#13677

Depositor

Ying Zhang

Article

Yamashita et al Cell. 2005 Apr 8. 121(1):101-13.

Insert

Smurf1 deltaHect (SMURF1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Smurf1 without HECT domain (deleted amino acids 3…

Available Since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only